{"id":2017,"date":"2022-01-02T12:11:00","date_gmt":"2022-01-02T06:26:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=2017"},"modified":"2022-01-08T01:18:59","modified_gmt":"2022-01-07T19:33:59","slug":"cystine-lactose-electrolyte-deficient-cled-agar","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/","title":{"rendered":"CLED Agar- Composition, Principle, Preparation, Results, Uses"},"content":{"rendered":"\n<p><span style=\"color: #000000;\">Cystine-Lactose-Electrolyte-Deficient (CLED) medium, first described by Sandys\u00a0and later modified by Mackey and Sandys,\u00a0is generally used for diagnostic routine urinary bacteriology as a non-selective medium capable of supporting the growth of most urinary pathogens.\u00a0CLED Agar is a differential medium used for the isolation and enumeration of bacteria from urine. The medium supports the growth of all urinary potential pathogens and provides distinct colony morphology. It supports the growth of urinary pathogens and contaminants and gives good colonial differentiation without the spread of\u00a0<em>Proteus<\/em>\u00a0species due to its lack of electrolytes.<\/span><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Cystine-Lactose-Electrolyte-Deficient-CLED-Agar.jpg\" alt=\"Cystine Lactose Electrolyte Deficient (CLED) Agar\" class=\"wp-image-2018\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Cystine-Lactose-Electrolyte-Deficient-CLED-Agar.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Cystine-Lactose-Electrolyte-Deficient-CLED-Agar-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Cystine-Lactose-Electrolyte-Deficient-CLED-Agar-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/#composition-of-cled-agar\" title=\"Composition of CLED Agar\">Composition of CLED Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/#principle-of-cled-agar\" title=\"Principle of CLED Agar \">Principle of CLED Agar <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/#preparation-of-cled-agar\" title=\"Preparation of CLED Agar\">Preparation of CLED Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/#colony-characteristics-of-various-organisms-on-cled-agar\" title=\"Colony characteristics of various organisms on CLED Agar\">Colony characteristics of various organisms on CLED Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/#uses-of-cled-agar\" title=\"Uses of CLED Agar\">Uses of CLED Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/#limitations-of-cled-agar\" title=\"Limitations of CLED Agar\">Limitations of CLED Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/#references\" title=\"References\">References<\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-cled-agar\"><\/span><span style=\"color: #000000;\"><strong>Composition of CLED Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Constituents<\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; gm\/Litre<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Lactose<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 10.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Enzymatic Digest of Gelatin<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 4.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Enzymatic Digest of Casein<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 4.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Beef extract<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 3.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">L-Cystine<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 0.128<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Bromothymol Blue<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 0.02<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Agar<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 15.0<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><span style=\"color: #000000;\">pH 7.3 +\/- 0.2<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-cled-agar\"><\/span><span style=\"color: #000000;\"><strong>Principle of CLED Agar <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">The CLED comprises of enzymatic digest of Casein, an <\/span>enzymatic digest of gelatin, and beef extract which provide the\u00a0nitrogen, vitamins, minerals, and amino acids essential for growth. L-cystine supports the growth of the cysteine-dependent dwarf coliform colony. Lactose is a\u00a0fermentable carbohydrate that provides carbon and energy. \u00a0Organisms capable of fermenting lactose will lower the pH and change the color of the medium from green to yellow. The color change is indicated by the presence of the <span style=\"color: #000000;\">pH indicator Bromthymol Blue. Agar present in the medium is the\u00a0solidifying agent.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-cled-agar\"><\/span><span style=\"color: #000000;\"><strong>Preparation of CLED Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Suspend 36 gram of the medium in one liter of distilled water.<\/span><\/li><li><span style=\"color: #000000;\">Mix well and heat with frequent agitation and boil for one minute until complete to dissolution.<\/span><\/li><li><span style=\"color: #000000;\">Autoclave at 121\u00b0C for 15 minutes.<\/span><\/li><li><span style=\"color: #000000;\">Cool to 50\u00b0C, mix well and dispense into plates. When the medium is solidified, invert the plates to avoid excess moisture. The prepared medium should be stored at 8-15\u00b0C.<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"colony-characteristics-of-various-organisms-on-cled-agar\"><\/span><span style=\"color: #000000;\"><strong>Colony characteristics of various organisms on CLED Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">Various cultural responses are shown by different microorganisms on CLED Agar at the appropriate atmosphere and temperature after 18 \u2013 24 hours incubation are as follows:<\/span><\/p>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Organism<\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Colony Morphology<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em>Escherichia coli<\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">Large, elevated, yellow, opaque colonies with a center more intense yellow; yellowish medium<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em>Klebsiella species<\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">extremely mucoid colonies varying in color from yellow to whitish-blue. yellowish medium.