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Khushi Sharma

Khushi Sharma is a microbiology and biotechnology graduate with training in molecular biology, protein biochemistry, and biomedical research. She completed her Master’s degree in Biotechnology from Amity University, Lucknow, and holds a Bachelor’s degree in Microbiology from Jai Hind College, Mumbai. Her research experience includes dissertation training at the Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, where she studied protein–protein interactions between cFLIP and Calmodulin in the extrinsic pathway of apoptosis. During this work, she gained practical experience in molecular and biochemical techniques such as PCR, bacterial transformation, agarose gel electrophoresis, SDS PAGE, protein purification using Ni NTA chromatography, microbial culturing, and laboratory media preparation. Khushi has also participated in research and data curation activities at the Tata Institute of Fundamental Research, where she worked on scientific literature analysis and data organization from research publications. Her additional training includes courses in epidemiology, antimicrobial resistance in bacterial pathogens, and molecular docking approaches for drug discovery.

Severe Combined Immunodeficiency (SCID): An Overview

March 22, 2026 by Khushi Sharma
Severe Combined Immunodeficiency (SCID)

Causes of Severe Combined Immunodeficiency (SCID) Types and mechanisms of Severe Combined Immunodeficiency (SCID) X-linked SCID (SCID-X1) Adenosine Deaminase (ADA) Deficiency Recombinase-Activating Gene (RAG) Deficiency Clinical Presentation and Diagnosis of … Read more

Streptomycin: Structure, Production, Harvest, Recovery

March 22, 2026 by Khushi Sharma
Streptomycin Production

The discovery of streptomycin in 1943 by Selman Waksman and Albert Schatz was a monumental moment in medical history. As the first successful drug against tuberculosis (TB), this aminoglycoside antibiotic … Read more

Data Integrity, ALCOA++, and GxP in Pharmaceuticals

March 22, 2026March 13, 2026 by Khushi Sharma
Data integrity, ALCOA++, and GxP in the pharmaceutical industry

Data Integrity in the pharmaceutical industry is the absolute foundation for ensuring patient safety and drug quality. Principle Significance Attributable Ensures accountability. Individuals should be accountable for their actions. Legible … Read more

Ramachandran Plot: Principle, Tools, Applications, Errors

March 16, 2026March 11, 2026 by Khushi Sharma
Protein Structure

The Ramachandran plot is a fundamental tool in structural biology used to visualize the energetically allowed regions for backbone dihedral angles in a protein. Because protein folding is constrained by … Read more

R2A Media: Principle, Ingredients, Results, Applications

March 4, 2026February 27, 2026 by Khushi Sharma
R2A Media for Water Testing

Water is a pivotal solvent in many industries, including pharmaceuticals, textiles, food, and Agri-tech. Water quality influences subsequent processes; hence, it is important to conduct water analysis. There are various … Read more

Caspases and c-FLIP (Cellular FLICE-inhibitory protein)

March 22, 2026February 26, 2026 by Khushi Sharma
c-FLIP and Procaspase

What are Caspases? Caspases play a critical role in apoptosis and belong to a family of protease enzymes involved in apoptosis, inflammation, and other cellular processes. Structurally, caspases typically consist … Read more

Zero Liquid Discharge (ZLD) System: Working, Steps, Applications

March 22, 2026February 24, 2026 by Khushi Sharma
Zero Liquid Discharge System

How does Zero Liquid Discharge (ZLD) work? The working of a ZLD plant can be divided into 3 main processes: Pre-treatment Evaporation Crystallization Key Drivers for Zero Liquid Discharge (ZLD) … Read more

Trypan Blue Staining or Cell Viability Test

March 22, 2026February 20, 2026 by Khushi Sharma
Trypan Blue Staining

Trypan blue staining is a fundamental technique in cell biology that uses dye exclusion to quickly assess and differentiate viable (live) from non-viable (dead or compromised) cells within a population, … Read more

Enzyme Immobilization: Classification, Methods, Applications

March 22, 2026February 19, 2026 by Khushi Sharma
Enzyme immobilization

Enzymes are biological catalysts, primarily protein macromolecules, that act as highly efficient catalysts in living systems. Their fundamental role is to significantly accelerate (speed up) biochemical reactions without being used … Read more

The HtrA Gene and Protein: A Dual Role in Mitochondrial Quality Control and Apoptosis

March 22, 2026February 17, 2026 by Khushi Sharma
HtRA gene and protein

Apoptosis, or programmed cell death, is a crucial biological process fundamentally distinct from necrosis, which is cell death resulting from acute injury or trauma. It is a highly controlled and … Read more

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