{"id":2149,"date":"2022-01-02T20:58:00","date_gmt":"2022-01-02T15:13:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=2149"},"modified":"2022-01-08T01:21:22","modified_gmt":"2022-01-07T19:36:22","slug":"deoxycholate-citrate-agar-dca","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/","title":{"rendered":"Deoxycholate Citrate Agar (DCA)- Composition, Principle, Preparation, Results, Uses"},"content":{"rendered":"\n<ul><li><span style=\"color: #000000;\">Deoxycholate Citrate Agar is a modification of Leifson formula and is recommended for the isolation of <em>Salmonella <\/em>and <em>Shigella <\/em>spp.<\/span><\/li><li><span style=\"color: #000000;\">This medium is similar to deoxycholate agar in comparison but is moderately more selective for enteric pathogens owing to increased concentrations of both citrate and deoxycholate salts.<\/span><\/li><li><span style=\"color: #000000;\">Sodium deoxycholate at pH 7.3 to 7.5 is inhibitory for <a href=\"https:\/\/microbenotes.com\/gram-positive-bacteria\/\" target=\"_blank\" rel=\"noreferrer noopener\">gram-positive bacteria<\/a>. Citrate salts, in the concentration included in the formulation, are inhibitory to gram-positive bacteria and most other normal intestinal organisms.<\/span><\/li><li><span style=\"color: #000000;\">It is thus a selective and differential medium,\u00a0used commonly for the isolation of enteric pathogens.<\/span><\/li><\/ul>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Deoxycholate-Citrate-Agar-DCA.jpg\" alt=\"Deoxycholate Citrate Agar (DCA)\" class=\"wp-image-2150\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Deoxycholate-Citrate-Agar-DCA.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Deoxycholate-Citrate-Agar-DCA-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Deoxycholate-Citrate-Agar-DCA-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/#composition-of-deoxycholate-citrate-agar-dca\" title=\"Composition of Deoxycholate Citrate Agar (DCA)\">Composition of Deoxycholate Citrate Agar (DCA)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/#principle-of-deoxycholate-citrate-agar-dca\" title=\"Principle of Deoxycholate Citrate Agar (DCA)\">Principle of Deoxycholate Citrate Agar (DCA)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/#preparation-and-method-of-use-of-deoxycholate-citrate-agar-dca\" title=\"Preparation and Method of Use of Deoxycholate Citrate Agar (DCA)\">Preparation and Method of Use of Deoxycholate Citrate Agar (DCA)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/#result-interpretation-on-deoxycholate-citrate-agar-dca\" title=\"Result Interpretation on Deoxycholate Citrate Agar (DCA)\">Result Interpretation on Deoxycholate Citrate Agar (DCA)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/#uses-of-deoxycholate-citrate-agar-dca\" title=\"Uses of Deoxycholate Citrate Agar (DCA)\">Uses of Deoxycholate Citrate Agar (DCA)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/#limitations-of-deoxycholate-citrate-agar-dca\" title=\"Limitations of Deoxycholate Citrate Agar (DCA)\">Limitations of Deoxycholate Citrate Agar (DCA)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/#references\" title=\"References \">References <\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-deoxycholate-citrate-agar-dca\"><\/span><span style=\"color: #000000;\"><strong>Composition of Deoxycholate Citrate Agar (DCA)<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Ingredients<\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Gms\/liter<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">HI solids<\/span><\/td><td><span style=\"color: #000000;\">10.00<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Proteose peptone<\/span><\/td><td><span style=\"color: #000000;\">10.00<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Lactose<\/span><\/td><td><span style=\"color: #000000;\">10.00<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium deoxycholate<\/span><\/td><td><span style=\"color: #000000;\">5.000<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Neutral red<\/span><\/td><td><span style=\"color: #000000;\">0.020<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium citrate<\/span><\/td><td><span style=\"color: #000000;\">20.00<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Ferric ammonium citrate<\/span><\/td><td><span style=\"color: #000000;\">2.000<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Agar<\/span><\/td><td><span style=\"color: #000000;\">13.50<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><span style=\"color: #000000;\">Final pH (at 25\u00b0C) 7.5\u00b10.2<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-deoxycholate-citrate-agar-dca\"><\/span><span style=\"color: #000000;\"><strong>Principle of Deoxycholate Citrate Agar (DCA)<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">HI solids is a source of carbon and nitrogen and result in the inhibition of coliforms.