{"id":1072,"date":"2022-01-07T08:50:00","date_gmt":"2022-01-07T03:05:00","guid":{"rendered":"https:\/\/microbenotes.com\/?p=1072"},"modified":"2022-01-08T03:11:27","modified_gmt":"2022-01-07T21:26:27","slug":"xylose-lysine-deoxycholate-xld-agar","status":"publish","type":"post","link":"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/","title":{"rendered":"XLD Agar- Composition, Principle, Preparation, Results, Uses"},"content":{"rendered":"\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_65 counter-hierarchy ez-toc-counter ez-toc-custom ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title \" >Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #0a0a0a;color:#0a0a0a\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #0a0a0a;color:#0a0a0a\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/#what-is-xld-agar\" title=\"What is XLD Agar?\">What is XLD Agar?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/#composition-of-xld-agar\" title=\"Composition\u00a0of XLD Agar\">Composition\u00a0of XLD Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/#principle-of-xld-agar\" title=\"Principle of XLD Agar\">Principle of XLD Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/#preparation-of-xld-agar\" title=\"Preparation&nbsp;of XLD Agar\">Preparation&nbsp;of XLD Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/#results-of-xld-agar\" title=\"Results of XLD Agar\">Results of XLD Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/#uses-of-xld-agar\" title=\"Uses&nbsp;of XLD Agar\">Uses&nbsp;of XLD Agar<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/#limitations-of-xld-agar\" title=\"Limitations of XLD Agar \">Limitations of XLD Agar <\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"what-is-xld-agar\"><\/span><span style=\"color: #000000;\"><strong>What is XLD Agar<\/strong><\/span>?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\"><strong>Xylose\u00a0Lysine\u00a0Deoxycholate\u00a0(XLD) agar<\/strong> is a\u00a0selective\u00a0growth medium\u00a0used for the isolation of <em>Salmonella<\/em> and <em>Shigella<\/em> species from clinical samples and from food. XLD Agar was developed by Taylor for the differentiation, isolation, and identification of enteric pathogens, and to support the growth of more fastidious enteric organisms. XLD Agar was specially designed to allow the growth of\u00a0<em>Shigella<\/em>\u00a0species and is a proven medium for the isolation of this organism. It has also been found to be an excellent medium for isolating\u00a0<em>Salmonella\u00a0<\/em>species as well.\u00a0It has a\u00a0pH\u00a0of approximately 7.4, leaving it with a bright pink or red appearance due to the indicator phenol red. Sugar\u00a0fermentation\u00a0lowers the pH and the phenol red indicator registers this by changing to yellow. Most gut bacteria, including\u00a0<em>Salmonella<\/em>, can ferment the sugar\u00a0xylose\u00a0to produce acid;\u00a0<em>Shigella<\/em>\u00a0colonies cannot do this and therefore remain red. After exhausting the xylose supply\u00a0<em>Salmonella<\/em>\u00a0colonies will decarboxylate lysine, increasing the pH once again to alkaline and mimicking the red\u00a0<em>Shigella<\/em> colonies. Salmonellae metabolize thiosulfate to produce\u00a0hydrogen sulfide, which leads to the formation of colonies with black centers and allows them to be differentiated from the similarly colored\u00a0<em>Shigella<\/em>\u00a0colonies.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"composition-of-xld-agar\"><\/span><span style=\"color: #000000;\"><strong>Composition\u00a0of XLD Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Ingredients <\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Gms\/Litre<\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Yeast extract<\/span><\/td><td><span style=\"color: #000000;\">3.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">L- Lysine<\/span><\/td><td><span style=\"color: #000000;\">5.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Lactose<\/span><\/td><td><span style=\"color: #000000;\">7.5<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sucrose<\/span><\/td><td><span style=\"color: #000000;\">7.5<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Xylose<\/span><\/td><td><span style=\"color: #000000;\">3.5<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium chloride<\/span><\/td><td><span style=\"color: #000000;\">5.0<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium deoxycholate<\/span><\/td><td><span style=\"color: #000000;\">2.5<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Sodium thiosulphate<\/span><\/td><td><span style=\"color: #000000;\">6.8<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Ferric ammonium citrate<\/span><\/td><td><span style=\"color: #000000;\">0.8<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Phenol red<\/span><\/td><td><span style=\"color: #000000;\">0.08<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\">Agar<\/span><\/td><td><span style=\"color: #000000;\">15.0<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><span style=\"color: #000000;\">Final pH (at 25\u00b0C) 7.4\u00b10.2<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"principle-of-xld-agar\"><\/span><span style=\"color: #000000;\"><strong>Principle of XLD Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><span style=\"color: #000000;\">XLD Agar is both a selective and differential medium. The medium contains yeast extract, which provides nitrogen and vitamins required for growth. It utilizes sodium deoxycholate as the selective agent and, therefore, is