Alkaline Peptone Water

Alkaline Peptone Water

Alkaline Peptone Water Alkaline Peptone Water is a broth medium for the enrichment of Vibrio species from food, water and clinical samples. Shread, et al. formulated peptone water for use as a nonselective enrichment broth for the cultivation of Enterobacteriaceae from foodstuffs and other specimens. Alkaline Peptone Water is a modification of peptone water with the … Read moreAlkaline Peptone Water

Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar

Result Interpretation on Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar

Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar Thiosulfate-citrate-bile salts-sucrose agar, or TCBS agar, is a type of selective agar that is used in microbiology laboratories to isolate Vibrio species. TCBS Agar is a selective differential medium for isolating and cultivating Vibrio cholerae and other Vibrio species from clinical specimens and other materials. TCBS Agar was developed by Kobayashi et al, who modified the selective medium … Read moreThiosulfate-citrate-bile salts-sucrose (TCBS) Agar

Biochemical Test of Vibrio cholerae

Biochemical Test of Vibrio cholerae

Biochemical Test of Vibrio cholerae Image Source: Belén García, dibujandoafrica.com ­Basic Characteristics Properties (Vibrio cholerae) Capsule Non-Capsulated Citrate +ve Flagella Flagellated Gas -ve Gelatin Hydrolysis +ve Gram Staining -ve H2S -ve Hemolysis Beta Hemolysis Indole +ve Motility Motile MR (Methyl Red) -ve Nitrate Reduction +ve Oxidase +ve Shape Rods Spore Non-Sporing String Test +ve Urease … Read moreBiochemical Test of Vibrio cholerae

Vibrio cholerae (Owaga) on TCBS Agar

Vibrio cholerae (Owaga) on TCBS Agar

– Vibrio cholerae (Owaga) on TCBS Agar. – Photo By: Q.F.B. Carlos Rafael Manzano Palafox from México. – TCBS Agar is used for the isolation of Vibrio cholerae and other enteropathologic Vibrio (in particular Vibrio parahaemolyticus) in fish, seafood and biological samples of animal origin. – Flat yellow colonies, 2-3 mm in diameter