Lipase

Egg Yolk Agar- Composition, Principle, Preparation, Results, Uses

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Egg Yolk Agar

Egg Yolk Agar, modified is based on the original formula for Egg Yolk Agar developed by McClung and Toabe for the isolation and differentiation of organisms based on Lecithinase and lipase production and proteolytic activity. Image Source: Noura El-Ahmady El-Naggar, DOI: 10.5897/AJMR11.346 Composition of Egg Yolk Agar Ingredients Gms/liter Casein enzymic hydrolysate 15.000 Papaic digest of soybean meal 5.000 Yeast extract 5.000 Sodium chloride 5.000 L-Cystine 0.400 Hemin 0.005 Vitamin K1 0.010 Agar 20.000 Final pH (at 25°C): 7.5±0.2 Principle of … Read more

Lipase Test- Objectives, Principle, Procedures, Results, Uses

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Result Interpretation of Lipase Test

Objectives of Lipase Test The objective is to identify bacteria capable of producing the exoenzyme lipase. The Lipase Test is used to detect and enumerate lipolytic bacteria, especially in high-fat dairy products. A variety of other lipid substrates, including corn oil, olive oil, and soybean oil, are used to detect differential characteristics among members of Enterobacteriaceae, Clostridium, Staphylococcus, and Neisseria. Several fungal species also demonstrate lipolytic ability. Principle of Lipase Test Fats are formed by ester linkage between three molecules of fatty acids … Read more

Lipid Hydrolysis Test- Objectives, Principle, Procedure and Results

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Result Interpretation of Lipid Hydrolysis Test

Objectives of Lipid Hydrolysis Test To determine the ability of the organism to hydrolyse lipid. To identify bacteria capable of producing the exoenzyme lipase. Principle of Lipid Hydrolysis Test Lipids generally are nonpolar molecules that do not dissolve well in water.  Fats are one type of lipids that are large polymers of fatty acids and glycerol that are too large to enter the cell membrane.  In order to utilize fats, bacterial cells secrete exoenzymes known as lipases outside of the cell that hydrolyze the lipid … Read more