{"id":2117,"date":"2022-01-04T17:37:00","date_gmt":"2022-01-04T11:52:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=2117"},"modified":"2022-01-08T03:22:10","modified_gmt":"2022-01-07T21:37:10","slug":"columbia-agar-with-5-sheep-blood","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/","title":{"rendered":"Columbia Agar with 5% Sheep Blood- Composition, Principle, Preparation, Results, Uses"},"content":{"rendered":"\n<p><span style=\"color: #000000;\">Columbia Agar with 5% Sheep blood is a <\/span>highly nutritious general-purpose medium for the isolation and cultivation of the non-fastidious and fastidious microorganisms from various clinical specimens and non-clinical specimens of public health importance.&nbsp; Columbia Blood Agar was first described in 1966 by Ellner et al who incorporated animal-derived<span style=\"color: #000000;\"> peptone, enzymatic digests of casein, and enriched medium by addition of the defibrinated sheep blood into one medium.&nbsp;It was found to be an improved blood agar, promoting both luxuriant and rapid growth, improved pigment production, typical colony morphology, and sharply defined hemolytic reactions.<\/span><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Columbia-Agar-with-5-Sheep-Blood.jpg\" alt=\"Columbia Agar with 5% Sheep Blood\" class=\"wp-image-2118\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Columbia-Agar-with-5-Sheep-Blood.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Columbia-Agar-with-5-Sheep-Blood-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Columbia-Agar-with-5-Sheep-Blood-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#composition-of-columbia-agar-with-5-sheep-blood\" title=\"Composition of Columbia Agar with 5% Sheep Blood \">Composition of Columbia Agar with 5% Sheep Blood <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#principle-of-columbia-agar-with-5-sheep-blood\" title=\"Principle of Columbia Agar with 5% Sheep Blood \">Principle of Columbia Agar with 5% Sheep Blood <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#preparation-of-columbia-agar-with-5-sheep-blood\" title=\"Preparation of Columbia Agar with 5% Sheep Blood\">Preparation of Columbia Agar with 5% Sheep Blood<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#preparation-of-columbia-agar-base\" title=\"Preparation of Columbia Agar Base\">Preparation of Columbia Agar Base<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#preparation-of-columbia-agar-with-5-sheep-blood-2\" title=\"Preparation of Columbia Agar with 5% Sheep Blood\">Preparation of Columbia Agar with 5% Sheep Blood<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#results-interpretation-of-columbia-agar-with-5-sheep-blood\" title=\"Results Interpretation of Columbia Agar with 5% Sheep Blood \">Results Interpretation of Columbia Agar with 5% Sheep Blood <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#uses-of-columbia-agar-with-5-sheep-blood\" title=\"Uses of Columbia Agar with 5% Sheep Blood \">Uses of Columbia Agar with 5% Sheep Blood <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#limitations-of-columbia-agar-with-5-sheep-blood\" title=\"Limitations of Columbia Agar with 5% Sheep Blood \">Limitations of Columbia Agar with 5% Sheep Blood <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/#references\" title=\"References \">References <\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-columbia-agar-with-5-sheep-blood\"><\/span><span style=\"color: #000000;\"><strong>Composition of Columbia Agar with 5% Sheep Blood <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\">Pancreatic Digest of Casein<\/span><\/td><td><span style=\"color: #000000;\">12.0 g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Peptic Digest of Animal Tissue<\/span><\/td><td><span style=\"color: #000000;\">5.0 g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Yeast Extract<\/span><\/td><td><span style=\"color: #000000;\">3.0 g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Beef Extract<\/span><\/td><td><span style=\"color: #000000;\">3.0 g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Corn Starch<\/span><\/td><td><span style=\"color: #000000;\">1.0 g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium Chloride<\/span><\/td><td><span style=\"color: #000000;\">5.0 g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Agar<\/span><\/td><td><span style=\"color: #000000;\">13.5 g<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sheep Blood, Defibrinated<\/span><\/td><td><span style=\"color: #000000;\">5 % (50ml)<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><span style=\"color: #000000;\">pH 7.3 \u00b1 0.2 at 25\u00b0C<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-columbia-agar-with-5-sheep-blood\"><\/span><span style=\"color: #000000;\"><strong>Principle of Columbia Agar with 5% Sheep Blood <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">The medium contains peptones that supply nitrogen, carbon, vitamins,<\/span> and trace elements necessary for the growth of the bacterium. Yeast extract act as the supplier of the vitamin B complex. Corn starch serves as the energy source for organisms and also neutralizes toxic metabolites by absorbing toxic by-products contained in the specimen. Sodium chloride is a source of <span style=\"color: #000000;\">essential electrolytes and maintains osmotic equilibrium. Sheep blood is added to demonstrate hemolytic reactions and supplies the X factor (heme) necessary for the growth of many pathogenic species. On this medium, colonies tend to be larger and growth is more luxuriant than on media containing other blood agar bases.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-columbia-agar-with-5-sheep-blood\"><\/span><span style=\"color: #000000;\"><strong>Preparation of Columbia Agar with 5% Sheep Blood<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-columbia-agar-base\"><\/span><span style=\"color: #000000;\"><strong>Preparation of Columbia Agar Base<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<ol><li><span style=\"color: #000000;\">Add components to distilled\/ deionized water and bring volume to 1.0L. <\/span><\/li><li><span style=\"color: #000000;\">Mix thoroughly. <\/span><\/li><li><span style=\"color: #000000;\">Gently heat until boiling.<\/span><\/li><li><span style=\"color: #000000;\">Autoclave for 15 min at 15 psi pressure\u2013121\u00b0C.