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Susmita Baniya

Susmita Baniya is a biotechnology researcher with academic training in molecular biology, gene cloning, yeast engineering, and bioinformatics. She is currently pursuing a Master’s degree in Biotechnology at Tribhuvan University, where her research focuses on the expression of a Plasmodium gene in yeast cells and the identification of potential natural inhibitors using computational docking approaches. Her work integrates molecular cloning, mutagenesis, and in silico screening to explore functional gene expression and potential therapeutic targets. Through this research, she applies both experimental molecular biology techniques and bioinformatics tools to study host–pathogen interactions and protein inhibition strategies. Susmita’s academic interests include molecular cloning, yeast-based expression systems, computational drug discovery, and structure-based analysis of biomolecules. Through Microbe Notes, she contributes educational articles that explain molecular biology concepts, genetic engineering techniques, and bioinformatics approaches for students and early career researchers.

Manual Plasmid Isolation (Alkaline Lysis): Principle, Reagents, Steps, Uses

June 22, 2026June 7, 2026 by Susmita Baniya
Manual Plasmid Isolation

Manual plasmid isolation is the hands-on laboratory procedure used to extract and purify plasmid DNA from cells such as bacterial and yeast cells, typically at a laboratory scale for research … Read more

Electrocompetent Cells: Significance, Mechanism, Preparation, Examples

June 15, 2026June 4, 2026 by Susmita Baniya
Electrocompetent Cells

Electrocompetent cells are bacterial cells that have been specifically prepared to take up foreign DNA, such as plasmids, via electroporation. Significance of Electrocompetent Cells Electrocompetent cells are significant to modern … Read more

Competent Cells: Applications, Preparations, Troubleshooting

April 12, 2026 by Susmita Baniya
Competent Cells

Competent cells are cells (bacteria, yeast, etc.) that have been altered to take up exogenous foreign DNA from the environment. While some bacteria can undergo transformation naturally, common laboratory strains … Read more

DH5-Alpha (DH5α) Cells Transformation: Principle, Steps

April 12, 2026March 1, 2026 by Susmita Baniya
DH5-Alpha (DH5α) Cells Transformation

What is Bacterial Transformation? Bacterial transformation is a process by which a bacterial cell takes up exogenous foreign DNA from its environment, leading to a stable hereditary change. The phenomenon … Read more

DH5-Alpha (DH5α) Cells: Properties, Mutations, Preparation, Uses

March 4, 2026February 28, 2026 by Susmita Baniya
DH5-Alpha (DH5α) Cell

DH5α is a genetically engineered Escherichia coli K-12 strain widely used in molecular biology to amplify exogenous plasmid DNA. The strain was originally constructed by  Douglas Hanahan from the parental … Read more

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