Mycobacterium tuberculosis- An Overview
Habitat of Mycobacterium tuberculosis Human is only a known reservoir. Usually found in the lungs but can also live in any part of the body. They may be obligate pathogen, … Read more
Habitat of Mycobacterium tuberculosis Human is only a known reservoir. Usually found in the lungs but can also live in any part of the body. They may be obligate pathogen, … Read more
Biochemical Test of Mycobacterium tuberculosis Basic Characteristics Properties (Mycobacterium tuberculosis) 5 % NaCl Tolerance Negative (-ve) 68°C Catalase Test Negative (-ve) Acid Fast Stain Positive (+ve) Acid Phosphatase Negative (-ve) … Read more
Pathogenesis of Mycobacterium leprae M. leprae is an acid-fast, gram-positive obligate intracellular bacillus that shows tropism for cells of the reticuloendothelial system and peripheral nervous system (notably Schwann cells). Organisms may … Read more
Lowenstein Jensen (LJ) Media is a selective medium that is commonly used for the cultivation and isolation of Mycobacterium, specifically Mycobacterium tuberculosis from clinical specimens. LJ medium was originally formulated by … Read more
There are many culture media that have been devised for the cultivation of mycobacteria over the years. The early ones were egg-based formulations which included Lowenstein-Jensen Medium and Petragnani Medium. … Read more
Objective of Kinyoun-Cold Method Identification of acid-fast Mycobacterium spp. and parasites such as Cryptosporidium and Isopora spp. Principle of Kinyoun-Cold Method Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the … Read more
It is also know as Ziehl-Neelsen stain. Objective of Acid Fast Stain The main aim objective of this stain is to differentiate bacteria into acid fast group and non-acid fast groups. … Read more
Laboratory diagnosis of Leprosy caused by Mycobacterium leprae Specimen Skin biopsies, nasal discharges, scrapings from the nasal mucosa and slit-skin smears which are prepared by making superficial incisions in the … Read more