{"id":2615,"date":"2022-01-05T07:43:00","date_gmt":"2022-01-05T01:58:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=2615"},"modified":"2022-01-08T04:00:41","modified_gmt":"2022-01-07T22:15:41","slug":"peptone-yeast-extract-glucose-broth","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/","title":{"rendered":"Peptone Yeast Extract Glucose Broth"},"content":{"rendered":"\n<p><span style=\"color: #000000;\">Peptone Yeast Extract Broth Based Media are enriched nonselective media that are supplemented with hemin and vitamin K\u00a0to facilitate the recovery of the more fastidious organisms such as <em>Prevotella<\/em> spp, <em>Porphyromonas<\/em> spp, and the\u00a0<em>Bacteroides fragilis<\/em> group. With the addition of a specific biochemical, the Peptone Yeast Extract Broth Based Media are used to determine whether anaerobic bacteria will utilize the biochemical or if growth is stimulated or inhibited. Peptone Yeast Extract Glucose Broth is a nonselective broth media originally formulated by the Virginia Polytechnic Institute Anaerobe Laboratory (VPI) group to facilitate the recovery of more fastidious microorganisms and for use in chromatographic analysis of metabolic products from the <a href=\"https:\/\/microbenotes.com\/fermentation\/\" target=\"_blank\" rel=\"noreferrer noopener\">fermentation<\/a> of glucose. This chromatographic analysis is useful in the identification of clinically important anaerobic bacteria. The media is prepared, dispensed, and packaged under oxygen-free conditions in order to prevent the formation of oxidized products prior to use.<\/span><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/06\/Peptone-Yeast-Extract-Glucose-Broth.jpg\" alt=\"Peptone Yeast Extract Glucose Broth\" class=\"wp-image-2616\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/06\/Peptone-Yeast-Extract-Glucose-Broth.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/06\/Peptone-Yeast-Extract-Glucose-Broth-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/06\/Peptone-Yeast-Extract-Glucose-Broth-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#composition-of-peptone-yeast-extract-glucose-broth\" title=\"Composition of Peptone Yeast Extract Glucose Broth \">Composition of Peptone Yeast Extract Glucose Broth <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#principle-of-peptone-yeast-extract-glucose-broth\" title=\"Principle of Peptone Yeast Extract Glucose Broth  \">Principle of Peptone Yeast Extract Glucose Broth  <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#preparation-of-peptone-yeast-extract-glucose-broth\" title=\"Preparation of Peptone Yeast Extract Glucose Broth \">Preparation of Peptone Yeast Extract Glucose Broth <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#result-and-interpretation-on-peptone-yeast-extract-glucose-broth\" title=\"Result and Interpretation on Peptone Yeast Extract Glucose Broth  \">Result and Interpretation on Peptone Yeast Extract Glucose Broth  <\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#ph-measurements\" title=\"pH Measurements\">pH Measurements<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#interpretation-of-ph\" title=\"Interpretation of pH\">Interpretation of pH<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#uses-of-peptone-yeast-extract-glucose-broth\" title=\"Uses of Peptone Yeast Extract Glucose Broth  \">Uses of Peptone Yeast Extract Glucose Broth  <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#limitations\" title=\"Limitations\">Limitations<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/#references\" title=\"References\">References<\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-peptone-yeast-extract-glucose-broth\"><\/span><span style=\"color: #000000;\"><strong>Composition of Peptone Yeast Extract Glucose Broth <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Ingredients <\/strong><\/span><\/td><td><strong><span style=\"color: #000000;\">Grams\/L<\/span>iter<\/strong><\/td><\/tr><tr><td><span style=\"color: #000000;\">Pancreatic Digest of Casein<\/span><\/td><td><span style=\"color: #000000;\">20.0gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Yeast Extract<\/span><\/td><td><span style=\"color: #000000;\">10.0gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Glucose<\/span><\/td><td><span style=\"color: #000000;\">10.0gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">L-Cysteine<\/span><\/td><td><span style=\"color: #000000;\">0.5gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium Bicarbonate<\/span><\/td><td><span style=\"color: #000000;\">0.4gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium Chloride<\/span><\/td><td><span style=\"color: #000000;\">0.08gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Monopotassium Phosphate<\/span><\/td><td><span style=\"color: #000000;\">0.04gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Dipotassium Phosphate<\/span><\/td><td><span style=\"color: #000000;\">0.04gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Calcium Chloride, Anhydrous<\/span><\/td><td><span style=\"color: #000000;\">0.008gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Magnesium Sulfate, Anhydrous<\/span><\/td><td><span style=\"color: #000000;\">0.008gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Hemin Solution, 0.1%<\/span><\/td><td><span style=\"color: #000000;\">5.0ml<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Vitamin K Solution, 1%<\/span><\/td><td><span style=\"color: #000000;\">0.1ml<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><span style=\"color: #000000;\">Final pH 7.3 +\/- 0.5 at 25\u00baC.