{"id":2496,"date":"2022-01-01T18:28:00","date_gmt":"2022-01-01T12:43:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=2496"},"modified":"2022-01-08T04:18:18","modified_gmt":"2022-01-07T22:33:18","slug":"cystine-tryptic-agar","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/cystine-tryptic-agar\/","title":{"rendered":"Cystine Tryptic Agar- Composition, Principle, Preparation, Results, Uses"},"content":{"rendered":"\n<p><span style=\"color: #000000;\">Cystine tryptic agar (CTA), also known as cystine trypticase agar, was developed by Vera as a semi-solid growth medium used for the identification and maintenance of microorganisms for extended periods when stored at appropriate temperatures. It is used for the detection of bacterial motility and, with added carbohydrates<\/span>, it contributes to fermentation reactions and differentiation of fastidious microorganisms such as Neisseria, pneumococci, streptococci, and non-spore-forming<span style=\"color: #000000;\"> anaerobes.<\/span><\/p>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#composition-of-cystine-tryptic-agar\" title=\"Composition of Cystine Tryptic Agar\">Composition of Cystine Tryptic Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#principle-of-cystine-tryptic-agar\" title=\"Principle of Cystine Tryptic Agar\">Principle of Cystine Tryptic Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#preparation-of-cystine-tryptic-agar\" title=\"Preparation of Cystine Tryptic Agar\">Preparation of Cystine Tryptic Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#result-interpretation-of-cystine-tryptic-agar\" title=\"Result Interpretation of Cystine Tryptic Agar\">Result Interpretation of Cystine Tryptic Agar<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#fermentation\" title=\"Fermentation \">Fermentation <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#motility\" title=\"Motility \">Motility <\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#uses-of-cystine-tryptic-agar\" title=\"Uses of Cystine Tryptic Agar\">Uses of Cystine Tryptic Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#limitations-of-cystine-tryptic-agar\" title=\"Limitations of Cystine Tryptic Agar\">Limitations of Cystine Tryptic Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/#references\" title=\"References \">References <\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-cystine-tryptic-agar\"><\/span><strong><span style=\"color: #000000;\">Composition <\/span>of Cystine Tryptic Agar<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Ingredients <\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Gm\/L<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Pancreatic Digest of Casein<\/span><\/td><td><span style=\"color: #000000;\">20.0gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium Chloride<\/span><\/td><td><span style=\"color: #000000;\">5.0gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">L-Cystine<\/span><\/td><td><span style=\"color: #000000;\">0.5gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium Sulfite<\/span><\/td><td><span style=\"color: #000000;\">0.5gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Phenol Red<\/span><\/td><td><span style=\"color: #000000;\">17.0mg<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Agar<\/span><\/td><td><span style=\"color: #000000;\">3.5gm<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><span style=\"color: #000000;\">Final pH 7.5 +\/- 0.2 at 25\u00baC.<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Note:<\/strong> Additionally, CTA Media with carbohydrates contain 10.0gm of the specified carbohydrate.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-cystine-tryptic-agar\"><\/span><span style=\"color: #000000;\"><strong>Principle <\/strong><\/span><strong>of Cystine Tryptic Agar<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">The medium contains cystine and peptone to supply the nutrients necessary to support the growth of fastidious microorganisms. CTA Media supplemented with a 1% concentration of a specific carbohydrate are used to detect fermentation reactions. Carbohydrate fermentation is detected by a visible color change of the medium due to the incorporation of the pH indicator dye, phenol red. When the carbohydrate present is metabolized by the organism, organic acids are produced and the medium becomes acidified. The acid produced by carbohydrate fermentation causes a decrease in pH, causing a color shift in the medium from red-pink to yellow. However, the peptone present in the medium is also degraded by the bacteria present and yields substances that are alkaline in pH. The phenol red indicator changes from reddish-orange to yellow when the amount of acid produced by carbohydrate fermentation is greater than the alkaline end products of peptone degradation. The color change with phenol red occurs around pH 6.8, near the original pH of the medium. The addition of agar to the medium allows for the detection of motility along the stab line of inoculation. The motile organisms extend from the stab line and produce turbidity or cloudiness throughout the medium whereas the non-motile organisms grow only along the stab line and leave the surrounding medium clear.