{"id":1142,"date":"2022-01-05T20:24:00","date_gmt":"2022-01-05T14:39:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=1142"},"modified":"2023-01-13T23:31:12","modified_gmt":"2023-01-13T17:46:12","slug":"mannitol-salt-agar-msa","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/","title":{"rendered":"Mannitol Salt Agar (MSA)- Composition, Principle, Preparation, Results, Uses"},"content":{"rendered":"\n<p><span style=\"color: #000000;\">Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of\u00a0<strong><em><a href=\"https:\/\/microbenotes.com\/staphylococcus-aureus\/\" data-type=\"post\" data-id=\"32229\">Staphylococcus aureus<\/a><\/em><\/strong>\u00a0from clinical and non-clinical specimens. It is recommended for the detection and enumeration of coagulase-positive Staphylococci in milk, food, and other specimens and encourages the growth of a group of certain bacteria while inhibiting the growth of others.<\/span><\/p>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/#principle-of-mannitol-salt-agar-msa\" title=\"Principle of&nbsp;Mannitol Salt Agar (MSA) \">Principle of&nbsp;Mannitol Salt Agar (MSA) <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/#composition-of-mannitol-salt-agar-msa\" title=\"Composition of&nbsp;Mannitol Salt Agar (MSA) \">Composition of&nbsp;Mannitol Salt Agar (MSA) <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/#preparation-of-mannitol-salt-agar-msa\" title=\"Preparation of&nbsp;Mannitol Salt Agar (MSA) \">Preparation of&nbsp;Mannitol Salt Agar (MSA) <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/#results-on-mannitol-salt-agar-msa\" title=\"Results&nbsp;on Mannitol Salt Agar (MSA)\">Results&nbsp;on Mannitol Salt Agar (MSA)<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/#uses-of-mannitol-salt-agar-msa\" title=\"Uses of&nbsp;Mannitol Salt Agar (MSA) \">Uses of&nbsp;Mannitol Salt Agar (MSA) <\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/#limitations\" title=\"Limitations\">Limitations<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/#references\" title=\"References \">References <\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-mannitol-salt-agar-msa\"><\/span><span style=\"color: #000000;\"><strong>Principle of&nbsp;Mannitol Salt Agar (MSA) <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">Mannitol Salt Agar contains beef extract and proteose peptone, which makes it very nutritious as they provide essential growth factors and trace nutrients such as nitrogen, vitamins, minerals, and amino acids essential for growth. The medium contains a 7.5% concentration of&nbsp;<strong>sodium chloride<\/strong>&nbsp;which results in the partial or complete inhibition of bacterial organisms other than staphylococci.&nbsp;<strong>Mannitol<\/strong>&nbsp;is the fermentable carbohydrate source, fermentation of which leads to acid production. Staphylococcus aureus grows on this medium and ferments mannitol to produce yellow colonies. Most coagulase-negative species of Staphylococci and Micrococci do not ferment mannitol and grow as small red colonies. The color of the colonies and the medium is due to the reactivity of phenol red (indicator) to the pH of the medium. Phenol red is red at pH 8.4 and phenol red is yellow at pH 6.8. <strong>Agar<\/strong>&nbsp;is the solidifying agent. In addition, 5% v\/v&nbsp;<strong>Egg Yolk Emulsion<\/strong>&nbsp;can be added to a medium which enables the detection of lipase activity of staphylococci along with mannitol fermentation. The salt clears the egg yolk emulsion and lipase production is detected as a yellow opaque zone around the colonies.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-mannitol-salt-agar-msa\"><\/span><span style=\"color: #000000;\"><strong>Composition of&nbsp;Mannitol Salt Agar (MSA) <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Ingredients <\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Gms\/Litre<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Proteose peptone<\/span><\/td><td><span style=\"color: #000000;\">10.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium chloride<\/span><\/td><td><span style=\"color: #000000;\">75.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">D- mannitol<\/span><\/td><td><span style=\"color: #000000;\">10.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Beef extract<\/span><\/td><td><span style=\"color: #000000;\">1.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Phenol red<\/span><\/td><td><span style=\"color: #000000;\">0.025<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Agar<\/span><\/td><td><span style=\"color: #000000;\">15.0<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><span style=\"color: #000000;\">Final pH 7.4 +\/- 0.2 at 25\u00baC.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-mannitol-salt-agar-msa\"><\/span><span style=\"color: #000000;\"><strong>Preparation of&nbsp;Mannitol Salt Agar (MSA) <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol>\n<li><span style=\"color: #000000;\">Suspend 111 grams of Mannitol Salt Agar in 1000 ml of distilled water.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Boil to dissolve the medium completely.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Sterilize by autoclaving at 15 lbs. pressure (121\u00b0C) for 15 minutes.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">If desired, sterile Egg Yolk Emulsion (E7899) can be added to a final concentration of 5% v\/v after autoclaving.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Pour cooled Mannitol Salt Agar into sterile petri dishes and allow to cool to room temperature.