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Saman Bhattarai

Saman Bhattarai is a microbiologist and molecular biology researcher with strong expertise in plant pathology, microbial genomics, and applied microbiology. He holds a Bachelor’s degree in Microbiology from St. Xavier’s College, Kathmandu, with a strong academic record. Saman currently works as a Research Assistant at the Molecular Biology Laboratory, National Plant Pathology Research Centre under the Nepal Agricultural Research Council. His work focuses on molecular diagnostics, whole-genome sequencing, and the genotypic and phenotypic characterization of plant pathogens and beneficial microbes. He has contributed to nationally and internationally funded projects supported by FAO, USAID, NAST, and ILRI, addressing critical agricultural challenges such as Banana Fusarium Wilt (TR4), rice blast, citrus greening, and plant viral diseases. His research experience includes qPCR assay development, MinION sequencing, metagenomics, UAV-assisted disease surveillance, and green synthesis of antimicrobial nanoparticles. Previously, he served as an Assistant Microbiologist in the agri-biotech sector, where he worked on microbial fermentation, consortium development, quality control, and GMP-aligned laboratory practices. Saman has published in international peer-reviewed journals and has several systematic reviews and first reports under review and preparation.

Plasmid Vectors: Components, Markers, Examples, Applications

March 16, 2026March 12, 2026 by Saman Bhattarai
Plasmid Vectors

In recombinant DNA technology (RDT), the terminology vector refers to a carrier that allows the transfer of genetic material from one cell to another or from one organism to another. … Read more

CRISPR-Cas Systems: Structure, Mechanism, Classification, Enzymes, Applications

March 12, 2026 by Saman Bhattarai
CRISPR-Cas systems

The CRISPR-Cas system consists of a special sequence in prokaryotes, known as clustered regularly interspaced short palindromic repeats (CRISPR) and Cas (CRISPR-associated) genes. CRISPR was first identified in Escherichia coli … Read more

Gene Cloning and Recombinant DNA Technology

March 16, 2026February 22, 2026 by Saman Bhattarai
Gene Cloning and Recombinant DNA Technology

The fundamental concepts of gene expression were based on genetic analysis of bacteria and viruses (prokaryotes only). The development of gene cloning, or molecular cloning, allowed scientists and molecular biologists … Read more

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