Counter Current Immunoelectrophoresis

Counter Current Immunoelectrophoresis

Counter Current Immunoelectrophoresis Immunoelectrophoresis refers to the process in which an antigen mixture is first separated into its component parts by electrophoresis and then tested by immunodiffusion. The electrophoresed antigen mixture in agarose gel allows the separation of different antigens along the gel slide, and then the lateral diffusion of an antibody in the gel … Read moreCounter Current Immunoelectrophoresis

Rocket Immunoelectrophoresis- Objectives, Principle, Procedure, Results, Applications, Advantages and Limitations

Result Interpretation of Rocket Immunoelectrophoresis

Rocket Immunoelectrophoresis is an adaptation of radial immunodiffusion developed by Laurell. It is also known as electroimmunoassay or electroimmunodiffusion. It is called as “rocket electrophoresis” due to the appearance of the precipitin bands in the shape of cone-like structures (rocket appearance) at the end of the reaction. In rocket immunoelectrophoresis, antigen migrates in an electric field … Read moreRocket Immunoelectrophoresis- Objectives, Principle, Procedure, Results, Applications, Advantages and Limitations

Immunoelectrophoresis- Principle, Procedure, Results and Applications, Advantages and Limitations

Immunoelectrophoresis- Principle, Procedure, Results and Applications

Immunoelectrophoresis refers to precipitation in agar under an electric field. It is a process of combination of immuno-diffusion and electrophoresis. An antigen mixture is first separated into its component parts by electrophoresis and then tested by double immuno-diffusion. Antigens are placed into wells cut in a gel (without antibody) and electrophoresed. A trough is then … Read moreImmunoelectrophoresis- Principle, Procedure, Results and Applications, Advantages and Limitations