{"id":31848,"date":"2022-05-27T14:31:50","date_gmt":"2022-05-27T08:46:50","guid":{"rendered":"https:\/\/microbenotes.com\/?p=31848"},"modified":"2022-09-05T14:16:42","modified_gmt":"2022-09-05T08:31:42","slug":"sullivan-and-mccarthys-test","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/","title":{"rendered":"Sullivan and McCarthy\u2019s Test- Definition, Principle, Procedure, Result, Uses"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#sullivan-and-mccarthys-test-definition\" title=\"Sullivan and McCarthy\u2019s Test Definition\">Sullivan and McCarthy\u2019s Test Definition<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#objectives-of-sullivan-and-mccarthys-test\" title=\"Objectives of Sullivan and McCarthy\u2019s Test\">Objectives of Sullivan and McCarthy\u2019s Test<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#principle-of-sullivan-and-mccarthys-test\" title=\"Principle of Sullivan and McCarthy\u2019s Test\">Principle of Sullivan and McCarthy\u2019s Test<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#requirements\" title=\"Requirements\">Requirements<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#procedure-of-sullivan-and-mccarthys-test\" title=\"Procedure of Sullivan and McCarthy\u2019s Test\">Procedure of Sullivan and McCarthy\u2019s Test<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#result-and-interpretation-of-sullivan-and-mccarthys-test\" title=\"Result and Interpretation of Sullivan and McCarthy\u2019s Test\">Result and Interpretation of Sullivan and McCarthy\u2019s Test<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#uses-of-sullivan-and-mccarthys-test\" title=\"Uses of Sullivan and McCarthy\u2019s Test\">Uses of Sullivan and McCarthy\u2019s Test<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#limitations\" title=\"Limitations\">Limitations<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/#references\" title=\"References\">References<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"sullivan-and-mccarthys-test-definition\"><\/span><strong>Sullivan and McCarthy\u2019s Test <\/strong><strong>Definition<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Sullivan and McCarthy\u2019s test is a biochemical test for the detection of the <a href=\"https:\/\/microbenotes.com\/amino-acids-properties-structure-classification-and-functions\/\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>amino acid<\/strong> <\/a>methionine or methionine-containing proteins. The test is a specific test for methionine which has a high degree of specificity towards methionine as it gives negative results with all other amino acids. The test, however, gives a positive result with glycylmethionine. It is a desirable method for methionine detection as it is a rapid and simple method for the assay of methionine even if fermented extract. Sullivan and McCarthy\u2019s test is a color reaction of proteins which indicates the presence of a certain molecule by the development of a particular color with a particular reagent.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"objectives-of-sullivan-and-mccarthys-test\"><\/span><strong>Objectives of Sullivan and McCarthy\u2019s Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>To detect the presence of the amino acid methionine or methionine-containing proteins.<\/li>\n<li>To differentiate methionine from other sulfur-containing amino acids like cysteine and cystine.<\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"principle-of-sullivan-and-mccarthys-test\"><\/span><strong>Principle of Sullivan and McCarthy\u2019s Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>The Sullivan and McCarthy\u2019s test is based on the reaction between nitroprusside and alkaline solution of methionine under acidification. The test was developed when it was discovered that tryptophan and histidine both produce a red color with nitroprusside under similar conditions. Thus, acid is added to the sample solution in order to destroy any tryptophan present by the process of acid hydrolysis. Similarly, it has been stated that the addition of glycine eliminates the detection of histidine. The red color is obtained after the addition of sodium nitroprusside to an alkaline solution of methionine followed by the acidification of the reaction.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"requirements\"><\/span><strong>Requirements<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><strong>Reagent<\/strong><\/p>\n<ul>\n<li>10% sodium nitroprusside<\/li>\n<li>5N Sodium hydroxide<\/li>\n<li>6N HCl<\/li>\n<li>2% glycine<\/li>\n<li>Sample solution<\/li>\n<\/ul>\n<p><strong>Materials required<\/strong><\/p>\n<ul>\n<li>Water bath<\/li>\n<li>Ice bath<\/li>\n<li>Vortex<\/li>\n<li>Test tubes<\/li>\n<li>Test tube stand<\/li>\n<li>Pipettes<\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"procedure-of-sullivan-and-mccarthys-test\"><\/span><strong>Procedure of Sullivan and McCarthy\u2019s Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ol>\n<li>A few drops of sodium hydroxide are added to 1 ml of test solution in a test tube.