Vmax

Lineweaver–Burk Plot Since, Vmax is achieved at infinite substrate concentration, it is impossible to estimate Vmax and hence Km from a hyperbolic plot. Because of this difficulty, the Michaelis–Menten equation was transformed into an equation for a straight line by Lineweaver and Burk. The Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation … Read moreLineweaver–Burk Plot