Buffered Charcoal Yeast Extract (BCYE)

Buffered Charcoal Yeast Extract (BCYE)

Feeley et al in 1978 developed a medium to isolate Legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract, and replacing starch with activated charcoal and named it as Charcoal Yeast Extract (CYE) Agar. … Read more

Laboratory diagnosis of Legionella pneumophila

Laboratory diagnosis of Legionella pneumophila

Laboratory diagnosis of Legionella pneumophila Specimen: Respiratory secretions (sputum, bronchial aspirate or washings), as well as pleural fluid, lung biopsy or autopsy material. Microscopy Legionellae in clinical specimens stain poorly with Gram stain and the small, intracellular bacteria are rarely … Read more