{"id":2866,"date":"2022-01-02T20:01:00","date_gmt":"2022-01-02T14:16:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=2866"},"modified":"2022-01-08T02:52:19","modified_gmt":"2022-01-07T21:07:19","slug":"sorbitol-macconkey-agar","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/","title":{"rendered":"Sorbitol MacConkey Agar- Composition, Principle, Preparation, Results, Uses"},"content":{"rendered":"\n<p><span style=\"color: #000000;\">MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. It is selective and differential media for the detection of sorbitol-nonfermenting <em>Escherichia coli<\/em> serotype O157:H7 associated with hemorrhagic colitis. <em>E. coli<\/em> serotype O157:H7 is a human pathogen associated with hemorrhagic colitis that results from the action of a Shiga-like toxin (SLT). On standard lactose-containing MacConkey Agar, this strain is indistinguishable from other lactose-fermenting <em>E. coli<\/em>. Unlike most <em>E. coli<\/em> strains, <em>E. coli<\/em> O157:H7 ferments sorbitol slowly or not at all.\u00a0 Therefore, MacConkey Agar with Sorbitol permits recognition of <em>E. coli<\/em> O157:H7 in stool cultures. The addition of cefixime and tellurite is a more selective and differential medium designed to inhibit Proteus mirabilis, non-O157 <em>E. coli<\/em> strains, and other sorbitol-nonfermenting strains that need to be screened during the attempted isolation of <em>E. coli <\/em>O157:H7.<\/span><\/p>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/#composition-of-sorbitol-macconkey-agar\" title=\"Composition of Sorbitol MacConkey Agar\">Composition of Sorbitol MacConkey Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/#preparation-of-sorbitol-macconkey-agar\" title=\"Preparation of Sorbitol MacConkey Agar  \">Preparation of Sorbitol MacConkey Agar  <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/#principle-of-sorbitol-macconkey-agar\" title=\"Principle of Sorbitol MacConkey Agar \">Principle of Sorbitol MacConkey Agar <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/#result-and-interpretation-on-sorbitol-macconkey-agar\" title=\"Result and Interpretation on Sorbitol MacConkey Agar \">Result and Interpretation on Sorbitol MacConkey Agar <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/#uses-of-sorbitol-macconkey-agar\" title=\"Uses of Sorbitol MacConkey Agar  \">Uses of Sorbitol MacConkey Agar  <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/#limitations-of-sorbitol-macconkey-agar\" title=\"Limitations of Sorbitol MacConkey Agar\">Limitations of Sorbitol MacConkey Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/#references\" title=\"References \">References <\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-sorbitol-macconkey-agar\"><\/span><strong><span style=\"color: #000000;\">Composition <\/span>of Sorbitol MacConkey Agar<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Ingredients<\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Gm\/L<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Peptone<\/span><\/td><td><span style=\"color: #000000;\">17.0g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Proteose peptone<\/span><\/td><td><span style=\"color: #000000;\">3.0g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">D-Sorbitol<\/span><\/td><td><span style=\"color: #000000;\">10.0g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium Chloride<\/span><\/td><td><span style=\"color: #000000;\">5.0g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Bile Salts Mixture<\/span><\/td><td><span style=\"color: #000000;\">1.5g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Neutral Red<\/span><\/td><td><span style=\"color: #000000;\">0.03g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Crystal Violet<\/span><\/td><td><span style=\"color: #000000;\">0.001g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Agar<\/span><\/td><td><span style=\"color: #000000;\">13.5g<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><span style=\"color: #000000;\">Final pH 7.1 +\/- 0.2 at 25\u00baC<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-sorbitol-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Preparation <strong>of Sorbitol MacConkey Agar<\/strong>  <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Suspend 50.03 g of the powder in 1000ml distilled water. Mix thoroughly.<\/span><\/li><li><span style=\"color: #000000;\">Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.<\/span><\/li><li><span style=\"color: #000000;\">Autoclave at 121\u00b0C for 15 minutes.<\/span><\/li><li><span style=\"color: #000000;\">Cool to 45-50\u00b0C and pour into sterile Petri plates<\/span>.<\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-sorbitol-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Principle<\/strong><\/span> <strong>of Sorbitol MacConkey Agar<\/strong> <span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">MacConkey Sorbitol Agar is only slightly selective, since the concentration of bile salts, which inhibits gram-positive microorganisms, is low in comparison with other enteric plating media. Peptone and proteose peptone supply necessary nutrients like nitrogenous and carbonaceous compounds, long-chain amino acids, minerals, vitamins, and trace ingredients for the growth of organisms. Crystal violet present in the medium inhibits the growth of gram-positive bacteria, especially enterococci and staphylococci. Sodium chloride maintains osmotic equilibrium. Neutral red is an indicator. D-Sorbitol is a fermentable carbohydrate. Differentiation of enteric microorganisms is achieved by the combination of sorbitol and the neutral red indicator. The growth of <em>E.coli<\/em> O157:H7 on MacConkey Agar with Sorbitol shows colorless colonies and most of the fecal flora ferment sorbitol and appear pink. Colorless or pink to red colonies are produced depending upon the ability of the isolate to ferment the carbohydrate sorbitol.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"result-and-interpretation-on-sorbitol-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Result and Interpretation<\/strong><\/span> <strong>on Sorbitol MacConkey Agar<\/strong> <span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/07\/Sorbitol-MacConkey-Agar.jpg\" alt=\"Sorbitol MacConkey Agar\" class=\"wp-image-2867\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/07\/Sorbitol-MacConkey-Agar.