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-peptone-yeast-extract-glucose-broth\"><\/span><span style=\"color: #000000;\"><strong>Principle <span style=\"color: #000000;\"><strong>of Peptone Yeast Extract Glucose Broth<\/strong><\/span>  <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">Peptone Yeast Extract Glucose Broth contains a pancreatic digest of casein and yeast extract that supplies nitrogenous compounds and other nutrients required for the <\/span>growth of anaerobic bacteria. Sodium bicarbonate and dipotassium phosphate are buffers. Glucose serves as an energy source and L-cysteine is a reducing agent and maintains low oxygen potential which aid to stimulate the growth of anaerobic microorganisms. The growth factors hemin which supplies X factor and vitamin K are required for growth by many fastidious anaerobes and are also known to promote pigment production in certain anaerobic species. Sodium, potassium, calcium, and magnesium salts are pH stabilizers that<span style=\"color: #000000;\"> help to maintain osmotic balance and provide critical ions used in transport. The medium is prepared and processed in an atmosphere of nitrogen and hydrogen.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-peptone-yeast-extract-glucose-broth\"><\/span><span style=\"color: #000000;\"><strong>Preparation<\/strong><\/span> <span style=\"color: #000000;\"><strong>of Peptone Yeast Extract Glucose Broth<\/strong><\/span> <span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Add components to tap water and bring volume to 1.0 L. <\/span><\/li><li><span style=\"color: #000000;\">Mix thoroughly. <\/span><\/li><li><span style=\"color: #000000;\">Gently heat and bring to boiling. <\/span><\/li><li><span style=\"color: #000000;\">Distribute into tubes or flasks.<\/span><\/li><li><span style=\"color: #000000;\">Autoclave for 15 min at 15 psi pressure\u2013121\u00b0C. <\/span><\/li><li><span style=\"color: #000000;\">Pour into sterile Petri dishes or leave in tubes.<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"result-and-interpretation-on-peptone-yeast-extract-glucose-broth\"><\/span><span style=\"color: #000000;\"><strong>Result and Interpretation <span style=\"color: #000000;\"><strong>on Peptone Yeast Extract Glucose Broth<\/strong><\/span>  <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\"><strong>A positive result (inoculated medium):<\/strong> \u00a0Growth is evident by observation of slight turbidity (cloudiness) with smooth (sometimes stringy, granular, or flocculent) sediment.<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\"><strong>A negative result (uninoculated medium):<\/strong> No growth, no turbidity<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"ph-measurements\"><\/span><span style=\"color: #000000;\"><strong>pH Measurements<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<ul><li><span style=\"color: #000000;\">Once sufficient growth has been observed, the pH can be read directly from the culture tube using a pH meter and a long thin combination electrode.<\/span><\/li><\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"interpretation-of-ph\"><\/span><span style=\"color: #000000;\"><strong>Interpretation of pH<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<ul><li><span style=\"color: #000000;\">A pH value of 5.5 to 6.0 is considered a weak acid production.<\/span><\/li><li><span style=\"color: #000000;\">A pH value of 5.5 or less is strong acid production.<\/span><\/li><li><span style=\"color: #000000;\">A pH above 6.0 is indicative of no acid production.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-peptone-yeast-extract-glucose-broth\"><\/span><span style=\"color: #000000;\"><strong>Uses <span style=\"color: #000000;\"><strong>of Peptone Yeast Extract Glucose Broth<\/strong><\/span>  <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">It is used for the cultivation and biochemical identification of obligately anaerobic microorganisms.<\/span><\/li><li><span style=\"color: #000000;\">It is used to identify the short chain acid end products and for the determination of glucose fermentation by anaerobic bacteria for identification from clinical specimens.<\/span><\/li><li><span style=\"color: #000000;\">It is also suitable for the growth of anaerobic bacteria to be identified by gas-liquid chromatography (GLC) analysis.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations\"><\/span><span style=\"color: #000000;\"><strong>Limitations<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">Additional testing such as morphological, biochemical, and\/or serological tests should be performed for final identification to confirm the findings.<\/span><\/li><li><span style=\"color: #000000;\">T<\/span>his broth<span style=\"color: #000000;\"> will not provide complete information for the identification of bacterial isolates.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Himedia<\/span><\/li><li><span style=\"color: #000000;\">Thermofisher<\/span><\/li><li><span style=\"color: #000000;\">Becton, Dickinson and Company<\/span><\/li><li><span style=\"color: #000000;\">Hardy Diagnostic<\/span><\/li><li><span style=\"color: #000000;\">Anaerobe Systems<\/span><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Peptone Yeast Extract Broth Based Media are enriched nonselective media that are supplemented with hemin and vitamin K\u00a0to facilitate the recovery of the more fastidious organisms such as Prevotella spp, &#8230; <a title=\"Peptone Yeast Extract Glucose Broth\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/peptone-yeast-extract-glucose-broth\/\" aria-label=\"Read more about Peptone Yeast Extract Glucose Broth\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":2616,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[4735,4736,4737],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/06\/Peptone-Yeast-Extract-Glucose-Broth.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2615"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=2615"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2615\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/2616"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=2615"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=2615"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=2615"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}