Indirect ELISA- Introduction, Steps, Advantages and Protocol

Indirect ELISA- Introduction, Steps, Advantages and Protocol

Introduction Indirect ELISA is a two-step ELISA which involves two binding process of primary antibody and labeled secondary antibody. The primary antibody is incubated with the antigen followed by the incubation with the secondary antibody. However, this may lead to nonspecific signals because of cross-reaction that the secondary antibody may cause. In the indirect ELISA test, the … Read more Indirect ELISA- Introduction, Steps, Advantages and Protocol

Enzyme-linked immunosorbent assay (ELISA)

Enzyme-linked immunosorbent assay (ELISA) and its types

Enzyme-linked immunosorbent assay (ELISA) Enzyme-linked immunosorbent assay (ELISA) utilizes an enzyme system to show specific combination of an antigen with its antibody. It is a method of quantifying an antigen immobilized on a solid surface. ELISA uses a specific antibody with a covalently coupled enzyme. The amount of antibody that binds the antigen is proportional … Read more Enzyme-linked immunosorbent assay (ELISA)