{"id":31553,"date":"2022-04-18T15:39:17","date_gmt":"2022-04-18T09:54:17","guid":{"rendered":"https:\/\/microbenotes.com\/?p=31553"},"modified":"2022-09-05T14:14:30","modified_gmt":"2022-09-05T08:29:30","slug":"anthrone-test","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/anthrone-test\/","title":{"rendered":"Anthrone Test- Definition, Principle, Procedure, Result, Uses"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#anthrone-test-definition\" title=\"Anthrone Test Definition\">Anthrone Test Definition<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#objectives-of-anthrone-test\" title=\"Objectives of Anthrone Test\">Objectives of Anthrone Test<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#principle-of-anthrone-test\" title=\"Principle of Anthrone Test\">Principle of Anthrone Test<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#reaction\" title=\"Reaction\">Reaction<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#requirements\" title=\"Requirements\">Requirements<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#1-materials-required\" title=\"1. Materials required\">1. Materials required<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#2-reagents\" title=\"2. Reagents\">2. Reagents<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#procedure-of-anthrone-test\" title=\"Procedure of Anthrone Test\">Procedure of Anthrone Test<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#result-and-interpretation-of-anthrone-test\" title=\"Result and Interpretation of Anthrone Test\">Result and Interpretation of Anthrone Test<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#uses-of-anthrone-test\" title=\"Uses of Anthrone Test\">Uses of Anthrone Test<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#limitations-of-anthrone-test\" title=\"Limitations of Anthrone Test\">Limitations of Anthrone Test<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/microbenotes.com\/anthrone-test\/#references-and-sources\" title=\"References and Sources\">References and Sources<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"anthrone-test-definition\"><\/span><strong>Anthrone Test Definition<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>Anthrone test is a group test for <a href=\"https:\/\/microbenotes.com\/carbohydrates-structure-properties-classification-and-functions\/\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>carbohydrates<\/strong><\/a> that provides a rapid and convenient method for quantification of carbohydrates that are either free or bound to any <a href=\"https:\/\/microbenotes.com\/lipids-properties-structure-classification-and-functions\/\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>lipids<\/strong> <\/a>or <a href=\"https:\/\/microbenotes.com\/proteins-properties-structure-classification-and-functions\/\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>proteins<\/strong><\/a>.<\/p>\n<h3><span class=\"ez-toc-section\" id=\"objectives-of-anthrone-test\"><\/span><strong>Objectives of Anthrone Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>To detect the presence of carbohydrates in a given solution.<\/li>\n<li>To quantify the concentration of free and bound carbohydrates in a solution.<\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"principle-of-anthrone-test\"><\/span><strong>Principle of Anthrone Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p>If carbohydrate is present in the form of free carbohydrate as poly- or monosaccharide or bound as in a glycoprotein or a glycolipid, the concentrated acid in the Anthrone reagent first hydrolyses it into component monosaccharide. Similarly, the concentrated acid then catalyzes the dehydration of the monosaccharides to form furfural (from pentoses) or hydroxyl furfural (from hexoses). The furfural or hydroxyl furfural formed condenses with two molecules of naphthol from the Anthrone reagent to form a blue-green complex. The complex can then be quantified by measuring the absorbance of 620 nm wavelength in a spectrophotometer or in a red filter colorimeter.