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-cystine-tryptic-agar\"><\/span><span style=\"color: #000000;\"><strong>Preparation <\/strong><\/span><strong>of Cystine Tryptic Agar<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Suspend 28.51 grams in 1000 ml distilled water.<\/span><\/li><li><span style=\"color: #000000;\">Heat to boiling to dissolve the medium completely.<\/span><\/li><li><span style=\"color: #000000;\">Dispense in tubes in 8-10 ml amounts.<\/span><\/li><li><span style=\"color: #000000;\">Sterilize by autoclaving at 121\u00b0C at 15 lbs pressure for 15 minutes.<\/span><\/li><li><span style=\"color: #000000;\">Cool to 50\u00b0C and add appropriate carbohydrate.<\/span><\/li><li><span style=\"color: #000000;\">Mix well and allow the medium in the test tube to cool in an upright position<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"result-interpretation-of-cystine-tryptic-agar\"><\/span><span style=\"color: #000000;\"><strong>Result Interpretation<\/strong><\/span> <strong>of Cystine Tryptic Agar<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/05\/Cystine-Tryptic-Agar.jpg\" alt=\"Cystine Tryptic Agar\" class=\"wp-image-2497\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/05\/Cystine-Tryptic-Agar.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/05\/Cystine-Tryptic-Agar-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/05\/Cystine-Tryptic-Agar-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n\n<p><span style=\"color: #000000;\"><\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Figure: HISS Medium (CTA + Serum + Sugar + Indicator (Phenol Red)). Image Source: <a style=\"color: #000000;\" href=\"https:\/\/slideplayer.com\/slide\/9849023\/32\/images\/11\/Neisseria+meningitides+Neisseria+gonorrhoeae.jpg\" target=\"_blank\" rel=\"noopener noreferrer\">slideplayer.com<\/a><\/strong><\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"fermentation\"><\/span><span style=\"color: #000000;\"><strong>Fermentation <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Positive reaction:<\/strong> development of a yellow color change in the inoculated area (stab line) of the medium.<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Negative reaction:<\/strong> a red-pink or deep red color in the medium. Growth with a deep red to orange color in the medium indicates that the carbohydrate has not been utilized and that peptone degradation has occurred.<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"motility\"><\/span><span style=\"color: #000000;\"><strong>Motility <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Positive test: <\/strong>Turbidity or cloudy growth extends from the line of inoculation.<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Negative test:<\/strong> Growth only along the stab line leaving the surroundings clear.<\/span><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"575\" height=\"623\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/05\/Cystine-Tryptic-Agar-Results.jpg\" alt=\"Cystine Tryptic Agar\" class=\"wp-image-2501\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/05\/Cystine-Tryptic-Agar-Results.jpg 575w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/05\/Cystine-Tryptic-Agar-Results-277x300.jpg 277w\" sizes=\"(max-width: 575px) 100vw, 575px\" \/><\/figure><\/div>\n\n\n\n<p><span style=\"color: #000000;\"><\/span><\/p>\n\n\n\n<p><strong><span style=\"color: #000000;\">Image Source: <a href=\"http:\/\/www.himedialabs.com\/intl\/en\/products\/Microbiology\/Dehydrated-Culture-Media-Biochemical-Identification-Media-Animal-based-Media\/Cystine-Tryptone-Agar-M159\" target=\"_blank\" rel=\"noopener noreferrer\">HiMedia<\/a><\/span><\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-cystine-tryptic-agar\"><\/span><span style=\"color: #000000;\"><strong>Uses <\/strong><\/span><strong>of Cystine Tryptic Agar<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">It is used for the identification and maintenance of the gonococcus and other bacteria.