Spot Indole Test- Objective, Principle, Procedure, Results and Limitations

Result Interpretation of Spot Indole Test

Objective of Spot Indole Test To determine the ability of organism for the production of indole by the action of tryptophanase enzyme. Principle of Spot Indole Test Intracellular enzyme tryptophanase mediate the production of indole by hydrolytic activity against amino acid Tryptophan. Bacteria that produce the enzyme tryptophanase are able to degrade the amino acid tryptophan into … Read moreSpot Indole Test- Objective, Principle, Procedure, Results and Limitations

Indole Test- Objective, Principle, Media, Procedure and Results

Result Interpretation of Indole Test

Objective of Indole Test To determine the ability of organism for the production of indole by the action of tryptophanase enzyme.  Principle of Indole Test Tryptophan, an essential amino acid, is oxidized by some bacteria by the action of trytophanase enzyme. Bacteria that produce the enzyme tryptophanase are able to degrade the amino acid tryptophan into … Read moreIndole Test- Objective, Principle, Media, Procedure and Results