The Gram stain was developed by Christian Gram in 1884 and modified by Hucker in 1921. The objective of Gram Stain This test differentiates the bacteria into Gram-Positive and Gram-Negative Bacteria, which helps in the classification and differentiation of microorganisms. … Read more
Hematoxylin and eosin staining technique functions to recognize different types of tissues and their morphological changes, especially in cancer diagnosis. Hematoxylin has a deep blue-purple color and stains nucleic acids by a complex, incompletely understood reaction. Eosin is pink and … Read more
Special stains have been developed over time for identifying bacteria species, differentiating them morphologically, and even characterizing there very special features. The most common stain being Gram Staining, Acid-fast staining, endospore staining. Each of these stains aims at identifying and … Read more
Introduction A normal bacterial cell is known as a vegetative form of a bacterial cell. It can multiply freely when provided with conducive environmental conditions like the provision of nutrients, temperature, water, and oxygen. But some groups of bacteria, when … Read more
Sudan Black B Staining definition Sudan stain is a special stain used for staining of fats and fat droplets using several Sudan dyes, which include Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B. These Sudan … Read more
Masson’s Trichrome Staining definition Masson’s Trichrome Staining is a histological staining method used for selectively stain collagen, collagen fibers, fibrin, muscles, and erythrocytes. It uses three stains for staining hence the term Trichrome. These are Weigert’s Hematoxylin, Biebrich scarlet-acid fuschin … Read more
Wheatley Trichrome Staining definition Wheatley Trichrome staining technique is a special permanent satin used in parasitology for the detection and identification of intestine protozoans from stool samples. Trichrome staining is performed on a PVA-fixed or Schaudinn’s- Preserved stool sample. The … Read more
What is positive staining? Positive staining of viruses is similar to negative staining. The difference is that the virus image is darker formed on a light background, unlike the negative staining where a light viral particle image is formed on … Read more
What is Periodic Acid-Schiff (PAS) Staining? Periodic-Acid Schiff (PAS) staining technique is used in histochemistry and histological studies to demonstrate the presence of carbohydrates and carbohydrate compounds such as polysaccharides, mucin, glycogen, and fungal cell wall components. It has been … Read more
What is Collagen Hybridizing Peptide Staining? Collagen is the major building block of all tissues including tendon, ligament, cornea, cartilage, and bone It is a fibrous, structural protein that made up of a right-handed bundle of three parallel, left-handed polyproline II-type … Read more
What is Iron-Hematoxylin Staining? This is a traditional staining technique commonly used for the detection of intestinal protozoans majorly because of its permanence and ability to demonstrate, observe, and quantify with high clarity protozoans nuclear structures. Iron hematoxylin was the stain used … Read more
What is Auramine- Rhodamine Staining? The evolution in staining methodologies has led to an era of modified staining techniques that are rapid, more versatile, and reliable in result interpretation. This is one of those techniques which was modified from the … Read more