Gayathri Krishnankutty

PCR involves three main steps: Denaturation, Annealing, and Extension. To make the method more efficient and specific, variations of the method have been developed, one of which is allele-specific PCR, … Read more

Amplified Fragment Length Polymorphism PCR (AFLP-PCR) is a DNA fingerprinting technique that selectively amplifies restriction fragments generated from digested genomic DNA to detect genetic polymorphisms and create unique genomic fingerprints. … Read more

Anchored PCR is a modified PCR technique used to amplify and characterize unknown DNA sequences adjacent to a known DNA region by using one sequence-specific primer and one non-specific anchor … Read more

Polymerase Chain Reaction is a thermal process used for the amplification of the gene of interest. Invented in 1983 by Kary Mullis, this method works on the principle that when … Read more

The Polymerase Chain Reaction is a thermal cycling process invented by Kary Mullis in 1983, used to make multiple copies of nucleic acid regions of interest. In the traditional PCR … Read more

In conventional PCR, amplification occurs at both ends of the target DNA. Some applications require specific amplification of a DNA fragment containing only one known end. These include DNA fingerprinting, … Read more

The Polymerase Chain Reaction was invented by Kary Mullis in 1983. It is a thermal process used to amplify a specific gene within a DNA sequence. The reaction works on … Read more

Polymerase Chain Reaction (PCR) is a thermal process invented by Kary Mullis (1983). It is used for amplifying the gene of interest in vivo. PCR requires a machine called a … Read more

Polymerase Chain Reaction is a thermal method invented by Kary Mullis in 1983. This method involves amplifying the gene of interest in a sequence. It uses all the required components … Read more

The accuracy of the polymerase chain reaction (PCR), a method for copying DNA, is a key factor in the proper transfer of genetic information. One of the main components responsible … Read more