Cetrimide Agar Test- Principle, Procedures, Results, Uses

Cetrimide Agar Test- Principle, Procedure and Result Interpretation

What is Cetrimide Agar Test? Cetrimide agar test is a biochemical test performed to identify or differentiate Pseudomonas aeruginosa from other microorganisms. The test works on the principle of the ability of an organism to grow in the presence of cetrimide. Cetrimide is a toxic quaternary ammonium detergent that is toxic to most bacteria except for few organisms like P. …

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Acetamide Utilization Test- Principle, Procedure, Results, Uses

Acetamide Utilization Test- Principle, Procedure and Expected Results

What is Acetamide Utilization Test? The Acetamide utilization test is one of the many biochemical tests performed for the identification of aerobic organisms. This test is based on the utilization of acetamide by the organism via the process of deamidation. The deamidation process occurs in the presence of acylamidase enzyme. An Acetamide utilization test is performed for the distinction between …

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Biochemical Test of Bacteria

Biochemical Test of Bacteria

Biochemical Test of Bacteria Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are necessary for the bacterium’s individual …

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Cetrimide Agar

Result Interpretation of Cetrimide Agar

Cetrimide Agar Introduction of Cetrimide Agar Cetrimide is a quaternary ammonium salt, which acts as a cationic detergent that reduces surface tension in the point of contact and has precipitant, complexing and denaturing effects on bacterial membrane proteins. It exhibits inhibitory actions on a wide variety of microorganisms including Pseudomonas species other than Pseudomonas aeruginosa. Cetrimide agar was first developed …

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Biochemical Test of Pseudomonas aeruginosa

Biochemical Test of Pseudomonas aeruginosa

Biochemical Test of Pseudomonas aeruginosa Basic Characteristics Properties (Pseudomonas aeruginosa) Capsule Non-Capsulated Catalase Positive (+ve) Cetrimide Test Positive (+ve) Citrate Positive (+ve) Coagulase Negative (-ve) Flagella Flagellated (Single) Gas Negative (-ve) Gelatin Hydrolysis Positive (+ve) Gram Staining Negative (-ve) H2S Negative (-ve) Hemolysis Beta Hemolytic Indole Negative (-ve) Motility Motile (Unipolar) MR (Methyl Red) Negative (-ve) Nitrate Reduction Positive (+ve) …

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