Differences between DNA and RNA (DNA vs RNA)

Differences between DNA and RNA

Differences between DNA and RNA (DNA vs RNA) Some of the differences are: S.N. Character DNA RNA 1.       Full form Deoxyribonucleic Acid  Ribonucleic Acid 2.       Location DNA is found in the nucleus, with a small amount of DNA also present in mitochondria. RNA forms in the nucleolus, and then moves to specialized regions … Read more Differences between DNA and RNA (DNA vs RNA)

RNA Isolation Protocol

RNA Isolation Protocol

RNA Isolation Protocol Total RNA isolation from Bacterial Cells Principle of RNA Isolation Total RNA is isolated and separated from DNA and protein after extraction with a solution called as Trizol. Trizol is an acidic solution containing guanidinium thiocyanate (GITC), phenol and chloroform. GITC irreversibly denatures proteins and RNases. This is followed by centrifugation. Under … Read more RNA Isolation Protocol

RNA- Properties, Structure, Types and Functions

RNA- Properties, Structure, Types and Functions

RNA- Properties, Structure, Types and Functions RNA or ribonucleic acid is a polymer of nucleotides which is made up of a ribose sugar, a phosphate, and bases such as adenine, guanine, cytosine, and uracil. It is a polymeric molecule essential in various biological roles in coding, decoding, regulation, and expression of genes. Figure: (a) Ribonucleotides contain the pentose sugar ribose instead of the deoxyribose … Read more RNA- Properties, Structure, Types and Functions

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation

Acridine Orange Stain- Principle, Procedure and Result Interpretation Objective Differential staining of nucleic acids including DNA and RNA. Principle Acridine orange, a vital stain, will intercalate with nucleic acid, changing the dye’s optical characteristics so that it will fluoresce bright orange under ultraviolet light. All nucleic acid–containing cells will fluoresce orange. Acridine orange is a … Read more Acridine Orange Stain- Principle, Procedure and Result Interpretation