<\/li>\n<li>To this, a few drops of 2% glycine and 10% sodium nitroprusside solution is added.<\/li>\n<li>The solution is then mixed together by placing it in a vortex for 2 minutes.<\/li>\n<li>The test tube is then placed in a water bath at 40\u00b0C for 15 minutes.<\/li>\n<li>The tube is then cooled in ice-cold water for 5 minutes followed by the addition of 0.5ml of 6N HCl.<\/li>\n<li>The contents are then mixed together in a vortex, and the test tube is allowed to stand at room temperature for 15 minutes. The observation is then made.<\/li>\n<\/ol>\n<h2><span class=\"ez-toc-section\" id=\"result-and-interpretation-of-sullivan-and-mccarthys-test\"><\/span><strong>Result and Interpretation of Sullivan and McCarthy\u2019s Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-31849\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Sullivan-and-McCarthys-Test.jpeg\" alt=\"Sullivan and McCarthy\u2019s Test\" width=\"900\" height=\"472\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Sullivan-and-McCarthys-Test.jpeg 900w, https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Sullivan-and-McCarthys-Test-300x157.jpeg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Sullivan-and-McCarthys-Test-768x403.jpeg 768w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/p>\n<ul>\n<li><strong>Positive result:<\/strong> The positive result is represented by the appearance of the red color. This indicates the presence of methionine.<\/li>\n<li><strong>Negative result:<\/strong> The negative result is represented by the absence of the red color. This indicates the absence of methionine.<\/li>\n<\/ul>\n<h3><span class=\"ez-toc-section\" id=\"uses-of-sullivan-and-mccarthys-test\"><\/span><strong>Uses of Sullivan and McCarthy\u2019s Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>Sullivan and McCarthy\u2019s test is used for the detection of methionine either in a free form or in proteins.<\/li>\n<li>The test can be modified for quantification where the test sample can be matched against the standard methionine solution.<\/li>\n<\/ul>\n<h3><span class=\"ez-toc-section\" id=\"limitations\"><\/span><strong>Limitations<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>The test might give a positive result with commercial leucine as it might contain some traces of methionine.<\/li>\n<li>The test might not give reproducible results with standard solutions of methionine.<\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"references\"><\/span><strong>References<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ol>\n<li>D (2012). Biochemistry. Fourteenth Edition. Academic Publishers. Kolkata.<\/li>\n<li>Tiwari, A. (2015). Practical Biochemistry. \u00a0LAP Lambert Academic Publishing.<\/li>\n<li>McCarthy T.E and Sullivan M.X (1941). A New and Highly Specific Colorimetric Test For Methionine. Journal of Biological Chemistry. 151. 635.<\/li>\n<li>Csonka F and Denton C. (1946). Methionine Determination in Proteins and Foods. J. Biol. Chem. 1946, 163:329-338.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Sullivan and McCarthy\u2019s Test Definition Sullivan and McCarthy\u2019s test is a biochemical test for the detection of the amino acid methionine or methionine-containing proteins. The test is a specific test &#8230; <a title=\"Sullivan and McCarthy\u2019s Test- Definition, Principle, Procedure, Result, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/sullivan-and-mccarthys-test\/\" aria-label=\"Read more about Sullivan and McCarthy\u2019s Test- Definition, Principle, Procedure, Result, Uses\">Read more<\/a><\/p>\n","protected":false},"author":11,"featured_media":31849,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1553],"tags":[1887,5789,5819],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Sullivan-and-McCarthys-Test.jpeg","author":{"name":"Anupama Sapkota","avatar":"https:\/\/secure.gravatar.com\/avatar\/0e9c86afc1670c49efeb260138ec905b?s=96&d=mm&r=g"},"categories":[{"term_id":1553,"name":"Biochemistry","slug":"biochemistry","term_group":0,"term_taxonomy_id":1553,"taxonomy":"category","description":"<strong>Biochemistry is a branch of science that deals with the structure, composition, and metabolism of biomolecules found in different living organisms.<\/strong>\r\n<ul>\r\n \t<li>Biochemistry is also termed \u2018biological chemistry\u2019 as it deals with the chemistry of life that utilizes techniques from analytical, inorganic, and organic chemistry.<\/li>\r\n \t<li>Metabolic processes in living beings are composed of catabolic and anabolic processes. Both of these processes involve metabolic reactions involving biochemical compounds.<\/li>\r\n \t<li>Biochemistry is also used in techniques that help to understand the interactions, metabolism, and functions of biomolecules.<\/li>\r\n \t<li>Important biomolecules like proteins, carbohydrates, fats, nucleic acid, vitamins, etc. are essential structural and physiological components of living organisms.