<\/span><\/li><li><span style=\"color: #000000;\">Proteose peptone provides carbon, nitrogen, vitamins and minerals.<\/span><\/li><li><span style=\"color: #000000;\">Coliform bacteria and gram-positive bacteria are inhibited or greatly suppressed due to sodium deoxycholate, sodium citrate, and ferric ammonium citrate.<\/span><\/li><li><span style=\"color: #000000;\">Dipotassium phosphate buffers the medium.<\/span><\/li><li><span style=\"color: #000000;\">Lactose helps in differentiating enteric bacilli, as lactose fermenters produce red colonies while lactose non-fermenters produce colourless colonies.<\/span><\/li><li><span style=\"color: #000000;\">Coliform bacteria, if present form pink colonies on this medium.<\/span><\/li><li><span style=\"color: #000000;\">The degradation of lactose causes acidification of the medium surrounding the relevant colonies and the pH indicator neutral red changes its color to red. These colonies usually are also surrounded by a turbid zone of precipitated deoxycholic acid due to acidification of the medium.<\/span><\/li><li><span style=\"color: #000000;\">Sodium deoxycholate combines with neutral red in an acidic environment, causing the dye to go out of the solution with the subsequent precipitation of deoxycholate.<\/span><\/li><li><span style=\"color: #000000;\">The reduction of ferric ammonium citrate to iron sulfide is indicated by the formation of black iron sulfide.<\/span><\/li><li><span style=\"color: #000000;\">Salmonella and Shigella species do not ferment lactose but Salmonella may produce H2S, forming colorless colonies with or without black centers.<\/span><\/li><li><span style=\"color: #000000;\">Citrate and iron (Fe) combination has a strong hydrolyzing effect on agar when the medium is heated, producing a soft and unelastic agar.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-and-method-of-use-of-deoxycholate-citrate-agar-dca\"><\/span><span style=\"color: #000000;\"><strong>Preparation and Method of Use of Deoxycholate Citrate Agar (DCA)<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Suspend 70.52 grams in 1000 ml of distilled water.<\/span><\/li><li><span style=\"color: #000000;\">Heat to boiling to dissolve the medium completely.<\/span><\/li><\/ol>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Note:<\/strong> DO NOT AUTOCLAVE. Avoid excessive heating as it is detrimental to the medium.<\/span><\/p>\n\n\n\n<ol start=\"3\"><li><span style=\"color: #000000;\">Cool to 45-50\u00b0C. Mix well and pour into sterile Petri plates.<\/span><\/li><li><span style=\"color: #000000;\">Dry the agar surface before use.<\/span><\/li><li><span style=\"color: #000000;\">Inoculate the medium heavily with feces or rectal swabs, spreading part of the original inoculum in order to obtain well-separated colonies on some portion of the plate.<\/span><\/li><li><span style=\"color: #000000;\">Incubate for 18-24 hours at 35\u00b0C.<\/span><\/li><li><span style=\"color: #000000;\">If organisms are late developers or if no non-lactose fermenters are observed, incubate for a further 24 hours.<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"result-interpretation-on-deoxycholate-citrate-agar-dca\"><\/span><span style=\"color: #000000;\"><strong>Result Interpretation on Deoxycholate Citrate Agar (DCA)<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">Lactose non-fermenters produce transparent, colorless to light pink or tan-colored colonies with or without black centers.<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\">Lactose fermenters produce a red colony with or without a bile precipitate.<\/span><\/p>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Organisms<\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Growth<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Escherichia coli<\/em><\/span><\/td><td><span style=\"color: #000000;\">Poor growth; pink with bile precipitate negative reaction for H<sub>2<\/sub>S<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Salmonella<\/em> Enteritidis<\/span><\/td><td><span style=\"color: #000000;\">Good-luxuriant growth; colorless; positive reaction for H<sub>2<\/sub>S, black centered colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Salmonella<\/em> Typhimurium<\/span><\/td><td><span style=\"color: #000000;\">Good-luxuriant growth; colorless; positive reaction for H<sub>2<\/sub>S, black centered colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Shigella flexneri<\/em><\/span><\/td><td><span style=\"color: #000000;\">Good growth; colorless<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Salmonella <\/em>Abony<\/span><\/td><td><span style=\"color: #000000;\">Good-luxuriant growth; colorless; positive reaction for H<sub>2<\/sub>S, black centered colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Shigella&nbsp;sonnei<\/em><\/span><\/td><td><span style=\"color: #000000;\">Colonies are smooth and initially colorless, becoming pale pink on further incubation due to late lactose fermentation<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Enterobacter\/Klebsiella <\/em>spp.