Middlebrook Agar
Middlebrook Agar There are many culture media that have been devised for the cultivation of mycobacteria over the years. The early ones were egg-based formulations which included Lowenstein-Jensen Medium and Petragnani Medium. Later, Dubos and Middlebrook developed various formulations containing oleic acid and albumin as key ingredients, which protect Mycobacterium from toxic agents, helping for the growth of tubercle bacilli. …