inhibitory to gram-positive micro-organisms. Though the sugars xylose, lactose,<\/span> and sucrose provide sources of fermentable carbohydrates, xylose is mainly incorporated into the medium since it is not fermented by Shigella but practically by all enterics. This aids in the differentiation of Shigella species. Sodium chloride maintains the osmotic balance of the medium. Lysine is included to differentiate the Salmonella group from the non-pathogens. Salmonella rapidly ferments xylose and exhausts the supply. Subsequently, lysine is decarboxylated by the enzyme lysine decarboxylase to form amines with reversion to an alkaline pH that mimics the Shigella reaction. However, to prevent this reaction by lysine-positive coliforms, lactose and sucrose are added to produce acid in excess. Degradation of xylose, lactose, and sucrose to acid causes phenol red indicator to change its color to yellow. Bacteria that decarboxylate lysine to cadaverine can be recognized by the appearance of a red coloration around the colonies due to an increase in pH. These reactions can proceed simultaneously or successively, and this may cause the pH indicator to exhibit various shades of color or it may change its color from yellow to red on prolonged incubation. To add to the differentiating ability of the formulation, an H2S indicator system, consisting of sodium thiosulphate and ferric ammonium citrate, is included for the visualization of hydrogen sulfide<span style=\"color: #000000;\"> produced, resulting in the formation of colonies with black centers. The non-pathogenic H2S producers do not decarboxylase lysine; therefore, the acid reaction produced by them prevents the blackening of the colonies.<\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"preparation-of-xld-agar\"><\/span><span style=\"color: #000000;\"><strong>Preparation&nbsp;of XLD Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Suspend 56.68 grams in 1000 ml distilled water. <\/span><\/li><li><span style=\"color: #000000;\">Heat with frequent agitation until the medium boils.<br><em>DO NOT AUTOCLAVE OR OVERHEAT.<\/em><\/span><\/li><li><span style=\"color: #000000;\">Transfer immediately to a water bath at 50\u00b0C. <\/span><\/li><li><span style=\"color: #000000;\">After cooling, pour into sterile Petri plates. <\/span><\/li><li><span style=\"color: #000000;\">It is advisable not to prepare large volumes that will require prolonged heating, thereby producing precipitate, thereby producing precipitate.<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"results-of-xld-agar\"><\/span><span style=\"color: #000000;\"><strong>Results of XLD Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"420\" src=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Salmonella-Typhimurium-on-XLD-agar..jpg\" alt=\"Salmonella Typhimurium on XLD agar.\" class=\"wp-image-1073\" srcset=\"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Salmonella-Typhimurium-on-XLD-agar..jpg 800w, https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Salmonella-Typhimurium-on-XLD-agar.-300x158.jpg 300w, https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Salmonella-Typhimurium-on-XLD-agar.-768x403.jpg 768w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td><span style=\"color: #000000;\"><strong>Organisms <\/strong><\/span><\/td><td><span style=\"color: #000000;\"><strong>Colony characteristics <\/strong><\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Salmonella<\/em> H2S positive<\/span><\/td><td><span style=\"color: #000000;\">Red colonies with black centers<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Shigella<\/em> spp. and <em>Salmonella<\/em> H2S negative<\/span><\/td><td><span style=\"color: #000000;\">Red&nbsp; colonies<\/span><\/td><\/tr><tr><td><em><span style=\"color: #000000;\">E. coli<\/span><\/em><\/td><td><span style=\"color: #000000;\">Large, flat, yellow colonies<\/span><\/td><\/tr><tr><td><span style=\"color: #000000;\"><em>Proteus<\/em> spp.<\/span><\/td><td><span style=\"color: #000000;\">Red to Yellow colonies<\/span><\/td><\/tr><tr><td><em><span style=\"color: #000000;\">Enterobacter \/ Klebsiella<\/span><\/em><\/td><td><span style=\"color: #000000;\">Mucoid, yellow colonies<\/span><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"uses-of-xld-agar\"><\/span><span style=\"color: #000000;\"><strong>Uses&nbsp;of XLD Agar<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">XLD Agar is a selective differential medium for the isolation of Gram-negative enteric pathogens from fecal specimens and other clinical material.<\/span><\/li><li><span style=\"color: #000000;\">It is especially suitable for the isolation of&nbsp;<em>Shigella<\/em>&nbsp;and&nbsp;<em>Salmonella<\/em>&nbsp;species.<\/span><\/li><li><span style=\"color: #000000;\">Microbiological testing of foods, water and dairy products.<\/span><\/li><\/ol>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"limitations-of-xld-agar\"><\/span><span style=\"color: #000000;\"><strong>Limitations of XLD Agar <\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ol><li><span style=\"color: #000000;\">Some <em>Proteus<\/em> strains may give red to yellow colouration with most colonies developing black centers, giving rise to false positive reactions.<\/span><\/li><li><span style=\"color: #000000;\">Non-enterics like <em>Pseudomonas<\/em> and <em>Providencia<\/em> may exhibit red colonies.<\/span><\/li><li><span style=\"color: #000000;\">S. Paratyphi A, S. Choleraesuis, S. Pullorum and S. Gallinarum may form red colonies without H2S, thus resembling <em>Shigella<\/em><\/span>.