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-31555 size-full\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Anthrone-Test-Definition-Principle-Procedure-Result-Uses.jpeg\" alt=\"Anthrone Test\" width=\"900\" height=\"472\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Anthrone-Test-Definition-Principle-Procedure-Result-Uses.jpeg 900w, https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Anthrone-Test-Definition-Principle-Procedure-Result-Uses-300x157.jpeg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Anthrone-Test-Definition-Principle-Procedure-Result-Uses-768x403.jpeg 768w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/p>\n<p>Image Source: <a href=\"https:\/\/link.springer.com\/chapter\/10.1007\/978-1-4419-9785-2_5\" target=\"_blank\" rel=\"noopener noreferrer\">Rajan Katoch<\/a>.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"reaction\"><\/span><strong>Reaction<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><strong>1. Hydrolysis of polysaccharides to monosaccharide<\/strong><\/p>\n<p>Polysaccharide\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u2192\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Monosaccharides<\/p>\n<p><strong>2. Dehydration of monosaccharides to furfural<\/strong><\/p>\n<p>Monosaccharide\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u2192\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Furfural<\/p>\n<p><strong>3. Reaction of furfural with naphthol<\/strong><\/p>\n<p>Furfural + Anthrone reagent (naphthol)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u2192\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Blue-green complex<\/p>\n<h2><span class=\"ez-toc-section\" id=\"requirements\"><\/span><strong>Requirements<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"1-materials-required\"><\/span><strong>1. Materials required<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><strong>Equipment:<\/strong><\/p>\n<ul>\n<li>UV Spectrophotometer<\/li>\n<li>Vortex mixer<\/li>\n<li>Mantle heater\/Water Bath.<\/li>\n<\/ul>\n<p><strong>Chemicals\/Reagents:<\/strong><\/p>\n<ul>\n<li>Anthrone Reagent<\/li>\n<li>Glucose<\/li>\n<li>Other carbohydrates if desired<\/li>\n<li>Sample<\/li>\n<\/ul>\n<p><strong>Glasswares and other equipment:<\/strong><\/p>\n<ul>\n<li>Test tubes, Test tube stand, Pipettes, Beaker, Ice Test tube caps, Tissue paper, Wash bottle.<\/li>\n<\/ul>\n<h3><span class=\"ez-toc-section\" id=\"2-reagents\"><\/span><strong>2. Reagents<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li><strong>Anthrone reagent:<\/strong> 2g of Anthrone is dissolved in 1 liter of concentrated H<sub>2<\/sub>SO<sub>4<\/sub>. The freshly prepared reagent should be used for the assay<\/li>\n<li><strong>Glucose stock solution:<\/strong> 200\u00b5g glucose per mL distilled water stock solution of glucose is to be prepared from the stocked solution. Note: Other carbohydrates of the same concentration can be used as samples if desired.<\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"procedure-of-anthrone-test\"><\/span><strong>Procedure of Anthrone Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ol>\n<li>Pipette out different volumes (50 \u00b5l, 100 \u00b5l, and so on) of glucose solution from the supplied stock solution (200\u00b5g \/ml) into a series of test tubes and make up the volume to 1 mL with distilled water.<\/li>\n<li>Take a tube labeled as one as blank containing 1ml of just distilled water and the rest of the tubes labeled 2 to 9 for construction of a standard curve. Tubes 10-15 are for the unknown samples.<\/li>\n<li>Add 5 ml of the anthrone reagent To each tube and mix well by vortexing.<\/li>\n<li>Cool the tubes.<\/li>\n<li>Cover the tubes with caps on top and incubate at 90\u00b0C for 17 minutes or boiling water bath for 10 minutes.<\/li>\n<li>Cool the tubes to room temperature and measure the optical density of the solutions at 620 nm against a blank.<\/li>\n<li>Prepare a standard curve of absorbance against glucose concentration.<\/li>\n<li>Determine the amount of glucose in the unknown sample by plotting a standard curve of A620 on the Y-axis and concentration of Glucose on the X-axis.<\/li>\n<\/ol>\n<h2><span class=\"ez-toc-section\" id=\"result-and-interpretation-of-anthrone-test\"><\/span><strong>Result and Interpretation of Anthrone Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ul>\n<li>The presence of a blue-green complex indicates the presence of carbohydrates in the given solution.<\/li>\n<li>From the graph, we can determine the concentration of unknown samples.<\/li>\n<\/ul>\n<h3><span class=\"ez-toc-section\" id=\"uses-of-anthrone-test\"><\/span><strong>Uses of Anthrone Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>Anthrone test is used for the detection and quantification of carbohydrates in various samples like blood serum, milk, and its variation, etc.