Biochemical Test of Bacteria Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are … Read more Biochemical Test of Bacteria
Answer: Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios. TCBS agar is composed of yeast extract, peptone, sodium thiosulfate, sodium citrate, ox bile (oxgall), sucrose, sodium chloride, iron (III) citrate, bromothymol blue, thymol blue, agar, and distilled or deionized water. TCBS is not autoclaved because: This … Read more Why TCBS agar is not autoclaved?
Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar Thiosulfate-citrate-bile salts-sucrose agar, or TCBS agar, is a type of selective agar that is used in microbiology laboratories to isolate Vibrio species. TCBS Agar is a selective differential medium for isolating and cultivating Vibrio cholerae and other Vibrio species from clinical specimens and other materials. TCBS Agar was developed by Kobayashi et al, who modified the selective medium … Read more Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar
Biochemical Test of Vibrio cholerae Image Source: Belén García, dibujandoafrica.com Basic Characteristics Properties (Vibrio cholerae) Capsule Non-Capsulated Citrate +ve Flagella Flagellated Gas -ve Gelatin Hydrolysis +ve Gram Staining -ve H2S -ve Hemolysis Beta Hemolysis Indole +ve Motility Motile MR (Methyl Red) -ve Nitrate Reduction +ve Oxidase +ve Shape Rods Spore Non-Sporing String Test +ve Urease … Read more Biochemical Test of Vibrio cholerae
– Vibrio cholerae (Owaga) on TCBS Agar. – Photo By: Q.F.B. Carlos Rafael Manzano Palafox from México. – TCBS Agar is used for the isolation of Vibrio cholerae and other enteropathologic Vibrio (in particular Vibrio parahaemolyticus) in fish, seafood and biological samples of animal origin. – Flat yellow colonies, 2-3 mm in diameter