Answer: Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar is used for the selective isolation of Vibrio cholerae and other enteropathogenic vibrios. TCBS agar is composed of yeast extract, peptone, sodium thiosulfate, sodium citrate, ox bile (oxgall), sucrose, sodium chloride, iron (III) citrate, bromothymol blue, thymol blue, agar, and distilled or deionized water. TCBS is not autoclaved because: This … Read more Why TCBS agar is not autoclaved?
Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar Thiosulfate-citrate-bile salts-sucrose agar, or TCBS agar, is a type of selective agar that is used in microbiology laboratories to isolate Vibrio species. TCBS Agar is a selective differential medium for isolating and cultivating Vibrio cholerae and other Vibrio species from clinical specimens and other materials. TCBS Agar was developed by Kobayashi et al, who modified the selective medium … Read more Thiosulfate-citrate-bile salts-sucrose (TCBS) Agar
– Vibrio cholerae (Owaga) on TCBS Agar. – Photo By: Q.F.B. Carlos Rafael Manzano Palafox from México. – TCBS Agar is used for the isolation of Vibrio cholerae and other enteropathologic Vibrio (in particular Vibrio parahaemolyticus) in fish, seafood and biological samples of animal origin. – Flat yellow colonies, 2-3 mm in diameter