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Prashant Dahal

Prashant Dahal completed his bachelor’s degree (B.Sc.) Microbiology from Sunsari Technical College, affiliated with Tribhuvan University. He is interested in topics related to Antimicrobial resistance, the mechanism of resistance development, Infectious diseases (Pneumonia, tuberculosis, HIV, malaria, dengue), Host-pathogen interaction, Actinomycetes, fungal metabolites, and phytochemicals as novel sources of antimicrobials and Vaccines.

LAP Test- Principle, Procedure, Results, Uses

April 16, 2023 by Prashant Dahal
LAP Test

LAP test is used for the differentiation and identification of catalase-negative Gram-positive cocci bacteria. Specifically, it is used to distinguish Streptococcus, Enterococcus, Pediococcus, and Lactococcus species from Aerococcus and Leuconostoc … Read more

Lipid Hydrolysis Test (Lipase Test)- Principle, Procedure, Results

April 14, 2023 by Prashant Dahal
Lipid Hydrolysis Test

Lipid Hydrolysis Test (Lipase Test) is the biochemical test used to detect the ability of bacteria to produce exoenzyme lipase and hydrolyze lipids. Lipids are important components of the biological … Read more

Lecithinase Test (Nagler’s Reaction): Principle, Procedure, Results

June 13, 2023April 13, 2023 by Prashant Dahal
Lecithinase Test (Nagler's Reaction)

Lecithinase Test (Nagler’s Reaction) is a biochemical test for assessing bacteria’s ability to produce a toxic, phospholipase enzyme called ‘lecithinase’. Lecithinase (Phospholipase C) is a lipolytic enzyme, produced by some … Read more

Casein Hydrolysis Test- Principle, Procedure, Results

April 13, 2023 by Prashant Dahal
Result of Casein Hydrolysis Test

Casein Hydrolysis Test is the biochemical test used to determine the ability of bacteria to synthesize caseinase enzyme. Casein is the major protein (phosphoprotein) with a molecular formula of C81H125N22O39P, … Read more

Carbohydrate Fermentation Test (Sugar Fermentation Test)

May 23, 2023April 13, 2023 by Prashant Dahal
Carbohydrate fermentation test

Carbohydrate Fermentation Test is the biochemical test used to assess the ability of bacteria to ferment a specific carbohydrate and to differentiate bacteria based on their carbohydrate fermentation pattern and … Read more

KOH Test- Principle, Media, Procedure, Results, Uses

April 19, 2023April 12, 2023 by Prashant Dahal
Potassium hydroxide (KOH) test

KOH test, also known as KOH mount or KOH preparation, is a rapid test used to visualize the fungal structures in clinical samples using potassium hydroxide (KOH) as a clearing … Read more

Hydrogen Sulfide (H2S) Test: Principle, Procedure, Results

April 12, 2023 by Prashant Dahal
Hydrogen Sulfide (H2S) Test

Hydrogen Sulfide (H2S) Test is the biochemical test used to detect the ability of bacteria to produce H2S gas. Several bacteria and archaea can metabolically reduce sulfur-containing compounds to hydrogen … Read more

KIA Test- Principle, Media, Procedure, Results, Uses

April 11, 2023 by Prashant Dahal
Kligler’s Iron Agar (KIA) Test

Different bacteria have different enzyme systems and different biochemical activities to derive metabolic energy. Based on the enzyme system and biochemical characteristics, the end product of metabolism of the same … Read more

Pyruvate Broth Test- Principle, Procedure, Results, Uses

June 5, 2023April 5, 2023 by Prashant Dahal
Pyruvate Broth Test

Pyruvate Broth Test is the biochemical test used to assess the ability of bacteria to utilize pyruvate and produce metabolic acid. Two pyruvates are produced as end products during glycolysis (aerobic … Read more

Salt Tolerance Test- Principle, Procedure, Results, Uses

April 4, 2023 by Prashant Dahal
Salt Tolerance Test

Salt Tolerance Test is the biochemical test used to determine the ability of bacteria to tolerate a high salt concentration (of 6.5%). Different genera of bacteria have different requirements for … Read more

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