Lipase Archives | Microbe Notes

Lipase Test- Objectives, Principle, Procedures, Results, Uses

October 23, 2020October 22, 2020 by Sagar Aryal

Objectives of Lipase Test The objective is to identify bacteria capable of producing the exoenzyme lipase. The Lipase Test is used to detect and enumerate lipolytic bacteria, especially in high-fat dairy products. A variety of other lipid substrates, including corn oil, olive oil, and soybean oil, are used to detect differential characteristics among members of Enterobacteriaceae, Clostridium, Staphylococcus, and Neisseria. Several …

Egg Yolk Agar

January 6, 2020March 30, 2019 by Sagar Aryal

Egg Yolk Agar Egg Yolk Agar, modified is based on the original formula for Egg Yolk Agar developed by McClung and Toabe for the isolation and differentiation of organisms based on Lecithinase and lipase production and proteolytic activity. Image Source: Noura El-Ahmady El-Naggar, DOI: 10.5897/AJMR11.346 Composition of Egg Yolk Agar Ingredients Gms/litre Casein enzymic hydrolysate 15.000 Papaic digest of soyabean meal …

Lipid Hydrolysis Test- Objectives, Principle, Procedure and Results

January 13, 2020June 5, 2018 by Sagar Aryal

Result Interpretation of Lipid Hydrolysis Test

Objectives of Lipid Hydrolysis Test To determine the ability of the organism to hydrolyse lipid. To identify bacteria capable of producing the exoenzyme lipase. Principle of Lipid Hydrolysis Test Lipids generally are nonpolar molecules that do not dissolve well in water. Fats are one type of lipids that are large polymers of fatty acids and glycerol that are too large to enter the …