<\/li>\r\n \t<li>The study of the biochemical composition of living beings is important as it helps to understand the molecular mechanisms of various biochemical phenomena like respiration, photosynthesis, fermentation, etc.<\/li>\r\n \t<li>The findings of these studies, in turn, can be applied in industries, nutrition, medicine, and agriculture for different purposes.<\/li>\r\n \t<li>The rate of biochemical reactions, enzymes, and the concentration of substrates and products give essential information about the organism.<\/li>\r\n \t<li>Biochemical reactions form the basis of biochemical testing that is used for the identification and differentiation of microorganisms into different groups.<\/li>\r\n \t<li>Results of studies involving different biochemical tests can be applied for the production of pharmaceutical products that selectively inhibit certain metabolic processes.<\/li>\r\n \t<li>Techniques in biochemistry have been increasingly combined with techniques from other fields like agriculture, genetic, molecular biology, and biophysics.<\/li>\r\n \t<li>Biochemistry also allows the understanding of nutritional requirements for different living organisms.<\/li>\r\n \t<li>Biochemical compounds have been increasingly used even for the diagnosis of different diseases like diabetes.<\/li>\r\n \t<li>The determination of the genetic composition of living beings is also a part of biochemistry which helps in genetic studies and diagnosis of genetic diseases.<\/li>\r\n \t<li>Biochemical analysis of different biomolecules assists in the study relating to the origin and evolution of living beings.<\/li>\r\n \t<li>An understanding of different biochemical processes helps to understand the complexity of different groups of living beings and their relationship with one another.<\/li>\r\n<\/ul>","parent":0,"count":157,"filter":"raw","cat_ID":1553,"category_count":157,"category_description":"<strong>Biochemistry is a branch of science that deals with the structure, composition, and metabolism of biomolecules found in different living organisms.<\/strong>\r\n<ul>\r\n \t<li>Biochemistry is also termed \u2018biological chemistry\u2019 as it deals with the chemistry of life that utilizes techniques from analytical, inorganic, and organic chemistry.<\/li>\r\n \t<li>Metabolic processes in living beings are composed of catabolic and anabolic processes. Both of these processes involve metabolic reactions involving biochemical compounds.<\/li>\r\n \t<li>Biochemistry is also used in techniques that help to understand the interactions, metabolism, and functions of biomolecules.<\/li>\r\n \t<li>Important biomolecules like proteins, carbohydrates, fats, nucleic acid, vitamins, etc. are essential structural and physiological components of living organisms.<\/li>\r\n \t<li>The study of the biochemical composition of living beings is important as it helps to understand the molecular mechanisms of various biochemical phenomena like respiration, photosynthesis, fermentation, etc.<\/li>\r\n \t<li>The findings of these studies, in turn, can be applied in industries, nutrition, medicine, and agriculture for different purposes.<\/li>\r\n \t<li>The rate of biochemical reactions, enzymes, and the concentration of substrates and products give essential information about the organism.<\/li>\r\n \t<li>Biochemical reactions form the basis of biochemical testing that is used for the identification and differentiation of microorganisms into different groups.<\/li>\r\n \t<li>Results of studies involving different biochemical tests can be applied for the production of pharmaceutical products that selectively inhibit certain metabolic processes.<\/li>\r\n \t<li>Techniques in biochemistry have been increasingly combined with techniques from other fields like agriculture, genetic, molecular biology, and biophysics.<\/li>\r\n \t<li>Biochemistry also allows the understanding of nutritional requirements for different living organisms.<\/li>\r\n \t<li>Biochemical compounds have been increasingly used even for the diagnosis of different diseases like diabetes.<\/li>\r\n \t<li>The determination of the genetic composition of living beings is also a part of biochemistry which helps in genetic studies and diagnosis of genetic diseases.<\/li>\r\n \t<li>Biochemical analysis of different biomolecules assists in the study relating to the origin and evolution of living beings.<\/li>\r\n \t<li>An understanding of different biochemical processes helps to understand the complexity of different groups of living beings and their relationship with one another.<\/li>\r\n<\/ul>","cat_name":"Biochemistry","category_nicename":"biochemistry","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/31848"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=31848"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/31848\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/31849"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=31848"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=31848"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=31848"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}