<\/span><\/td><td><span style=\"color: #000000;\">Large, pale mucoid colonies with the pink center<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-deoxycholate-citrate-agar-dca\"><\/span><span style=\"color: #000000;\"><strong>Uses of Deoxycholate Citrate Agar (DCA)<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">Deoxycholate Citrate Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting.<\/span><\/li><li><span style=\"color: #000000;\">It is particularly useful for the isolation of organisms that cause bacillary dysentery, <em>Salmonella&nbsp;<\/em>strains that cause&nbsp;food poisoning&nbsp;and&nbsp;<em>Salmonella <\/em>Paratyphi.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations-of-deoxycholate-citrate-agar-dca\"><\/span><span style=\"color: #000000;\"><strong>Limitations of Deoxycholate Citrate Agar (DCA)<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">Suspected pathogens must be subcultured on a less inhibitory medium prior to identification.&nbsp;&nbsp;<\/span><\/li><li><span style=\"color: #000000;\">As there are many bacteria that also look like <em>Salmonella<\/em> on DCA, it is widely recommended that more selective agars are used for the identification of&nbsp;<em>Salmonella<\/em>, namely xylose lysine deoxycholate (XLD) agar.&nbsp;<\/span><\/li><li><span style=\"color: #000000;\">This growth medium is heat-sensitive and should be poured and cooled as soon as possible after the addition of the deoxycholate, otherwise, it tends to become very soft and difficult to handle.<\/span><\/li><li><span style=\"color: #000000;\">For the routine examination of stool and urine specimens, it is suggested that other media such as MacConkey Agar, Bismuth Sulphite Agar etc. be used in conjunction with this medium.<\/span><\/li><li><span style=\"color: #000000;\">Further biochemical identification is required for confirmation of species.<\/span><\/li><li><span style=\"color: #000000;\">Due to nutritional variations, some organisms may show poor growth.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"http:\/\/himedialabs.com\/TD\/M065.pdf\" target=\"_blank\" rel=\"noopener\">http:\/\/himedialabs.com\/TD\/M065.pdf<\/a><\/span><\/li><li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.usbio.net\/culture-media\" target=\"_blank\" rel=\"noopener\">https:\/\/www.usbio.net\/culture-media<\/a><\/span><\/li><li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.bd.com\/europe\/regulatory\/Assets\/IFU\/Difco_BBL\/227410.pdf\" target=\"_blank\" rel=\"noopener\">https:\/\/www.bd.com\/europe\/regulatory\/Assets\/IFU\/Difco_BBL\/227410.pdf<\/a><\/span><\/li><li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"http:\/\/foodsafety.neogen.com\/uk\/deoxycholate-citrate-agar\" target=\"_blank\" rel=\"noopener\">http:\/\/foodsafety.neogen.com\/uk\/deoxycholate-citrate-agar<\/a><\/span><\/li><li><span style=\"color: #000000;\">Speck M. (Ed.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd ed., APHA, Washington, D.C<\/span><\/li><li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.thomassci.com\/Laboratory-Supplies\/Microbiological-Media\/_\/Deoxycholate-Citrate-Agar-Modified-Hynes\" target=\"_blank\" rel=\"noopener\">https:\/\/www.thomassci.com\/Laboratory-Supplies\/Microbiological-Media\/_\/Deoxycholate-Citrate-Agar-Modified-Hynes<\/a><\/span><\/li><li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.sigmaaldrich.com\/catalog\/product\/sial\/d7809?lang=en&amp;region=US\" target=\"_blank\" rel=\"noopener\">https:\/\/www.sigmaaldrich.com\/catalog\/product\/sial\/d7809?lang=en&amp;region=US<\/a><\/span><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Deoxycholate Citrate Agar is a modification of Leifson formula and is recommended for the isolation of Salmonella and Shigella spp. This medium is similar to deoxycholate agar in comparison but &#8230; <a title=\"Deoxycholate Citrate Agar (DCA)- Composition, Principle, Preparation, Results, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/deoxycholate-citrate-agar-dca\/\" aria-label=\"Read more about Deoxycholate Citrate Agar (DCA)- Composition, Principle, Preparation, Results, Uses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":2150,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[4186,4214,4212,4215,4213,4211,4216,4222,4219,4218,4217,4220,4221],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Deoxycholate-Citrate-Agar-DCA.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2149"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=2149"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2149\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/2150"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=2149"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=2149"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=2149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}