Online Microbiology and Biology Study Notes
Biochemical Test of Klebsiella granulomatis Image: Granuloma inguinale (donovanosis) in a conventional Pap test. A: Granulomatous inflammation is shown with epithelioid histiocytes and lymphocytes. B: The organisms (Donovan bodies) can be seen in thin-walled intracytoplasmic vacuoles (circle ). (Pap stain, Mag ×200 in a and ×400 in b). C: Macrophages show numerous safety pin-shaped structures with polar thickening of chromatin …
Klebsiella pneumoniae- Pathogenicity and Clinical Manifestations Klebsiella pneumoniae is a gram-negative, encapsulated, non-motile bacterium that is found in the environment and has been associated with pneumonia in the alcoholic and diabetic patient population. K. pneumoniae is also a well-known cause of community-acquired pneumonia. The bacterium typically colonizes human mucosal surfaces of the oropharynx and gastrointestinal (GI) tract. K. pneumoniae is …
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Laboratory Diagnosis of Klebsiella pneumoniae In the setting of pneumonia, infection with K. pneumoniae is confirmed by either sputum culture analysis, blood culture analysis or midstream urine depending upon the illness. The presence of gram-negative rods in Gram-stained smears suggests Klebsiella; they maybe capsulated and non-sporing. Material is inoculated into blood agar and Mac Conkey agar medium and incubated aerobically. Blood …
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– Klebsiella pneumoniae on MacConkey Agar. – Photo By: Angel Beto Gonzalez from Loja, Ecuador. – Lactose positive mucous colonies. – Colonies are about 4 mm in diameter.
Biochemical Test of Klebsiella pneumoniae Characteristics Klebsiella pneumoniae Capsule +ve Catalase +ve Citrate +ve Flagella -ve Gas +ve Gelatin Hydrolysis -ve Gram Staining -ve Growth in KCN +ve H2S -ve Indole -ve Motility -ve MR (Methyl Red) -ve MUG Test +ve Nitrate Reduction +ve OF (Oxidative-Fermentative) Fermentative Oxidase -ve Pigment -ve Shape Rod Spore -ve TSIA (Triple Sugar Iron Agar) …
Habitat and Morphology of Klebsiella pneumoniae Habitat of Klebsiella pneumoniae They are found in mouth, skin and intestinal tract of human beings. They are also found in soil, water and sewage. Also found in bowel and respiratory tract of humans and animals. Found in the hands of hospital personnel. They are responsible for community and hospital acquired infections. People requiring equipment’s …