Spectroscopy Archives | Microbe Notes

22 Types of Spectroscopy with Definition, Principle, Steps, Uses

November 12, 2020 by Anupama Sapkota

What is spectroscopy? Spectroscopy is the study of the interaction between light and matter where the absorption and emission of light or other radiation by the matter are studied and measured. Spectroscopy mainly deals with the dispersion of light and other radiations that is caused by an object which allows the study of various properties of the object. The measurement …

Gamma-ray (γ-ray) Spectroscopy

February 4, 2021February 20, 2019 by Sagar Aryal

Gamma rays are an ultrahigh frequency of light that is emitted by radioactive elements, energetic celestial bodies such as black holes and neutron stars, and high energy events such as nuclear explosions and supernovae. Gamma-ray (γ-ray) spectroscopy is a quick and nondestructive analytical technique that can be used to identify various radioactive isotopes in a sample. A gamma-ray spectrometer (GRS) is an …

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Infrared (IR) Spectroscopy

February 4, 2021December 8, 2018 by Sagar Aryal

Infrared (IR) spectroscopy or vibrational spectroscopy is an analytical technique that takes advantage of the vibrational transitions of a molecule. It is one of the most common and widely used spectroscopic techniques employed mainly by inorganic and organic chemists due to its usefulness in determining structures of compounds and identifying them. The method or technique of infrared spectroscopy is conducted …

Nuclear Magnetic Resonance (NMR) Spectroscopy

February 4, 2021November 12, 2018 by Sagar Aryal

Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique to observe local magnetic fields around atomic nuclei. It is a spectroscopy technique which is based on the absorption of electromagnetic radiation in the radio frequency region 4 to 900 MHz by nuclei of the atoms. Over the past fifty years, NMR has become the preeminent technique …

UV Spectroscopy- Principle, Instrumentation, Applications

February 4, 2021November 11, 2018 by Sagar Aryal

Spectroscopy is the measurement and interpretation of electromagnetic radiation absorbed or emitted when the molecules or atoms or ions of a sample moves from one energy state to another energy state. UV spectroscopy is type of absorption spectroscopy in which light of ultra-violet region (200-400 nm) is absorbed by the molecule which results in the excitation of the electrons from …