Lowenstein Jensen (LJ) Media

Result Interpretation of Lowenstein Jensen (LJ) Media

Lowenstein Jensen (LJ) Media Lowenstein Jensen Medium (LJ Medium) is a selective medium that is commonly used for the cultivation and isolation of Mycobacterium, specifically Mycobacterium tuberculosis from clinical specimens. LJ medium was originally formulated by Lowenstein who incorporated congo red and malachite green to inhibit unwanted bacteria. The present formulation comprises of a glycerated egg-based medium and … Read moreLowenstein Jensen (LJ) Media

Biochemical Test of Mycobacterium tuberculosis

Biochemical Test of Mycobacterium tuberculosis Basic Characteristics Properties (Mycobacterium tuberculosis) 5 % NaCl Tolerance Negative (-ve) 68°C Catalase Test Negative (-ve) Acid Fast Stain Positive (-ve) Acid Phosphatase Negative (-ve) Amidase Test Positive (-ve) Arylsulphatase Test Negative (-ve) Growth at 22°C Negative (-ve) Growth at 25°C Negative (-ve) Growth at 42°C Negative (-ve) Growth at … Read moreBiochemical Test of Mycobacterium tuberculosis

Pathogenesis and Clinical Manifestations of Mycobacterium leprae

Pathogenesis and Clinical Manifestations of Mycobacterium leprae

Pathogenesis of Mycobacterium leprae M. leprae is an acid-fast, gram-positive obligate intracellular bacillus that shows tropism for cells of the reticuloendothelial system and peripheral nervous system (notably Schwann cells). Organisms may be acquired by the susceptible host usually through respiratory system or by way of skin to skin contact (between exudates of a leprosy patient’s skin … Read morePathogenesis and Clinical Manifestations of Mycobacterium leprae

Laboratory diagnosis of Leprosy caused by Mycobacterium leprae

Laboratory diagnosis of Leprosy caused by Mycobacterium leprae

Laboratory diagnosis of Leprosy caused by Mycobacterium leprae Specimen Skin biopsies, nasal discharges, scrapings from the nasal mucosa and slit-skin smears which are prepared by making superficial incisions in the skin, scraping out some tissue fluid and cells. Skin smears The skin smears are collected from the leprous lesions, such as nodules, thick papules, and … Read moreLaboratory diagnosis of Leprosy caused by Mycobacterium leprae

Laboratory diagnosis, treatment and prevention of Tuberculosis

Laboratory diagnosis, treatment and prevention of Tuberculosis

Laboratory diagnosis of Tuberculosis caused by Mycobacterium tuberculosis Specimen and processing sputum, bronchial washings, brushings or biopsies or early morning gastric aspirates, Cerebospinal Fluid (CSF), urine Specimens from sputum and other nonsterile sites should be liquefied with N-acetyl-L-cysteine decontaminated with NaOH (kills many other bacteria and fungi), neutralized with buffer, and concentrated by centrifugation. Specimens … Read moreLaboratory diagnosis, treatment and prevention of Tuberculosis

Pathogenesis and Clinical manifestation of Mycobacterium tuberculosis

Pathogenesis and Clinical manifestation of Mycobacterium tuberculosis

Virulence factors of Mycobacterium tuberculosis A. Mycolic acid and Lipoarabinomannan (LAM) Waxy layer that protects the bacteria from many host factors and also to many antibiotics including beta lactamases. Muramyl dipeptide (from peptidoglycan) complexed with mycolic acids can cause granuloma formation, phospholipids induce caseous necrosis. LAM is structurally and functionally related to the O antigenic … Read morePathogenesis and Clinical manifestation of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis Habitat Human is only the know reservoir. Usually found in the lungs but can also live in any part of the body. They may be obligate pathogen, facultative or opportunistic pathogens or free living. Usually infect the mono-nuclear phagocytes. Can also be infected by the consumption of unpasteurized milk. … Read moreHabitat and Morphology of Mycobacterium tuberculosis

Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation Objective of Kinyoun-Cold Method Identification of acid-fast Mycobacterium spp. and parasites such as Cryptosporidium and Isopora spp. Principle of Kinyoun-Cold Method Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining with aqueous based stains such as the Gram stain. … Read moreAcid Fast Stain (Kinyoun-Cold Method)- Principle, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Acid Fast Stain- Principle, Reagents, Procedure and Result Interpretation It is also know as Ziehl-Neelsen stain. Objective of Acid Fast Stain The main aim objective of this stain is to differentiate bacteria into acid fast group and non-acid fast groups. Principle of Acid Fast Stain Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells … Read moreAcid Fast Stain- Principle, Reagents, Procedure and Result Interpretation

Biochemical Characteristics of Mycobacterium tuberculosis

Biochemical Characteristics of Mycobacterium tuberculosis

Biochemical Characteristics of Mycobacterium tuberculosis S.N. Biochemical Test Results 1 5% NaCl Tolerance Negative (-ve) 2 68°C Catalase Test Negative (-ve) 3 Acid Phosphatase Negative (-ve) 4 Amidase Test Positive (+ve) 5 Arylsulphatase Test Negative (-ve) 6 Growth on P-Nitrobenzoic Acid Negative (-ve) 7 Growth on TCH (10mg/ml) Positive (+ve) 8 Iron Uptake Negative (-ve) … Read moreBiochemical Characteristics of Mycobacterium tuberculosis