New York City Agar

New York City Agar

Introduction NYC Agar Base medium was originally developed by Fauer, Weisburd and Wilson at the New York City Department of Health for selective isolation of pathogenic Neisseria species from clinical specimens. NYC medium, primarily designed for isolation of pathogenic Neisseria, also readily supports the growth of large-colony mycoplasmas and T-mycoplasmas (Ureaplasma). It is a transparent medium, … Read more New York City Agar

Martin Lewis Agar

Martin Lewis Agar

Introduction Thayer-Martin Agar was developed in 1964 for the isolation of N. gonorrhoeae and N. meningitidis. A further modification of these early formulations was developed to improve the isolation of pathogenic Neisseria sp. from specimens, which contained large amounts of mixed microbial flora. Martin-Lewis Agar is the formulation made after modification which contains an increased … Read more Martin Lewis Agar

Neisseria gonorrhoeae- Laboratory Diagnosis, Treatment, Prevention

Neisseria gonorrhoeae- Laboratory Diagnosis, Treatment, Prevention

Neisseria gonorrhoeae is an obligate human pathogen and is the etiological agent of gonorrhea. In the United States, it is the second most commonly reported communicable disease, with more than 350,000 cases reported annually. Syndromes include cervicitis in women and urethritis, pharyngitis and proctitis in both sexes. If untreated, women may experience severe sequelae of … Read more Neisseria gonorrhoeae- Laboratory Diagnosis, Treatment, Prevention

Modified Thayer Martin Agar

Modified Thayer Martin Agar

Modified Thayer Martin Agar Thayer and Martin reported an improvement of the Chocolate Agar formulation that contained antimicrobics vancomycin, colistin and nystatin have been added to minimize overgrowth by contaminants, suppress the growth of saprophytic Neisseria sp., and enhance the growth of pathogenic Neisseria. Martin and Lester later added antibiotic trimethoprim to make the medium … Read more Modified Thayer Martin Agar

Cystine Tryptic Agar

Cystine Tryptic Agar

Cystine Tryptic Agar Cystine tryptic agar (CTA), also known as cystine trypticase agar, was developed by Vera as a semi-solid growth medium used for the identification and maintenance of microorganisms for extended periods when stored at appropriate temperatures. It is used for the detection of bacterial motility and, with added carbohydrate, it contributes to fermentation … Read more Cystine Tryptic Agar

Chocolate Agar

Chocolate Agar

Chocolate Agar Chocolate agar is essentially the same as blood agar except that during preparation the red blood cells are lysed when added to molten agar base. As a result, the cell lysis releases intracellular nutrients such as hemoglobin, hemin (“X” factor), and the coenzyme nicotinamide adenine dinucleotide (NAD or “V” factor) into the agar … Read more Chocolate Agar

Differences between Neisseria meningitidis and Neisseria gonorrhoeae

Differences between Neisseria meningitidis and Neisseria gonorrhoeae

Differences between Neisseria meningitidis and Neisseria gonorrhoeae Neisseria meningitidis and Neisseria gonorrhoeae, are obligate human pathogens that cause distinctly different disease syndromes. They are both Gram negative diplococci, non-sporing, oxidase positive and difficult to differentiate on morphological and cultural characteristics. However, they can be differentiated on the following grounds: S.N. Character Neisseria gonorrhoeae Neisseria meningitidis 1.       Commonly … Read more Differences between Neisseria meningitidis and Neisseria gonorrhoeae

Biochemical Test of Neisseria gonorrhoeae

Biochemical Test of Neisseria gonorrhoeae

Biochemical Test of Neisseria gonorrhoeae ­Basic Characteristics Properties ( Neisseria gonorrhoeae) Capsule Negative (-ve) Catalase Positive (+ve) Gas Negative (-ve) Gram Staining Negative (-ve) H2S Negative (-ve) Hemolysis Negative (-ve) Nitrate Reduction Negative (-ve) Oxidase Positive (+ve) Pigment Negative (-ve) Shape Diplococci Fermentation of DNase Negative (-ve) Fructose Negative (-ve) Glucose Positive (+ve) Lactose Negative … Read more Biochemical Test of Neisseria gonorrhoeae

Pathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Pathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Virulence factors or antigenic structures of Neisseria gonorrhoeae A. Pili Pili are the hair like appendages that extend up to several micrometers from the gonococcal surface. It mediates the exchange of genetic material between strains and attachment to human mucosal cell surface, invasion of host cells, and survival through the inhibition of phagocytosis. Two types: … Read more Pathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Habitat and Morphology of Neisseria gonorrhoeae

Habitat and Morphology of Neisseria gonorrhoeae

Habitat and Morphology of Neisseria gonorrhoeae Habitat of Neisseria gonorrhoeae Human is only the natural host. Found in human urogenital tract. Rectal carriage in healthy individuals. Typically seen in pus cells (intracellular) Found intracellularly in polymorphonuclear leukocytes, or a specific category of white blood cells with varying shapes of nuclei. Morphology of Neisseria gonorrhoeae Gram –ve … Read more Habitat and Morphology of Neisseria gonorrhoeae