<\/li>\n<\/ul>\n<h3><span class=\"ez-toc-section\" id=\"limitations-of-anthrone-test\"><\/span><strong>Limitations of Anthrone Test<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li>Anthrone test gives a negative result in samples that are carbohydrates like D-glucose phenylosazone and D-glucose phenylosotriaole but do not form furfural or hydroxyfurfural in the dehydration step.<\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"references-and-sources\"><\/span><strong>References and Sources<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ul>\n<li><span style=\"font-size: 10pt;\">Am.(1950) Limitations of the Anthrone Test for Carbohydrates. Chem. Soc. 72. 8. <a href=\"https:\/\/doi.org\/10.1021\/ja01164a521\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.1021\/ja01164a521<\/a>.<\/span><\/li>\n<li><span style=\"font-size: 10pt;\">Tiwari A. (2015). Practical Biochemistry. LAP Lambert Academic Publishing.<\/span><\/li>\n<li><span style=\"font-size: 10pt;\">2% &#8211; https:\/\/allmedicalstuff.com\/molischs-test\/<\/span><\/li>\n<li><span style=\"font-size: 10pt;\">2% &#8211; http:\/\/www.jbc.org\/content\/208\/1\/55.full.pdf<\/span><\/li>\n<li><span style=\"font-size: 10pt;\">13% &#8211; https:\/\/www.iitg.ac.in\/biotech\/MTechLabProtocols\/Carbohydrate%20estimation%20by%20Anthrone.pdf<\/span><\/li>\n<li><span style=\"font-size: 10pt;\">1% &#8211; https:\/\/www.chem.purdue.edu\/courses\/chm224\/Lab-Experiments\/Experiment6_plus%20apparatus.pdf<\/span><\/li>\n<li><span style=\"font-size: 10pt;\">1% &#8211; https:\/\/en.wikipedia.org\/wiki\/Standard_curve<\/span><\/li>\n<li><span style=\"font-size: 10pt;\">1% &#8211; https:\/\/en.wikipedia.org\/wiki\/Molisch%27s_test<\/span><\/li>\n<li><span style=\"font-size: 10pt;\">1% &#8211; http:\/\/www.bcc.bas.bg\/BCC_Volumes\/Volume_48_Number_1_2016\/BCC-48-1-2016-3863-X-li%20%20Wang-109-113.pdf<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Anthrone Test Definition Anthrone test is a group test for carbohydrates that provides a rapid and convenient method for quantification of carbohydrates that are either free or bound to any &#8230; <a title=\"Anthrone Test- Definition, Principle, Procedure, Result, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/anthrone-test\/\" aria-label=\"Read more about Anthrone Test- Definition, Principle, Procedure, Result, Uses\">Read more<\/a><\/p>\n","protected":false},"author":11,"featured_media":31555,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1553],"tags":[5774,1826,5775],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2020\/11\/Anthrone-Test-Definition-Principle-Procedure-Result-Uses.jpeg","author":{"name":"Anupama Sapkota","avatar":"https:\/\/secure.gravatar.com\/avatar\/0e9c86afc1670c49efeb260138ec905b?s=96&d=mm&r=g"},"categories":[{"term_id":1553,"name":"Biochemistry","slug":"biochemistry","term_group":0,"term_taxonomy_id":1553,"taxonomy":"category","description":"<strong>Biochemistry is a branch of science that deals with the structure, composition, and metabolism of biomolecules found in different living organisms.<\/strong>\r\n<ul>\r\n \t<li>Biochemistry is also termed \u2018biological chemistry\u2019 as it deals with the chemistry of life that utilizes techniques from analytical, inorganic, and organic chemistry.<\/li>\r\n \t<li>Metabolic processes in living beings are composed of catabolic and anabolic processes. Both of these processes involve metabolic reactions involving biochemical compounds.<\/li>\r\n \t<li>Biochemistry is also used in techniques that help to understand the interactions, metabolism, and functions of biomolecules.<\/li>\r\n \t<li>Important biomolecules like proteins, carbohydrates, fats, nucleic acid, vitamins, etc. are essential structural and physiological components of living organisms.<\/li>\r\n \t<li>The study of the biochemical composition of living beings is important as it helps to understand the molecular mechanisms of various biochemical phenomena like respiration, photosynthesis, fermentation, etc.<\/li>\r\n \t<li>The findings of these studies, in turn, can be applied in industries, nutrition, medicine, and agriculture for different purposes.<\/li>\r\n \t<li>The rate of biochemical reactions, enzymes, and the concentration of substrates and products give essential information about the organism.<\/li>\r\n \t<li>Biochemical reactions form the basis of biochemical testing that is used for the identification and differentiation of microorganisms into different groups.