<\/span><\/li><li><span style=\"color: #000000;\">It is used for the determination of carbohydrate fermentation by fastidious microorganisms.<\/span><\/li><li><span style=\"color: #000000;\">It is also used for the detection of bacterial motility and additionally the base can serve as a holding medium for the maintenance of fastidious microorganisms.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations-of-cystine-tryptic-agar\"><\/span><span style=\"color: #000000;\"><strong>Limitations <\/strong><\/span><strong>of Cystine Tryptic Agar<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">It is recommended that biochemical, immunological, molecular, or mass spectrometry testing be performed on colonies from pure culture for complete identification.<\/span><\/li><li><span style=\"color: #000000;\">Only small amounts of acid may be produced by <i>Neisseria,<\/i>&nbsp;as the organisms utilize carbohydrates oxidatively.<\/span><\/li><li><span style=\"color: #000000;\">Prolonged incubation may lead to changes in pH indicator or abnormal lactose\/sucrose reactions with Neisseria pathogens.<\/span><\/li><li><span style=\"color: #000000;\">Aerobic incubation is necessary, as incubation in CO&nbsp;<sub>2&nbsp;<\/sub>may lead to erroneous results.<\/span><\/li><li><span style=\"color: #000000;\">CTA requires a heavy inoculum. Lack of sufficient inoculum may lead to erroneous results.<\/span><\/li><li><span style=\"color: #000000;\">Inoculating to the bottom of the tube and improper inoculation may lead to weak acid reactions, thus creating difficulty in test interpretation.<\/span><\/li><li><span style=\"color: #000000;\">Peptone utilization results in the production of alkaline by-products. Prolonged incubation may result in a reversion reaction where alkaline by-products mask the acid by-products formed from carbohydrate utilization.<\/span><\/li><li><span style=\"color: #000000;\">Because some strains of meningococci, primarily sulfonamide-resistant strains, do not produce acid from maltose, repeated subcultures to non-inhibitory media may be required to restore their maltose-utilizing capability.<\/span><\/li><li><span style=\"color: #000000;\">Some strains of gonococci require additional compounds not provided by the CTA Media formulations and will, therefore, not grow on CTA Media.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Hardy diagnostic<\/span><\/li><li><span style=\"color: #000000;\">Thermo fisher<\/span><\/li><li><span style=\"color: #000000;\">Becton, Dickinson and Company, <a style=\"color: #000000;\" href=\"https:\/\/www.bd.com\/europe\/regulatory\/Assets\/IFU\/Difco_BBL\/252310.pdf\" target=\"_blank\" rel=\"noopener\">https:\/\/www.bd.com\/europe\/regulatory\/Assets\/IFU\/Difco_BBL\/252310.pdf<\/a><\/span><\/li><li><span style=\"color: #000000;\">Northeast Laboratory Services<\/span><\/li><li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.nelabservices.com\/pdf\/tech-sheets\/cta-agar.pdf\" target=\"_blank\" rel=\"noopener\">https:\/\/www.nelabservices.com\/pdf\/tech-sheets\/cta-agar.pdf<\/a><\/span><\/li><li><span style=\"color: #000000;\">Ronald M. Atlas and James W. Snyder. Handbook of media for clinical and public health microbiology. CRC Press. 2014 by Taylor &amp; Francis Group, LLC.<\/span><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Cystine tryptic agar (CTA), also known as cystine trypticase agar, was developed by Vera as a semi-solid growth medium used for the identification and maintenance of microorganisms for extended periods &#8230; <a title=\"Cystine Tryptic Agar- Composition, Principle, Preparation, Results, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/cystine-tryptic-agar\/\" aria-label=\"Read more about Cystine Tryptic Agar- Composition, Principle, Preparation, Results, Uses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":2497,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[4699,4700,4704,4706,4702,4701,4703,4705,326],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/05\/Cystine-Tryptic-Agar.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2496"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=2496"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2496\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/2497"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=2496"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=2496"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=2496"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}