Most of the immunological techniques are based on antigen-antibody reactions. Precipitation reactions are one of the important reactions that occur when antigen and antibody come to contact. When a soluble … Read more
Enzyme-Linked Immunosorbent Assay (ELISA) is a modern molecular technique for the detection of antigen-antibody interaction with the help of an enzyme. It is one of the sensitive enzyme immunoassay techniques for the detection … Read more
Introduction Indirect ELISA is a two-step ELISA which involves two binding process of primary antibody and labeled secondary antibody. The primary antibody is incubated with the antigen followed by the incubation with … Read more
The sandwich ELISA quantify antigens between two layers of antibodies (i.e. capture and detection antibody). Sandwich ELISA is named so as antigen is sandwiched between two antibodies. The sandwich assay … Read more
Competitive ELISA is a technique used for the estimation of antibodies present in a specimen, such as serum. Principle of the test is that two specific antibodies, one conjugated with … Read more
