{"id":2280,"date":"2022-04-06T19:01:07","date_gmt":"2022-04-06T13:16:07","guid":{"rendered":"https:\/\/microbenotes.com\/?p=2280"},"modified":"2022-09-05T14:06:47","modified_gmt":"2022-09-05T08:21:47","slug":"biochemical-test-of-clostridium-botulinum","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/biochemical-test-of-clostridium-botulinum\/","title":{"rendered":"Biochemical Test of Clostridium botulinum"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/biochemical-test-of-clostridium-botulinum\/#type-a-and-proteolytic-strains-of-types-b-and-f\" title=\"(Type A and proteolytic strains of types B and F)\">(Type A and proteolytic strains of types B and F)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/biochemical-test-of-clostridium-botulinum\/#fermentation-of\" title=\"Fermentation of\">Fermentation of<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/biochemical-test-of-clostridium-botulinum\/#amino-acid-utilization\" title=\"Amino Acid Utilization\">Amino Acid Utilization<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/biochemical-test-of-clostridium-botulinum\/#enzymatic-reactions\" title=\"Enzymatic Reactions\">Enzymatic Reactions<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/biochemical-test-of-clostridium-botulinum\/#references\" title=\"References\">References<\/a><\/li><\/ul><\/nav><\/div>\n<h2><span class=\"ez-toc-section\" id=\"type-a-and-proteolytic-strains-of-types-b-and-f\"><\/span><span style=\"color: #000000;\"><strong>(Type A and proteolytic strains of types B and F)<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-2281\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/04\/Biochemical-Test-of-Clostridium-botulinum.jpg\" alt=\"Biochemical Test of Clostridium botulinum\" width=\"800\" height=\"420\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/04\/Biochemical-Test-of-Clostridium-botulinum.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/04\/Biochemical-Test-of-Clostridium-botulinum-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/04\/Biochemical-Test-of-Clostridium-botulinum-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\"><strong>Basic Characteristics<\/strong><\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\"><strong>Properties <\/strong><strong>(<em>Clostridium botulinum)<\/em><\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Ammonia Production<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">20% Bile<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Capsule<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Catalase<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Flagella<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Peritrichous<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Gelatin Hydrolysis<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Gram Staining<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Gram-positive<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">H<sub>2<\/sub>S<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Hemolysis<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve). \u03b2-hemolytic<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Indole<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Milk Digestion<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Mostly Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Meat Digestion<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Mostly Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Motility<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Mostly Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">6.5% NaCl<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Oxidase<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Shape<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Straight to slightly curved rods, 0.6\u20131.4 \u00d7 3.0\u201320.2 \u03bcm.<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Spore<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"522\">\n<h2><span class=\"ez-toc-section\" id=\"fermentation-of\"><\/span><span style=\"color: #000000;\"><strong>Fermentation of<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Arabinose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Cellobiose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Fructose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Galactose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Glucose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Glycogen<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Inulin<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Lactose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Maltose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Mannitol<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Mannose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Melibiose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Raffinose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Rhamnose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Ribose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Salicin<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Sorbitol<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Starch<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Sucrose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Trehalose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Xylose<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"522\">\n<h2><span class=\"ez-toc-section\" id=\"amino-acid-utilization\"><\/span><span style=\"color: #000000;\"><strong>Amino Acid Utilization<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Arginine<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Glycine<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Phenylalanine<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Proline<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Serine<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Tyrosine<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Tryptophan<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td colspan=\"2\" width=\"522\">\n<h2><span class=\"ez-toc-section\" id=\"enzymatic-reactions\"><\/span><span style=\"color: #000000;\"><strong>Enzymatic Reactions<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Casein Hydrolysis<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Esculin Hydrolysis<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Lecithinase<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Lipase<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Superoxide dismutase<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Positive (+ve)<\/span><\/td>\n<\/tr>\n<tr>\n<td width=\"228\"><span style=\"color: #000000;\">Tryptophan Deaminase<\/span><\/td>\n<td width=\"294\"><span style=\"color: #000000;\">Negative (-ve)<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ol>\n<li><span style=\"color: #000000;\">Bergey, D H, William B. Whitman, Vos P. De, George M. Garrity, and D Jones.