Gel Filtration Chromatography

Gel Filtration Chromatography

Gel Filtration Chromatography Biomolecules are purified using different techniques that separate them according to the differences in their specific properties such as size, hydrophobicity, biorecognition, charge, etc. Gel filtration is a technique in which the separation of components is based on the difference in molecular weight or size. It is the simplest and mildest of … Read moreGel Filtration Chromatography

Gel Permeation Chromatography

Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography is also called as gel filtration or size exclusion chromatography. In size exclusion chromatography, the stationary phase is a porous matrix made up of compounds like cross-linked polystyrene, cross-like dextrans, polyacrylamide gels, agarose gels, etc. The separation is based on the analyte molecular sizes since the gel behaves like … Read moreGel Permeation Chromatography