Sanju Tamang

Circulating tumor DNA (ctDNA) sequencing is a method that sequences the tumor-derived ctDNA fragments circulating in the bloodstream. ctDNA levels are associated with tumor size, stage, type, treatment response, and … Read more

CLIP-seq is a method used to study RNA molecules and their interactions with associated proteins in cells. This method is used to map RNA-protein interactions which is useful for studying … Read more

Transposase-based sequencing is a method that uses enzymes called transposase for library preparation that can fragment DNA and insert specific sequencing adapters into DNA fragments at the same time. This … Read more

Fluorescent in situ sequencing (FISSEQ) is a method that is used to precisely locate and sequence multiple RNA molecules within intact cells and tissues. This method sequences RNA directly inside … Read more

Ribosome sequencing (Ribo-seq), also known as ribosome profiling, is a method that sequences ribosome-protected mRNA fragments to monitor the process of translation. Ribo-seq is also known as active mRNA translation … Read more

ATAC-Seq (Assay for Transposase-Accessible Chromatin using Sequencing) is a method used to study chromatin accessibility and gene regulation. This method identifies open chromatin regions where regulatory proteins can bind, providing … Read more

Hi-C sequencing is a next-generation sequencing (NGS) method used to study the three-dimensional (3D) structure of genomes by studying the arrangement and interactions of chromatin within the nucleus. This is … Read more

Short-read sequencing is a widely used next-generation sequencing (NGS) method that generates and sequences short DNA fragments, usually between 50 and 300 base pairs at a time. It is a … Read more

Single-cell sequencing is a method that sequences a single cell at a time to study its genome, transcriptome, epigenome, or other molecular characteristics. It is useful for studying the unique … Read more

Paired-end sequencing is a next-generation sequencing method that sequences a DNA fragment from both ends. This method produces two reads for each fragment which makes the sequencing process more accurate. … Read more