{"id":41494,"date":"2023-08-03T15:03:16","date_gmt":"2023-08-03T09:18:16","guid":{"rendered":"https:\/\/microbenotes.com\/rapid-method-for-enumeration-of-e-coli-biotype-i\/"},"modified":"2023-08-03T15:03:16","modified_gmt":"2023-08-03T09:18:16","slug":"rapid-method-for-enumeration-of-e-coli-biotype-i","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/rapid-method-for-enumeration-of-e-coli-biotype-i\/","title":{"rendered":"Rapid Method for Enumeration of E. coli Biotype I"},"content":{"rendered":"<p><span style=\"color: #000000;\"><strong>This method is modified by Anderson and Parker.<\/strong><\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-138\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/05\/Rapid-Method-for-Enumeration-of-E.-coli-Biotype-I.jpg\" alt=\"Rapid Method for Enumeration of E. coli Biotype I\" width=\"792\" height=\"430\" \/><\/p>\n<ol>\n<li><span style=\"color: #000000;\">Prepare serial tenfold dilutions in peptone water of food.<\/span><\/li>\n<li><span style=\"color: #000000;\">Aseptically transfer sterile cellulose acetate membranes to the surface or dried glutamate agar.<\/span><\/li>\n<li><span style=\"color: #000000;\">Transfer duplicate 1.0 mL aliquots of each dilution to cellulose acetate membranes, using a sterile glass Drigalski spreader, distribute the fluid over the entire membrane (except the periphery).<\/span><\/li>\n<li><span style=\"color: #000000;\">Incubate plates 4 h at 35\u00b0C (facilitate resuscitation). <\/span><\/li>\n<li><span style=\"color: #000000;\">Transfer each membrane to tryptone bile agar plate and incubate for 18 h at 44\u00b0C.<\/span><\/li>\n<li><span style=\"color: #000000;\">Remove lids of plates. <\/span><\/li>\n<li><span style=\"color: #000000;\">Add 3 mL Kovacs reagent into each lid. <\/span><\/li>\n<li><span style=\"color: #000000;\">Remove membrane from agar and immerse in reagent for 5 min. <\/span><\/li>\n<li><span style=\"color: #000000;\">Remove membrane and drain excess reagent. <\/span><\/li>\n<li><span style=\"color: #000000;\">Dry the membranes under a UV lamp.<\/span><\/li>\n<li><span style=\"color: #000000;\">Count pink-stained colonies within 30 min.<\/span><\/li>\n<li><span style=\"color: #000000;\">Calculate the number of type I <em>E. coli<\/em> by selecting the dilution giving an average of<\/span><br \/>\n<span style=\"color: #000000;\">20\u201350 pink colonies and both membranes and multiply total by the dilution factor.<\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>This method is modified by Anderson and Parker. Prepare serial tenfold dilutions in peptone water of food. Aseptically transfer sterile cellulose acetate membranes to the surface or dried glutamate agar. &#8230; <a title=\"Rapid Method for Enumeration of E. coli Biotype I\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/rapid-method-for-enumeration-of-e-coli-biotype-i\/\" aria-label=\"Read more about Rapid Method for Enumeration of E. coli Biotype I\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":41493,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6207],"tags":[230,740,746,968,969],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/05\/Rapid-Method-for-Enumeration-of-E.-coli-Biotype-I.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":6207,"name":"Protocols","slug":"protocols","term_group":0,"term_taxonomy_id":6207,"taxonomy":"category","description":"Microbiology Protocols. Molecular Biology and Genetics Protocols. Biology and Life Sciences Protocols for undergraduate and graduate students with diagrams.","parent":0,"count":9,"filter":"raw","cat_ID":6207,"category_count":9,"category_description":"Microbiology Protocols. Molecular Biology and Genetics Protocols. Biology and Life Sciences Protocols for undergraduate and graduate students with diagrams.","cat_name":"Protocols","category_nicename":"protocols","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/41494"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=41494"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/41494\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/41493"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=41494"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=41494"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=41494"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}