Mycobacterium tuberculosis- An Overview

Mycobacterium tuberculosis

Habitat of Mycobacterium tuberculosis Human is only a known reservoir. Usually found in the lungs but can also live in any part of the body. They may be obligate pathogen, facultative or opportunistic pathogens, or free living. Usually infect the … Read more

Cultural Characteristics of Mycobacterium tuberculosis

Cultural Characteristics of Mycobacterium tuberculosis

Culture is the gold standard for laboratory confirmation of Tuberculosis. Growing bacteria are required to perform drug-susceptibility testing and genotyping. In the Lowenstein-Jensen (LJ) medium It is an egg-based medium and growth is quite slow. It takes 6-8 weeks to … Read more

Middlebrook Agar

Middlebrook Agar

There are many culture media that have been devised for the cultivation of mycobacteria over the years. The early ones were egg-based formulations which included Lowenstein-Jensen Medium and Petragnani Medium. Later, Dubos and Middlebrook developed various formulations containing oleic acid … Read more

Lowenstein Jensen (LJ) Media

Result Interpretation of Lowenstein Jensen (LJ) Media

Lowenstein Jensen Medium (LJ Medium) is a selective medium that is commonly used for the cultivation and isolation of Mycobacterium, specifically Mycobacterium tuberculosis from clinical specimens. LJ medium was originally formulated by Lowenstein who incorporated congo red and malachite green to inhibit … Read more

Biochemical Test of Mycobacterium tuberculosis

Biochemical Test of Mycobacterium tuberculosis Basic Characteristics Properties (Mycobacterium tuberculosis) 5 % NaCl Tolerance Negative (-ve) 68°C Catalase Test Negative (-ve) Acid Fast Stain Positive (-ve) Acid Phosphatase Negative (-ve) Amidase Test Positive (-ve) Arylsulphatase Test Negative (-ve) Growth at … Read more

Pathogenesis and Clinical Manifestations of Mycobacterium leprae

Pathogenesis and Clinical Manifestations of Mycobacterium leprae

Pathogenesis of Mycobacterium leprae M. leprae is an acid-fast, gram-positive obligate intracellular bacillus that shows tropism for cells of the reticuloendothelial system and peripheral nervous system (notably Schwann cells). Organisms may be acquired by the susceptible host usually through respiratory system … Read more

Laboratory diagnosis, treatment and prevention of Tuberculosis

Laboratory diagnosis, treatment and prevention of Tuberculosis

Laboratory diagnosis of Tuberculosis caused by Mycobacterium tuberculosis Specimen and processing sputum, bronchial washings, brushings or biopsies or early morning gastric aspirates, Cerebospinal Fluid (CSF), urine Specimens from sputum and other nonsterile sites should be liquefied with N-acetyl-L-cysteine decontaminated with … Read more

Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis

Habitat and Morphology of Mycobacterium tuberculosis Habitat Human is only the know reservoir. Usually found in the lungs but can also live in any part of the body. They may be obligate pathogen, facultative or opportunistic pathogens or free living. … Read more