\u00a0Bergey&#8217;s Manual of Systematic Bacteriology: Vol. 3. New York: Springer, 2009. Print.<\/span><\/li>\n<li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.vetbact.org\/?artid=202\" target=\"_blank\" rel=\"noopener\">https:\/\/www.vetbact.org\/?artid=202<\/a><\/span><\/li>\n<li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.fda.gov\/food\/foodscienceresearch\/laboratorymethods\/ucm070879.htm\" target=\"_blank\" rel=\"noopener\">https:\/\/www.fda.gov\/food\/foodscienceresearch\/laboratorymethods\/ucm070879.htm<\/a><\/span><\/li>\n<li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"http:\/\/www.tgw1916.net\/Clostridium\/botulinum.html\" target=\"_blank\" rel=\"noopener\">http:\/\/www.tgw1916.net\/Clostridium\/botulinum.html<\/a><\/span><\/li>\n<li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.who.int\/csr\/delibepidemics\/clostridiumbotulism.pdf\" target=\"_blank\" rel=\"noopener\">https:\/\/www.who.int\/csr\/delibepidemics\/clostridiumbotulism.pdf<\/a><\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>(Type A and proteolytic strains of types B and F) Basic Characteristics Properties (Clostridium botulinum) Ammonia Production Positive (+ve) 20% Bile Negative (-ve) Capsule Negative (-ve) Catalase Negative (-ve) Flagella &#8230; <a title=\"Biochemical Test of Clostridium botulinum\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/biochemical-test-of-clostridium-botulinum\/\" aria-label=\"Read more about Biochemical Test of Clostridium botulinum\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":2281,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[702],"tags":[272,4373,303,4370,4371,4372],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/04\/Biochemical-Test-of-Clostridium-botulinum.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":702,"name":"Biochemical Test","slug":"biochemical-test","term_group":0,"term_taxonomy_id":702,"taxonomy":"category","description":"<strong>Biochemical tests are the tests that are performed on different bacteria for their identification on the basis of their biochemical activities towards different biochemical compounds.<\/strong>\r\n<ul>\r\n \t<li>Biochemical tests are one of the traditional methods for the identification of microorganisms, usually performed with phenotypic identification.<\/li>\r\n \t<li>For many years these methods were employed extensively, and they continue to be used nowadays, especially in some laboratory routines where a particular type of microorganism has to be identified rapidly.<\/li>\r\n \t<li>The ability of microorganisms to utilize certain biomolecules, resulting in useful organic compounds for themselves forms the basis of various biochemical tests.<\/li>\r\n \t<li>Biochemical tests are of different types, where the identification or distinction between different microorganisms is made on various bases.<\/li>\r\n \t<li>One of the traditional methods commonly used is a simple visual detection of the growth of the organism in the presence of essential nutrients by increased turbidity in the liquid medium.<\/li>\r\n \t<li>In other tests, however, the results are based on the change in color of the medium as a result of the change in the pH of the medium.<\/li>\r\n \t<li>Microorganisms can be classified into different groups on the basis of their reaction to such tests. Some tests even allow the distinction of microorganisms to the species level.<\/li>\r\n \t<li>Biochemical tests are thus, essential as they are inexpensive and relatively simple to perform.<\/li>\r\n \t<li>The physiology of bacteria and other microorganisms differs from one another, which allows for the differentiation of such microorganisms.<\/li>\r\n \t<li>Biochemical tests, however, have some disadvantages. Despite being inexpensive and allowing both quantitative and qualitative information about the diversity of microorganisms present in a sample, these methods are laborious and time-consuming, and results are only observed after several days.<\/li>\r\n \t<li>In some cases, false positives are obtained, especially when considering similar microbial species.<\/li>\r\n<\/ul>","parent":0,"count":114,"filter":"raw","cat_ID":702,"category_count":114,"category_description":"<strong>Biochemical tests are the tests that are performed on different bacteria for their identification on the basis of their biochemical activities towards different biochemical compounds.<\/strong>\r\n<ul>\r\n \t<li>Biochemical tests are one of the traditional methods for the identification of microorganisms, usually performed with phenotypic identification.<\/li>\r\n \t<li>For many years these methods were employed extensively, and they continue to be used nowadays, especially in some laboratory routines where a particular type of microorganism has to be identified rapidly.<\/li>\r\n \t<li>The ability of microorganisms to utilize certain biomolecules, resulting in useful organic compounds for themselves forms the basis of various biochemical tests.<\/li>\r\n \t<li>Biochemical tests are of different types, where the identification or distinction between different microorganisms is made on various bases.<\/li>\r\n \t<li>One of the traditional methods commonly used is a simple visual detection of the growth of the organism in the presence of essential nutrients by increased turbidity in the liquid medium.<\/li>\r\n \t<li>In other tests, however, the results are based on the change in color of the medium as a result of the change in the pH of the medium.<\/li>\r\n \t<li>Microorganisms can be classified into different groups on the basis of their reaction to such tests. Some tests even allow the distinction of microorganisms to the species level.<\/li>\r\n \t<li>Biochemical tests are thus, essential as they are inexpensive and relatively simple to perform.<\/li>\r\n \t<li>The physiology of bacteria and other microorganisms differs from one another, which allows for the differentiation of such microorganisms.<\/li>\r\n \t<li>Biochemical tests, however, have some disadvantages. Despite being inexpensive and allowing both quantitative and qualitative information about the diversity of microorganisms present in a sample, these methods are laborious and time-consuming, and results are only observed after several days.<\/li>\r\n \t<li>In some cases, false positives are obtained, especially when considering similar microbial species.<\/li>\r\n<\/ul>","cat_name":"Biochemical Test","category_nicename":"biochemical-test","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2280"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=2280"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2280\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/2281"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=2280"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=2280"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=2280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}