Biochemical Test of Salmonella Typhi

Biochemical Test of Salmonella Typhi

Characteristics Salmonella Typhi
Capsule Negative (-ve)
Catalase Positive (+ve)
Citrate Negative (-ve)
Flagella Positive (+ve)
Gas Negative (-ve)
Gelatin Hydrolysis Negative (-ve)
Gram Staining Negative (-ve)
Growth in KCN Negative (-ve)
H2S Positive (+ve)
Indole Negative (-ve)
Motility Positive (+ve)
MR (Methyl Red) Positive (+ve)
MUG Test Negative (-ve)
Nitrate Reduction Positive (+ve)
Oxidase Negative (-ve)
Pigment Negative (-ve)
Shape Rod
Spore Negative (-ve)
TSIA (Triple Sugar Iron Agar) Alkali/Acid
Urease Negative (-ve)
VP (Voges Proskauer) Negative (-ve)

Fermentation of

Adonitol Negative (-ve)
Arabinose Negative (-ve)
Arabitol Negative (-ve)
Cellobiose Negative (-ve)
DNase Negative (-ve)
Dulcitol Negative (-ve)
Erythritol Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Glucose Positive (+ve)
Glycerol Negative (-ve)
Inositol Negative (-ve)
Lactose Negative (-ve)
Malonate Negative (-ve)
Maltose Positive (+ve)
Mannitol Positive (+ve)
Mannose Positive (+ve)
Melibiose Positive (+ve)
Mucate Negative (-ve)
MyoInositol Negative (-ve)
Raffinose Negative (-ve)
Rhamnose Negative (-ve)
Salicin Negative (-ve)
Sorbitol Positive (+ve)
Sucrose Negative (-ve)
Tartrate Positive (+ve)
Trehalose Positive (+ve)
Xylose Positive (+ve)

Enzymatic Reactions

Acetate Utilization Negative (-ve)
Arginine Dehydrolase Negative (-ve)
Esculin Hydrolysis Negative (-ve)
Lipase Negative (-ve)
Lysine Positive (+ve)
ONPG (β-galactosidase) Negative (-ve)
Ornithine Decarboxylase Negative (-ve)
Peroxidase Negative (-ve)
Tyrosine Hydrolysis Negative (-ve)

About Author

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Sagar Aryal, PhD

Dr. Sagar Aryal is a microbiologist, researcher, and science communicator from Nepal. He is also co-founder and Research Head of the Department of Natural Products at the Kathmandu Research Institute for Biological Sciences (KRIBS), where he leads research on natural product discovery and microbial biodiversity. Dr. Aryal earned his Ph.D. in Microbiology from Tribhuvan University, Kathmandu, Nepal, in 2025, supported by a DAAD Research Grant and a University Grants Commission (UGC) Nepal Ph.D. Fellowship. His research interests include Actinomycetes, Myxobacteria, microbial taxonomy, and natural products, particularly the discovery of bioactive compounds with potential pharmaceutical applications. He isolated and identified Nocardia nepalensis, a novel bacterial species from Nepal, contributing to understanding microbial diversity in the region. Dr. Aryal has published more than 16 research articles in international journals and contributed three book chapters to established academic publications. He has presented scientific research findings and developed international research collaborations through visits to more than 18 countries across Asia, Europe, North America, and Oceania. From 2023 to 2025, he served as the American Society for Microbiology (ASM) Young Ambassador to Nepal. Since December 2025, he has served as an Editorial Board Member of Scientific Reports, a Nature Portfolio journal. In recognition of his scientific contributions, Dr. Aryal received the NAST Science and Technology Youth Award 2025 from the Nepal Academy of Science and Technology. He previously received the Frs. Locke and Stiller Research Award (LSRA) for the 2017-2018 cycle from St. Xavier's College, Kathmandu, and a Nepal Academy of Science and Technology (NAST) Research Grant for 2017-2018. Alongside his scientific career, Dr. Aryal has more than 15 years of experience in blogging, content writing, and search engine optimization (SEO).

3 thoughts on “Biochemical Test of Salmonella Typhi”

  1. DEAR SIR HOW CAN IDENTIFY SALMONELLA TYPHI IF ON SS AGAR AND XLD AGAR PIGMENT NOT OBSERVED WHICH TEST CAN I PERFORM IN MANUALLY.
    THANKS IN ADVANCED

    Reply

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