{"id":39480,"date":"2022-09-21T01:54:00","date_gmt":"2022-09-20T20:09:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=39480"},"modified":"2022-09-25T02:03:08","modified_gmt":"2022-09-24T20:18:08","slug":"rt-pcr-definition-principle-enzymes-types-steps-uses","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/rt-pcr-definition-principle-enzymes-types-steps-uses\/","title":{"rendered":"RT-PCR: Definition, Principle, Enzymes, Types, Steps, Uses"},"content":{"rendered":"\n
Polymerase chain reaction (PCR)<\/a> is a temperature-dependent nucleic acid amplification technique used to amplify the DNA<\/a> or RNA<\/a> in vitro enzymatically.<\/strong><\/p>\n\n\n\n Developed by Kary Mullis and his associates at mid – 1980s, it is a very powerful and most important tool in modern biology – molecular biology and genetics. It combines the principle of nucleic acid hybridization with the principle of nucleic acid replication. Using this non-culture-based nucleic acid amplification technique, we can produce billions of copies of a single segment of DNA or RNA in a very short time.<\/p>\n\n\n\n Since its development, several modifications have been made, and now there are different types of PCR techniques available for different purposes. Reverse transcriptase PCR and Quantitative PCR (qPCR)<\/a> are the most commonly used PCR types.<\/p>\n\n\n\n Reverse transcriptase polymerase chain reaction, RT-PCR, is a type of PCR technique that enzymatically amplifies the RNA in vitro.<\/strong><\/p>\n\n\n\n It is the only type of PCR that can amplify the RNA. It uses a reverse transcriptase<\/em><\/strong> enzyme in addition to the other basic components of the PCR. <\/p>\n\n\n\n First, the sample RNA is converted to complementary DNA (cDNA) in reverse transcription, catalyzed by the reverse transcriptase enzyme. These cDNA molecules are then used as a template for amplification in the PCR process.<\/p>\n\n\n\n RT-PCR is used to analyze the mRNA or micro RNA and study gene expression. <\/p>\n\n\n\n