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/07\/Sorbitol-MacConkey-Agar-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/07\/Sorbitol-MacConkey-Agar-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n\n<p><span style=\"color: #000000;\"><\/span><\/p>\n\n\n\n<p class=\"has-text-align-center\"><span style=\"color: #000000;\"><strong>Figure A: Escherichia coli ATCC 25922 and Figure B: Escherichia coli O157:H7.<br>Source: <a href=\"https:\/\/www.carlroth.com\/en\/en\/Chemicals\/A-Z-Chemicals\/S\/Sorbitol-MacConkey-Agar\/Sorbitol-MacConkey-Agar\/p\/000000030001d76700040023_en\" target=\"_blank\" rel=\"noopener noreferrer\">Carl Roth<\/a> and <a href=\"http:\/\/www.oxoid.com\/UK\/blue\/prod_detail\/prod_detail.asp?pr=CM0813&amp;c=UK&amp;lang=EN&amp;org=72&amp;img=CM0813&amp;sec=2\" target=\"_blank\" rel=\"noopener noreferrer\">Oxoid<\/a><\/strong><\/span><\/p>\n\n\n\n<ul><li><span style=\"color: #000000;\">After 18-24 hours of incubation, the plates should show isolated colonies in streaked areas and confluent growth in areas of heavy inoculation.<\/span><\/li><\/ul>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Sorbitol fermenters:<\/strong> &nbsp;pink to red colonies, some surrounded by zones of precipitated bile<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Sorbitol non-fermenters:<\/strong> colorless colonies.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-sorbitol-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Uses <strong>of Sorbitol MacConkey Agar<\/strong>  <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">It is used for isolation and detection of enteropathogenic <em>Escherichia coli<\/em> O157: H7 the primary serovar associated with hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS).<\/span><\/li><li><span style=\"color: #000000;\">It aids in the differentiation of the <em>E. coli<\/em> O157: H7 from other strains, especially lactose fermenters <em>E. coli.<\/em><\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations-of-sorbitol-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Limitations<\/strong><\/span> <strong>of Sorbitol MacConkey Agar<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">It has been reported that some Enterobacteriaceae and <em><a href=\"https:\/\/microbenotes.com\/pseudomonas-aeruginosa\/\" target=\"_blank\" rel=\"noreferrer noopener\">Pseudomonas aeruginosa<\/a><\/em> are inhibited on MacConkey Agar when incubated in a CO2-enriched atmosphere.<\/span><\/li><li><span style=\"color: #000000;\">Prolonged incubation of the culture may result in colonies of E. coli serotype O157:H7 losing their characteristic colorless appearance and also the color of sorbitol-positive colonies can fade, making them hard to distinguish from sorbitol-negative colonies.<\/span><\/li><li><span style=\"color: #000000;\">Prolonged incubation can result in fading of pink-colored sorbitol-positive colonies making interpretation more difficult. Upon prolonged incubation, some strains of <em>E. coli<\/em> O157:H7 can ferment sorbitol and produce pink-colored colonies.<\/span><\/li><li><span style=\"color: #000000;\">There are additional species of facultatively anaerobic gram-negative rods that do not ferment sorbitol.<\/span><\/li><li><span style=\"color: #000000;\">Although most non-O157:H7 <em> coli<\/em> ferment sorbitol, about 6% of the isolates do not. These atypical strains along with other sorbitol non-fermenting bacteria such as <em>Morganella<\/em> and <em>Hafnia<\/em> appear identical to O157:H7 colonies and therefore confirmatory tests may be necessary.<\/span><\/li><li><span style=\"color: #000000;\">There exist sorbitol negative strains of serotypes other than O157:H7 which may or may not produce toxins and clinical symptoms. <a href=\"https:\/\/microbenotes.com\/macconkey-agar\/\" target=\"_blank\" rel=\"noreferrer noopener\">MacConkey Agar<\/a> with Sorbitol does not differentiate between toxin-producing and nonproducing strains of <em>E. coli <\/em>O157.<\/span><\/li><li><span style=\"color: #000000;\">MacConkey Sorbitol Agar, however, should not be solely used to detect pathogenic E.coli O157: H7 strains as some nontoxic strains will also not ferment sorbitol. Gram staining, biochemical tests, and serological procedures should be performed to confirm findings.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Himedia<\/span><\/li><li><span style=\"color: #000000;\">Dalynn Biologicals<\/span><\/li><li><span style=\"color: #000000;\">Hardy Diagnostics<\/span><\/li><li><span style=\"color: #000000;\">Thermo Fisher Scientific Inc.<\/span><\/li><li><span style=\"color: #000000;\">Becton, Dickinson and Company<\/span><\/li><li><span style=\"color: #000000;\">Conda lab<\/span><\/li><li><span style=\"color: #000000;\">Tille P.M (2014)Bailey and Scott\u2019s diagnostic microbiology, Thirteen edition, Mosby, Inc., an affiliate of Elsevier Inc., 3251 Riverport Lane, St. Louis, Missouri 63043<\/span><\/li><li><span style=\"color: #000000;\">Ronald M. Atlas and James W. Snyder (2014). Handbook of media for clinical and public health microbiology. CRC Press. Taylor &amp; Francis Group, LLC. Page no.287.<\/span><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. It is selective and differential media for the detection of sorbitol-nonfermenting Escherichia coli serotype O157:H7 associated with &#8230; <a title=\"Sorbitol MacConkey Agar- Composition, Principle, Preparation, Results, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/sorbitol-macconkey-agar\/\" aria-label=\"Read more about Sorbitol MacConkey Agar- Composition, Principle, Preparation, Results, Uses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":2867,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[235,4837,4838,4839],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/07\/Sorbitol-MacConkey-Agar.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2866"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=2866"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2866\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/2867"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=2866"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=2866"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=2866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}