Online Microbiology and Biology Study Notes
Biochemical Test of Bacteria Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are necessary for the bacterium’s individual …
Klebsiella pneumoniae- Pathogenicity and Clinical Manifestations Klebsiella pneumoniae is a gram-negative, encapsulated, non-motile bacterium that is found in the environment and has been associated with pneumonia in the alcoholic and diabetic patient population. K. pneumoniae is also a well-known cause of community-acquired pneumonia. The bacterium typically colonizes human mucosal surfaces of the oropharynx and gastrointestinal (GI) tract. K. pneumoniae is …
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Laboratory Diagnosis of Klebsiella pneumoniae In the setting of pneumonia, infection with K. pneumoniae is confirmed by either sputum culture analysis, blood culture analysis or midstream urine depending upon the illness. The presence of gram-negative rods in Gram-stained smears suggests Klebsiella; they maybe capsulated and non-sporing. Material is inoculated into blood agar and Mac Conkey agar medium and incubated aerobically. Blood …
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– Klebsiella pneumoniae on MacConkey Agar. – Photo By: Angel Beto Gonzalez from Loja, Ecuador. – Lactose positive mucous colonies. – Colonies are about 4 mm in diameter.
Biochemical Test of Klebsiella pneumoniae Characteristics Klebsiella pneumoniae Capsule +ve Catalase +ve Citrate +ve Flagella -ve Gas +ve Gelatin Hydrolysis -ve Gram Staining -ve Growth in KCN +ve H2S -ve Indole -ve Motility -ve MR (Methyl Red) -ve MUG Test +ve Nitrate Reduction +ve OF (Oxidative-Fermentative) Fermentative Oxidase -ve Pigment -ve Shape Rod Spore -ve TSIA (Triple Sugar Iron Agar) …
Habitat and Morphology of Klebsiella pneumoniae Habitat of Klebsiella pneumoniae They are found in mouth, skin and intestinal tract of human beings. They are also found in soil, water and sewage. Also found in bowel and respiratory tract of humans and animals. Found in the hands of hospital personnel. They are responsible for community and hospital acquired infections. People requiring equipment’s …