Online Microbiology and Biology Study Notes
Objectives of Pyruvate Broth Test To determine the ability of an organism to utilize pyruvate. It aids in the differentiation between Enterococcus faecalis and Enterococcus faecium. Principle of Pyruvate Broth Test In the identification of microorganisms, the widely used method is the ability of bacteria to form organic compounds by metabolizing certain carbohydrates and related compounds. Pyruvate broth is a carbohydrate-free, nutrient-limited …
Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are necessary for the bacterium’s individual metabolism, and some facilitate …
Some of the characteristics are as follows: Basic Characteristics Properties (Enterococcus faecium) 40% Bile Salts Positive (+ve) Capsule Negative (-ve) Catalase Negative (-ve) Citrate Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Gram-positive H2S Negative (-ve) Hemolysis Variable 0.1% Methylene blue milk Positive (+ve) Motility Negative (-ve) 6.5% NaCl Positive (+ve) Pigment Negative (-ve) Shape Cocci Spore Negative (-ve) VP …