| Basic Characteristics | Properties (B. melitensis) |
| Capsule | Negative (-ve) |
| Catalase | Positive(+ve) |
| Flagella | Negative (-ve) |
| Gelatin Hydrolysis | Negative (-ve) |
| Gram Staining | Gram Negative (-ve) |
| H2S | Negative (-ve) |
| Hemolysis | Negative (-ve) |
| Indole | Negative (-ve) |
| Litmus Milk | Negative (-ve) |
| Motility | Negative (-ve) |
| MR (Methyl Red) | Negative (-ve) |
| Nitrate Reduction | Positive(+ve) |
| Oxidase | Positive(+ve) |
| Shape | Coccobacilli, or short rods |
| Spore | Negative (-ve) |
| Urease | Positive(+ve) |
| VP (Voges Proskauer) | Negative (-ve) |
Fermentation of |
|
| L-Arabinose | Negative (-ve) |
| Erythritol | Positive(+ve) |
| D-Galactose | Negative (-ve) |
| L- Glucose | Positive(+ve) |
| Oxidation of L- Alanine | Positive (+ve) |
| Oxidation of L-Asparagine | Positive (+ve) |
| Oxidation of L-Glutamic acid | Positive (+ve) |
| D-Ribose | Negative (-ve) |
| D-Xylose | Negative (-ve) |
Enzymatic Reactions |
|
| Arginine Dehydrolase | Negative (-ve) |
| Citrulline | Negative (-ve) |
| Glycylglycine-b-naphthylamidase | Positive (+ve) |
| Glycine-b-naphthylamidase | Positive (+ve) |
| 3-Indoxyl phosphatase | Negative (-ve) |
| Lysine | Negative (-ve) |
| Ornithine Decarboxylase | Negative (-ve) |
| p-Nitrophenyl-beta-d-galactopyranosidase | Negative (-ve) |
| p-Nitrophenyl-alpha-d-galactopyranosidase | Negative (-ve) |
| p-Nitrophenyl-N-acetyl-b-d-glucosaminidase | Positive (+ve) |
| p-Nitrophenyl-alpha-d-glucopyranosidase | Positive (+ve) |
| p-Nitrophenyl phosphatase | Positive (+ve) |
References
- BERGEY’S MANUAL OF Systematic Bacteriology. Second Edition. Volume Two. The Proteobacteria. Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria.
- Young, E. J., Gomez, C. I., Yawn, D. H., & Musher, D. M. (1979). Comparison of Brucella abortus and Brucella melitensis infections of mice and their effect on acquired cellular resistance. Infection and immunity, 26(2), 680-5.
- Barua, A., Kumar, A., Thavaselvam, D., Mangalgi, S., Prakash, A., Tiwari, S., Arora, S., Sathyaseelan, K. (2016). Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India. The Indian journal of medical research, 143(5), 652-8.
- https://www.health.ny.gov/guidance/oph/wadsworth/brucella_species.pdf
- http://www.cfsph.iastate.edu/Factsheets/pdfs/brucellosis_melitensis.pdf
- https://www.cabi.org/isc/datasheet/90734
- https://academic.oup.com/femspd/article/19/4/315/478445
- https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/brucella-melitensis
