{"id":1272,"date":"2022-01-06T20:07:00","date_gmt":"2022-01-06T14:22:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=1272"},"modified":"2023-01-13T13:23:31","modified_gmt":"2023-01-13T07:38:31","slug":"macconkey-agar","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/macconkey-agar\/","title":{"rendered":"MacConkey Agar- Composition, Principle, Preparation, Results, Uses"},"content":{"rendered":"\n<p><span style=\"color: #000000;\"><strong>MacConkey agar<\/strong> was the first solid differential media to be formulated\u00a0which\u00a0was developed in<\/span> the 20th century by Alfred Theodore MacConkey.\u00a0MacConkey Agar is the earliest selective and differential medium for the <span style=\"color: #000000;\">cultivation of coliform organisms. It is used for the isolation and differentiation of non-fastidious gram-negative rods, particularly members of the family Enterobacteriaceae and the genus\u00a0<strong><em><a href=\"https:\/\/microbenotes.com\/pseudomonas-aeruginosa\/\">Pseudomonas<\/a><\/em><\/strong>.<\/span><\/p>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/macconkey-agar\/#composition-of-macconkey-agar\" title=\"Composition of MacConkey Agar\">Composition of MacConkey Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/macconkey-agar\/#principle-of-macconkey-agar\" title=\"Principle of MacConkey Agar \">Principle of MacConkey Agar <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/macconkey-agar\/#preparation-of-macconkey-agar\" title=\"Preparation of MacConkey Agar \">Preparation of MacConkey Agar <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/macconkey-agar\/#result-interpretation-on-macconkey-agar\" title=\"Result Interpretation on MacConkey Agar\">Result Interpretation on MacConkey Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/macconkey-agar\/#uses-of-macconkey-agar\" title=\"Uses of MacConkey Agar \">Uses of MacConkey Agar <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/macconkey-agar\/#limitations-of-macconkey-agar\" title=\"Limitations of MacConkey Agar \">Limitations of MacConkey Agar <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/macconkey-agar\/#references\" title=\"References\">References<\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Composition of MacConkey Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Ingredients<\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Amount<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Peptone (Pancreatic digest of gelatin)&nbsp;<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;17 gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Proteose peptone&nbsp;(meat and casein)<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;3 gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Lactose&nbsp;monohydrate&nbsp;<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;10 gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Bile salts<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;1.5 gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium chloride<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;5 gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Neutral red<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;0.03 gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Crystal Violet<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;0.001 gm<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Agar<\/span><\/td><td><span style=\"color: #000000;\">&nbsp;13.5 gm<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Principle of MacConkey Agar <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">Pancreatic digest of gelatin and peptones (meat and casein) provide the essential nutrients, vitamins,<\/span> and nitrogenous factors required for the growth of microorganisms. Lactose monohydrate is the fermentable source of carbohydrates<span style=\"color: #000000;\">. The selective action of this medium is attributed to crystal violet and bile salts, which are inhibitory to most species of gram-positive bacteria. Sodium chloride maintains the osmotic balance in the medium.<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\">Lactose fermenting strains grow <\/span>red or pink. The red color is due to the production of acid from lactose, absorption of neutral red, and a subsequent color change of the dye when the pH of the medium falls below 6.8. Non-lactose fermenting bacteria such as&nbsp;<em>Salmonella,&nbsp;Proteus,&nbsp;Pseudomonas aeruginosa, <\/em>and<em> Shigella<\/em> cannot utilize lactose<span style=\"color: #000000;\"> and will use peptone instead. This forms ammonia, which raises the pH of the agar, and leads to the formation of white or colorless colonies formed in the plate and the agar surrounding the bacteria remains relatively transparent. These colonies can also look golden to brown with dark centers.<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;\">Lactose can be replaced in the medium by other sugars and the replacement sugars can be used to test the abilities of gram-negative bacteria to ferment the replacement sugars. For example, sorbitol can be used as a replacement sugar in MacConkey agar to assist in the isolation and differentiation of enteropathogenic&nbsp;<em>E. coli&nbsp;<\/em>serotypes such as <em>E. coli<\/em> O157:H7 by the presence of white circular colonies that are non-sorbitol fermenting.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Preparation of MacConkey Agar <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol>\n<li><span style=\"color: #000000;\">Suspend 49.53 grams of dehydrated medium in 1000 ml of distilled water.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Heat to boiling to dissolve the medium&nbsp;completely.