<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em>Proteus species<\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">translucent blue colonies are usually smaller than Escherichia coli. blue-green to blue medium.<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em>Salmonella species<\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">flat blue colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em><a href=\"https:\/\/microbenotes.com\/pseudomonas-aeruginosa\/\" target=\"_blank\" rel=\"noreferrer noopener nofollow\">Pseudomonas aeruginosa<\/a><\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">green colonies with a typical matte surface and rough periphery. \u201cSweet\u201d odor. Blue-green agar<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em>Enterococcus faecalis<\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">yellow colonies about 0.5 mm in diameter. yellow medium<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em>Staphylococcus aureus<\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">deep yellow colonies about 0.75 mm diameter, uniform in color. yellow medium<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em>Corynebacteria<\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">very small grey colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><strong><em>Lactobacilli<\/em><\/strong><\/span><\/td><td><span style=\"color: #000000;\">similar to corynebacteria but with a rougher surface<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-cled-agar\"><\/span><span style=\"color: #000000;\"><strong>Uses of CLED Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">It is used for routine urine culture and uses for growth and enumeration of urinary tract organisms.<\/span><\/li><li><span style=\"color: #000000;\">It helps in the differentiation of lactose fermenter and lactose non-fermenter.<\/span><\/li><li><span style=\"color: #000000;\">It is suitable for the isolation and counting of many aerobically growing microorganisms, such as Enterobacteriaceae, <em>Pseudomonas<\/em>, and other non-fermenting Gram negative rods, enterococci, staphylococci, <em>Candida<\/em> species, and many others from urine specimens.<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations-of-cled-agar\"><\/span><span style=\"color: #000000;\"><strong>Limitations of CLED Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Streptococci and other organisms requiring blood or serum for growth may only be insufficiently recovered on this medium or may need extended incubation. Therefore, the specimen should also be cultivated onto a blood agar plate if such organisms are expected.<\/span><\/li><li><span style=\"color: #000000;\">Genitourinary pathogens such as <em>Neisseria gonorrhoeae<\/em>, <em>Gardnerella vaginalis<\/em>, <em>Chlamydia<\/em>, <em>Ureaplasma<\/em>, or other fastidious organisms do not grow on this medium.<\/span><\/li><li><span style=\"color: #000000;\">Serological tests using pure cultures are necessary for complete identification.<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Conda Lab<\/span><\/li><li><span style=\"color: #000000;\">Himedia<\/span><\/li><li><span style=\"color: #000000;\"><a style=\"color: #000000;\" href=\"https:\/\/www.bd.com\/resource.aspx?IDX=8967\" target=\"_blank\" rel=\"noopener\">https:\/\/www.bd.com\/resource.aspx?IDX=8967<\/a><\/span><\/li><li><span style=\"color: #000000;\">Fallon D, Andrews N, Frodsham D, <a style=\"color: #000000;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Gee%20B%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=12101200\" target=\"_blank\" rel=\"noopener\">Gee<\/a> B,&nbsp;<a style=\"color: #000000;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Howe%20S%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=12101200\" target=\"_blank\" rel=\"noopener\"> Howe<\/a> S,&nbsp;<a style=\"color: #000000;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Iliffe%20A%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=12101200\" target=\"_blank\" rel=\"noopener\">Iliffe<\/a> A,&nbsp;<a style=\"color: #000000;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Nye%20KJ%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=12101200\" target=\"_blank\" rel=\"noopener\">Nye<\/a> KJand&nbsp;<a style=\"color: #000000;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=Warren%20RE%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=12101200\" target=\"_blank\" rel=\"noopener\">R E Warren<\/a> RE (2002). A comparison of the performance of cystine lactose electrolyte deficient (CLED) agar with Oxoid chromogenic urinary tract infection (CUTI) medium for the isolation and presumptive identification of organisms from urine.&nbsp;Journal of Clinical Pathology 55(7):524-529.<\/span><\/li><li><span style=\"color: #000000;\">Cheesbrough M., District Laboratory Practice in Tropical Countries: Part 2:.Cambridge University Press.<\/span><\/li><li><span style=\"color: #000000;\">Ronald M. Atlas and James W. Snyder (2014). Handbook of media for clinical and public health microbiology. CRC Press. Taylor &amp; Francis Group, LLC<\/span><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Cystine-Lactose-Electrolyte-Deficient (CLED) medium, first described by Sandys\u00a0and later modified by Mackey and Sandys,\u00a0is generally used for diagnostic routine urinary bacteriology as a non-selective medium capable of supporting the growth of &#8230; <a title=\"CLED Agar- Composition, Principle, Preparation, Results, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/cystine-lactose-electrolyte-deficient-cled-agar\/\" aria-label=\"Read more about CLED Agar- Composition, Principle, Preparation, Results, Uses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":2018,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[4114,4115,4120,4117,4116,4118,4119,4112,4113],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2019\/03\/Cystine-Lactose-Electrolyte-Deficient-CLED-Agar.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2017"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=2017"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/2017\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/2018"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=2017"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=2017"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=2017"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}