<\/li>\r\n \t<li>Results of studies involving different biochemical tests can be applied for the production of pharmaceutical products that selectively inhibit certain metabolic processes.<\/li>\r\n \t<li>Techniques in biochemistry have been increasingly combined with techniques from other fields like agriculture, genetic, molecular biology, and biophysics.<\/li>\r\n \t<li>Biochemistry also allows the understanding of nutritional requirements for different living organisms.<\/li>\r\n \t<li>Biochemical compounds have been increasingly used even for the diagnosis of different diseases like diabetes.<\/li>\r\n \t<li>The determination of the genetic composition of living beings is also a part of biochemistry which helps in genetic studies and diagnosis of genetic diseases.<\/li>\r\n \t<li>Biochemical analysis of different biomolecules assists in the study relating to the origin and evolution of living beings.<\/li>\r\n \t<li>An understanding of different biochemical processes helps to understand the complexity of different groups of living beings and their relationship with one another.<\/li>\r\n<\/ul>","parent":0,"count":157,"filter":"raw","cat_ID":1553,"category_count":157,"category_description":"<strong>Biochemistry is a branch of science that deals with the structure, composition, and metabolism of biomolecules found in different living organisms.<\/strong>\r\n<ul>\r\n \t<li>Biochemistry is also termed \u2018biological chemistry\u2019 as it deals with the chemistry of life that utilizes techniques from analytical, inorganic, and organic chemistry.<\/li>\r\n \t<li>Metabolic processes in living beings are composed of catabolic and anabolic processes. Both of these processes involve metabolic reactions involving biochemical compounds.<\/li>\r\n \t<li>Biochemistry is also used in techniques that help to understand the interactions, metabolism, and functions of biomolecules.<\/li>\r\n \t<li>Important biomolecules like proteins, carbohydrates, fats, nucleic acid, vitamins, etc. are essential structural and physiological components of living organisms.<\/li>\r\n \t<li>The study of the biochemical composition of living beings is important as it helps to understand the molecular mechanisms of various biochemical phenomena like respiration, photosynthesis, fermentation, etc.<\/li>\r\n \t<li>The findings of these studies, in turn, can be applied in industries, nutrition, medicine, and agriculture for different purposes.<\/li>\r\n \t<li>The rate of biochemical reactions, enzymes, and the concentration of substrates and products give essential information about the organism.<\/li>\r\n \t<li>Biochemical reactions form the basis of biochemical testing that is used for the identification and differentiation of microorganisms into different groups.<\/li>\r\n \t<li>Results of studies involving different biochemical tests can be applied for the production of pharmaceutical products that selectively inhibit certain metabolic processes.<\/li>\r\n \t<li>Techniques in biochemistry have been increasingly combined with techniques from other fields like agriculture, genetic, molecular biology, and biophysics.<\/li>\r\n \t<li>Biochemistry also allows the understanding of nutritional requirements for different living organisms.<\/li>\r\n \t<li>Biochemical compounds have been increasingly used even for the diagnosis of different diseases like diabetes.<\/li>\r\n \t<li>The determination of the genetic composition of living beings is also a part of biochemistry which helps in genetic studies and diagnosis of genetic diseases.<\/li>\r\n \t<li>Biochemical analysis of different biomolecules assists in the study relating to the origin and evolution of living beings.<\/li>\r\n \t<li>An understanding of different biochemical processes helps to understand the complexity of different groups of living beings and their relationship with one another.<\/li>\r\n<\/ul>","cat_name":"Biochemistry","category_nicename":"biochemistry","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/31553"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=31553"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/31553\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/31555"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=31553"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=31553"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=31553"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}