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Sterilize by autoclaving at 15 lbs pressure (121\u00b0C) for 15 minutes.&nbsp;<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Cool to 45\u00b0C -50\u00b0C.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Mix well before pouring into sterile Petri plates.<\/span><\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"result-interpretation-on-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Result Interpretation on MacConkey Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/09\/Result-Interpretation-on-MacConkey-Agar.jpg\" alt=\"Result Interpretation on MacConkey Agar\" class=\"wp-image-1273\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/09\/Result-Interpretation-on-MacConkey-Agar.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/09\/Result-Interpretation-on-MacConkey-Agar-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/09\/Result-Interpretation-on-MacConkey-Agar-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Organisms<\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Growth results <\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Escherichia coli <\/em><\/span><\/td><td><span style=\"color: #000000;\">Pink to rose-red colonies (may be surrounded by a zone of precipitated bile)<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Enterobacter, Klebsiella <\/em><\/span><\/td><td><span style=\"color: #000000;\">Mucoid, pink colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Proteus <\/em><\/span><\/td><td><span style=\"color: #000000;\">Colorless colonies, swarming growth<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Salmonella, Shigella <\/em><\/span><\/td><td><span style=\"color: #000000;\">Colorless colonies, or sometimes medium color:&nbsp; orange to amber<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Pseudomonas <\/em><\/span><\/td><td><span style=\"color: #000000;\">Irregular, colorless to pink colonies<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Uses of MacConkey Agar <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol>\n<li><span style=\"color: #000000;\">MacConkey agar is used for the isolation of gram-negative enteric bacteria.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">It is used in the differentiation of lactose fermenting from lactose non-fermenting gram-negative bacteria.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">It is use in microbiological examination of food stuffs and for direct plating \/ inoculation of water samples for coliform counts.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">It is used for the examination of milk and dairy products.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">British pharmacopoeia has recommended this medium for the subculture and identification of <em>Escherichia coli.<\/em><\/span><\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations-of-macconkey-agar\"><\/span><span style=\"color: #000000;\"><strong>Limitations of MacConkey Agar <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol>\n<li><span style=\"color: #000000;\">The colonial characteristics describe give presumptive identification only of the isolated organisms. It is necessary to subculture and carryout other confirmation tests like biochemical tests for final identification.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Some strains may be encountered that grow poorly or fail to grow on this medium.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Incubation of MacConkey Agar plates under increased CO2&nbsp;has been reported to reduce growth and&nbsp;recovery of a number of strains of Gram-negative bacilli.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Some strains of Proteus may swarm on this medium.<\/span><\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol>\n<li><span style=\"color: #000000;\">https:\/\/catalog.hardydiagnostics.com\/cp_prod\/Content\/hugo\/MacConkeyAgar.htm<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">http:\/\/www.himedialabs.com\/TD\/M081B.pdf<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">http:\/\/www.himedialabs.com\/TD\/M008S.pdf<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">https:\/\/www.teknova.com\/category-s\/504.htm<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">http:\/\/www.bd.com\/resource.aspx?IDX=8978<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">https:\/\/microbeonline.com\/macconkey-agar-mac-composition-preparation-uses-and-colony-characteristics\/<\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>MacConkey agar was the first solid differential media to be formulated\u00a0which\u00a0was developed in the 20th century by Alfred Theodore MacConkey.\u00a0MacConkey Agar is the earliest selective and differential medium for the &#8230; <a title=\"MacConkey Agar- Composition, Principle, Preparation, Results, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/macconkey-agar\/\" aria-label=\"Read more about MacConkey Agar- Composition, Principle, Preparation, Results, Uses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":1273,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[450,2513,2514,235,2506,2507,2512,2509,2508,2510,2511,2515,2518,2516,2517],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/09\/Result-Interpretation-on-MacConkey-Agar.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/1272"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=1272"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/1272\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/1273"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=1272"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=1272"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=1272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}