<\/li><li><span style=\"color: #000000;\">Incubation in excess of 48 hours may lead to false-positive results.<\/span><\/li><li><span style=\"color: #000000;\">It is recommended that biochemical, immunological, molecular, or <a href=\"https:\/\/microbenotes.com\/mass-spectrometry-ms-principle-working-instrumentation-steps-applications\/\" target=\"_blank\" rel=\"noreferrer noopener\">mass spectrometry<\/a> testing be performed on colonies from pure culture for complete identification.<\/span><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"<p>What is XLD Agar? Xylose\u00a0Lysine\u00a0Deoxycholate\u00a0(XLD) agar is a\u00a0selective\u00a0growth medium\u00a0used for the isolation of Salmonella and Shigella species from clinical samples and from food. XLD Agar was developed by Taylor for &#8230; <a title=\"XLD Agar- Composition, Principle, Preparation, Results, Uses\" class=\"read-more\" href=\"https:\/\/microbenotes.com\/xylose-lysine-deoxycholate-xld-agar\/\" aria-label=\"Read more about XLD Agar- Composition, Principle, Preparation, Results, Uses\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":1073,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[703],"tags":[2021,2019,2018,2020,2023,2022,252,2010,2009,2015,2012,2011,2013,2014,2016,2017],"custom":{"td_video":"","featured_image":"https:\/\/microbenotes.com\/wp-content\/uploads\/2018\/08\/Salmonella-Typhimurium-on-XLD-agar..jpg","author":{"name":"Sagar Aryal","avatar":"https:\/\/secure.gravatar.com\/avatar\/862472ea06500d4318f1ad82addb608e?s=96&d=mm&r=g"},"categories":[{"term_id":703,"name":"Culture Media","slug":"culture-media","term_group":0,"term_taxonomy_id":703,"taxonomy":"category","description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","parent":0,"count":67,"filter":"raw","cat_ID":703,"category_count":67,"category_description":"<strong>Culture media or growth media is a liquid or gel support media provided with essential nutrients and growth parameters required for the growth of microorganisms.<\/strong>\r\n<ul>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are of different types depending on the nutrients they have and the type of microorganisms that grow on them.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Growth media are primarily of two types; one for cell culture where specific cell types are grown of specific plants and animals, and another for microbiological culture to support the growth of microorganisms on artificial surfaces.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microbial culture, the most common culture media are agar plates or broth medium. Agar plates are made up of a growth medium containing agar that results in the formation of a gel-like support medium for growth.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The broth medium, in turn, is made without any agar, which gives it the consistency of a liquid support medium.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Both the agar plates and broth media can be either general media or selective media.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">General media support the growth of multiple organisms, while selective media allow the selective growth of fastidious organisms that require specific nutrient and environmental requirements.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">For microorganisms like viruses or parasites, a growth medium containing living cells are required.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">All culture media have a list of common ingredients like a source of carbon, a source of protein or nitrogen, some salts, and water.\u00a0<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">In the case of culture media used for a specific purpose; however, additional ingredients like organic compounds or pH indicators might also be present.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The most common general culture media for the isolation of bacteria is the Nutrient Agar that supports the growth of a diverse group of bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Culture media are an important part of routine laboratory research as it allows the artificial culture of microorganisms under laboratory conditions and also helps in isolation and differentiation of different microorganisms.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">The growth pattern and colony morphology of certain bacteria on certain growth media provide a basis for the identification of the bacteria.<\/span><\/li>\r\n \t<li style=\"font-weight: 400\"><span style=\"font-weight: 400\">Traditionally, this technique was one of the most important methods of identification of microorganisms. It is still employed in many laboratories throughout the world for diagnostic and educational purposes.<\/span><\/li>\r\n<\/ul>","cat_name":"Culture Media","category_nicename":"culture-media","category_parent":0}]},"_links":{"self":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/1072"}],"collection":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/comments?post=1072"}],"version-history":[{"count":0,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/posts\/1072\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media\/1073"}],"wp:attachment":[{"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/media?parent=1072"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/categories?post=1072"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microbenotes.com\/wp-json\/wp\/v2\/tags?post=1072"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}