Sandwich ELISA- Steps and Advantages

Sandwich ELISA- Steps and Advantages

The sandwich ELISA quantify antigens between two layers of antibodies (i.e. capture and detection antibody). Sandwich ELISA is named so as antigen is sandwiched between two antibodies. The sandwich assay uses two different antibodies that are reactive with different epitopes … Read more

Enzyme-linked immunosorbent assay (ELISA)

Enzyme-linked immunosorbent assay (ELISA) and its types

Enzyme-linked immunosorbent assay (ELISA) Enzyme-linked immunosorbent assay (ELISA) utilizes an enzyme system to show specific combination of an antigen with its antibody. It is a method of quantifying an antigen immobilized on a solid surface. ELISA uses a specific antibody … Read more