Biochemical Test of Bacteria

Biochemical Test of Bacteria

Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are necessary for the bacterium’s individual metabolism, and some facilitate …

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Biochemical Test of Gardnerella vaginalis

Biochemical Test of Gardnerella vaginalis

Image Source: House Call, MD Some of the characteristics are as follows: Basic Characteristics Properties (Gardnerella vaginalis) Capsule Negative (-ve) Catalase Negative (-ve) Flagella Negative (-ve) Gas Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram negative (-ve) or Gram variable H2S Negative (-ve) Hemolysis Positive (+ve) (Human or Rabbit Blood) Indole Negative (-ve) Motility Negative (-ve) MR (Methyl Red) …

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Habitat and Morphology of Gardnerella vaginalis

Habitat and Morphology of Gardnerella vaginalis

Habitat of Gardnerella vaginalis Normal flora in 40% female (small amount) Found in urine Rarely in blood and wound Found in the anorectal flora of healthy adults of both sexes and as well as children. Isolated from normal vagina of reproductive age female & male urethra (partners of women with bacterial vaginosis). Optimum pH for growth in between 6 and 7. …

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Laboratory diagnosis of Bacterial vaginosis caused by Gardnerella vaginalis

Laboratory diagnosis of Bacterial vaginosis caused by Gardnerella vaginalis Specimen: Vaginal discharge specimen A. Direct Microscopic Examination Bacterial Vaginosis (BV) is characterized by a foul-smelling discharge. A diagnosis/confirmation is done microscopically by examination of gram stains to detect change in vaginal ecology. A smear of vaginal fluid or vaginal swab is gram stained which reveals the presence of Gram negative …

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