Neisseria gonorrhoeae- Laboratory Diagnosis, Treatment, Prevention

Neisseria gonorrhoeae- Laboratory Diagnosis, Treatment, Prevention

Neisseria gonorrhoeae is an obligate human pathogen and is the etiological agent of gonorrhea. In the United States, it is the second most commonly reported communicable disease, with more than 350,000 cases reported annually. Syndromes include cervicitis in women and urethritis, pharyngitis and proctitis in both sexes. If untreated, women may experience severe sequelae of … Read moreNeisseria gonorrhoeae- Laboratory Diagnosis, Treatment, Prevention

Modified Thayer Martin Agar

Modified Thayer Martin Agar

Modified Thayer Martin Agar Thayer and Martin reported an improvement of the Chocolate Agar formulation that contained antimicrobics vancomycin, colistin and nystatin have been added to minimize overgrowth by contaminants, suppress the growth of saprophytic Neisseria sp., and enhance the growth of pathogenic Neisseria. Martin and Lester later added antibiotic trimethoprim to make the medium … Read moreModified Thayer Martin Agar

Differences between Neisseria meningitidis and Neisseria gonorrhoeae

Differences between Neisseria meningitidis and Neisseria gonorrhoeae

Differences between Neisseria meningitidis and Neisseria gonorrhoeae Neisseria meningitidis and Neisseria gonorrhoeae, are obligate human pathogens that cause distinctly different disease syndromes. They are both Gram negative diplococci, non-sporing, oxidase positive and difficult to differentiate on morphological and cultural characteristics. However, they can be differentiated on the following grounds: S.N. Character Neisseria gonorrhoeae Neisseria meningitidis 1.       Commonly … Read moreDifferences between Neisseria meningitidis and Neisseria gonorrhoeae

Biochemical Test of Neisseria gonorrhoeae

Biochemical Test of Neisseria gonorrhoeae

Biochemical Test of Neisseria gonorrhoeae ­Basic Characteristics Properties ( Neisseria gonorrhoeae) Capsule Negative (-ve) Catalase Positive (+ve) Gas Negative (-ve) Gram Staining Negative (-ve) H2S Negative (-ve) Hemolysis Negative (-ve) Nitrate Reduction Negative (-ve) Oxidase Positive (+ve) Pigment Negative (-ve) Shape Diplococci Fermentation of DNase Negative (-ve) Fructose Negative (-ve) Glucose Positive (+ve) Lactose Negative … Read moreBiochemical Test of Neisseria gonorrhoeae

Pathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Pathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Virulence factors or antigenic structures of Neisseria gonorrhoeae A. Pili Pili are the hair like appendages that extend up to several micrometers from the gonococcal surface. It mediates the exchange of genetic material between strains and attachment to human mucosal cell surface, invasion of host cells, and survival through the inhibition of phagocytosis. Two types: … Read morePathogenesis and Clinical Manifestation of Neisseria gonorrhoeae

Habitat and Morphology of Neisseria gonorrhoeae

Habitat and Morphology of Neisseria gonorrhoeae

Habitat and Morphology of Neisseria gonorrhoeae Habitat of Neisseria gonorrhoeae Human is only the natural host. Found in human urogenital tract. Rectal carriage in healthy individuals. Typically seen in pus cells (intracellular) Found intracellularly in polymorphonuclear leukocytes, or a specific category of white blood cells with varying shapes of nuclei. Morphology of Neisseria gonorrhoeae Gram –ve … Read moreHabitat and Morphology of Neisseria gonorrhoeae

Butyrate Disk Test- Principle, Procedure and Result Interpretation

Butyrate Disk Test- Principle, Procedure and Result Interpretation

Butyrate Disk Test- Principle, Procedure and Result Interpretation Purpose of Butyrate Disk Test This is a rapid test to detect the enzyme butyrate esterase, to aid identification of Moraxella (Branhamella) catarrhalis. Principle of Butyrate Disk Test Organisms capable of producing butyrate esterase hydrolyze bromochlorindolyl butyrate. Hydrolysis of the substrate in the presence of butyrate esterase releases indoxyl, … Read moreButyrate Disk Test- Principle, Procedure and Result Interpretation