<\/span><\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"results-on-mannitol-salt-agar-msa\"><\/span><span style=\"color: #000000;\"><strong>Results&nbsp;on Mannitol Salt Agar (MSA)<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Results-on-Mannitol-Salt-Agar-MSA.jpg\" alt=\"Results\u00a0on Mannitol Salt Agar (MSA)\" class=\"wp-image-1143\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Results-on-Mannitol-Salt-Agar-MSA.jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Results-on-Mannitol-Salt-Agar-MSA-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Results-on-Mannitol-Salt-Agar-MSA-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Organisms<\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Results <\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Staphylococcus aureus <\/em><\/span><\/td><td><span style=\"color: #000000;\">Yellow colonies surrounded by the yellow zone<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Staphylococcus epidermidis <\/em><\/span><\/td><td><span style=\"color: #000000;\">Pink or Red colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Micrococci<\/span><\/td><td><span style=\"color: #000000;\">Red colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Escherichia coli<\/em><\/span><\/td><td><span style=\"color: #000000;\">No growth<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-mannitol-salt-agar-msa\"><\/span><span style=\"color: #000000;\"><strong>Uses of&nbsp;Mannitol Salt Agar (MSA) <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol>\n<li><span style=\"color: #000000;\">It is used for the selective isolation and differentiation of&nbsp;<em>Staphylococcus aureus<\/em>&nbsp;from clinical samples.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">It is also used for the enumeration of staphylococci in food and dairy products.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">This medium is also included in the Bacteriological Analytical Manual for cosmetics testing.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">It is also used in the bacteriological examination of swimming pool water, spas and drinking water using membrane filtration.<\/span><\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations\"><\/span><span style=\"color: #000000;\"><strong>Limitations<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol>\n<li><span style=\"color: #000000;\">Several&nbsp;<em>Staphylococcus<\/em>&nbsp;species other than&nbsp;<em>aureus<\/em>&nbsp;are mannitol positive and produce yellow colonies surrounded by yellow zones on this medium (e.g.&nbsp;<em>S. capitis, S. xylosus, S. cohnii, S. sciuri, S. simulans<\/em>, and other species). Therefore, further biochemical tests are necessary for the identification of S. aureus or other species.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Most organisms other than staphylococci are inhibited by the high salt concentration found in Mannitol Salt Agar except for some halophillic marine organisms.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">A few strains of&nbsp;<em>Staphylococcus aureus<\/em>&nbsp;may exhibit a delayed fermentation of mannitol. Negative plates should be re-incubated overnight before discarding.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Presumptive&nbsp;<em>Staphylococcus aureus<\/em>&nbsp;must be confirmed with a coagulase test.<\/span><\/li>\n<\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"references\"><\/span><span style=\"color: #000000;\"><strong>References <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol>\n<li><span style=\"color: #000000;\">HiMedia.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Mannitol Salt Agar (MSA) (Chapman Medium) European Pharmacopoeia, Usp.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Hardy Diagnostics.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">Mannitol Salt Agar. Dehydrated Culture Media. Thermo Fisher Scientific Inc.<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">https:\/\/en.wikipedia.org\/wiki\/Mannitol_salt_agar<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">http:\/\/microbeonline.com\/mannitol-salt-agar-msa-composition-uses-and-colony-characteristics\/<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">http:\/\/www.vumicro.com\/vumie\/help\/VUMICRO\/Mannitol_Salt_Agar.htm<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\">https:\/\/microbiologyinfo.com\/mannitol-salt-agar-for-the-isolation-of-staphylococcus-aureus\/<\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;\"><a href=\"https:\/\/www.sigmaaldrich.com\/content\/dam\/sigma-aldrich\/docs\/Sigma\/Datasheet\/4\/m9052dat.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.sigmaaldrich.com\/content\/dam\/sigma-aldrich\/docs\/Sigma\/Datasheet\/4\/m9052dat.pdf<\/a><\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of\u00a0Staphylococcus aureus\u00a0from clinical and non-clinical specimens. It is recommended for the detection and &#8230; <a title=\"Mannitol Salt Agar (MSA)- Composition, Principle, Preparation, Results, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/mannitol-salt-agar-msa\/\" aria-label=\"Read more about Mannitol Salt Agar (MSA)- Composition, Principle, Preparation, Results, Uses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":1143,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[236,2173,2176,2174,2180,2177,2175,2178,2179,237,243],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Results-on-Mannitol-Salt-Agar-MSA.jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/1142"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=1142"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/1142\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/1143"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=1142"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=1142"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=1142"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}