<\/span><\/li><li><span style=\"color: #000000;\">Cool to 45\u00b0\u201350\u00b0C.<\/span><\/li><\/ol>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-columbia-agar-with-5-sheep-blood-2\"><\/span><span style=\"color: #000000;\"><strong>Preparation of Columbia Agar with 5% Sheep Blood<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<ol><li><span style=\"color: #000000;\">To 950.0mL of cooled, sterile Columbia agar base, aseptically add 50.0mL of sterile, defibrinated sheep blood. <\/span><\/li><li><span style=\"color: #000000;\">Mix thoroughly. <\/span><\/li><li><span style=\"color: #000000;\">Pour into sterile Petri dishes or distribute into sterile tubes.<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"results-interpretation-of-columbia-agar-with-5-sheep-blood\"><\/span><span style=\"color: #000000;\"><strong>Results Interpretation of Columbia Agar with 5% Sheep Blood <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Organisms <\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Colony characteristics<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Streptococci (non-group D)<\/span><\/td><td><span style=\"color: #000000;\">Small, white to grayish.<\/span>  <p><span style=\"color: #000000;\">Beta or alpha hemolysis<\/span><\/p><\/td><\/tr><tr><td><span style=\"color: #000000;\">Enterococci (Group D)<\/span><\/td><td><span style=\"color: #000000;\">Small, but larger than group A streptococci, grayish.<\/span> <p>&nbsp;<span style=\"color: #000000;\">Alpha (rarely beta) hemolysis<\/span><\/p><\/td><\/tr><tr><td><span style=\"color: #000000;\">Staphylococci<\/span><\/td><td><span style=\"color: #000000;\">Large, white to gray or cream to yellow, with or without hemolysis<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Corynebacteria<\/span><\/td><td><span style=\"color: #000000;\">Small to large, white to gray or yellow, with or without hemolysis<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Listeria monocytogenes<\/em><\/span><\/td><td><span style=\"color: #000000;\">Small to medium-sized, grayish, with weak beta hemolysis<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Enterobacteriaceae<\/span><\/td><td><span style=\"color: #000000;\">Medium-sized to large, grey colonies, with or without hemolysis<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Candida<\/em> species<\/span><\/td><td><span style=\"color: #000000;\">Small, white colonies<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-columbia-agar-with-5-sheep-blood\"><\/span><span style=\"color: #000000;\"><strong>Uses of Columbia Agar with 5% Sheep Blood <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">Columbia Blood Agar is recommended as a frequently used primary isolation medium for isolation of non-fastidious and fastidious microorganisms from clinical specimens.<\/span><\/li><li><span style=\"color: #000000;\">It is a primary isolation medium on which most microorganisms, such as Enterobacteriaceae, <em>Pseudomonas, <\/em>and other non-fermenting Gram-negative rods, streptococci, enterococci, staphylococci, coryneforms, <em><a href=\"https:\/\/microbenotes.com\/candida-albicans\/\" target=\"_blank\" rel=\"noreferrer noopener\">Candida<\/a><\/em> species, and many others will grow.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations-of-columbia-agar-with-5-sheep-blood\"><\/span><span style=\"color: #000000;\"><strong>Limitations of Columbia Agar with 5% Sheep Blood <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul><li><span style=\"color: #000000;\">It is recommended that biochemical, immunological, molecular and mass spectrometry testing be performed on colonies from pure culture for complete identification.<\/span><\/li><li><span style=\"color: #000000;\">The medium lacks V factor (nicotinamide adenine dinucleotide, NAD) since sheep blood contains NADase which destroys the NAD. Hence, <em>Haemophilus influenzae<\/em> which requires both the X and V factors, will not grow on this medium.<\/span><\/li><li><span style=\"color: #000000;\"><em>Neisseria gonorrhoeae<\/em> does not grow well on this medium.<\/span><\/li><li><span style=\"color: #000000;\">The medium is not suitable for the isolation and growth of <em>Mycobacterium, Legionella, Bordetella<\/em> and other organisms with highly specific nutritive requirements.<\/span><\/li><li><span style=\"color: #000000;\">Due to the rather high carbohydrate (starch) content in the Columbia Agar Base, beta-hemolytic streptococci may exhibit alpha rather than beta hemolytic reactions or may exhibit weak hemolytic reactions on media based on this formulation.<\/span><\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Ronald M. Atlas and James W. Snyder (2014). Handbook of media for clinical and public health microbiology. CRC Press. Taylor &amp; Francis Group, LLC. Page no.148-149.<\/span><\/li><li><span style=\"color: #000000;\">Becton Dickinson<\/span><\/li><li><span style=\"color: #000000;\">Himedia<\/span><\/li><li><span style=\"color: #000000;\">Hardy Diagnostics<\/span><\/li><li><span style=\"color: #000000;\">Thermo Fisher<\/span><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Columbia Agar with 5% Sheep blood is a highly nutritious general-purpose medium for the isolation and cultivation of the non-fastidious and fastidious microorganisms from various clinical specimens and non-clinical specimens &#8230; <a title=\"Columbia Agar with 5% Sheep Blood- Composition, Principle, Preparation, Results, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/columbia-agar-with-5-sheep-blood\/\" aria-label=\"Read more about Columbia Agar with 5% Sheep Blood- Composition, Principle, Preparation, Results, Uses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":2118,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[227,4186,717,718,4184,4187,4185],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Columbia-Agar-with-5-Sheep-Blood.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2117"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=2117"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2117\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/2118"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=2117"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=2117"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=2117"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}