Competitive ELISA Protocol and Animation

Competitive ELISA Protocol and Animation

Competitive ELISA is a technique used for the estimation of antibodies present in a specimen, such as serum. Principle of the test is that two specific antibodies, one conjugated with enzyme and the other present in test serum (if serum is positive for antibodies), are used. Competition occurs between the two antibodies for the same … Read moreCompetitive ELISA Protocol and Animation

Sandwich ELISA- Steps and Advantages

Sandwich ELISA- Steps and Advantages

The sandwich ELISA quantify antigens between two layers of antibodies (i.e. capture and detection antibody). Sandwich ELISA is named so as antigen is sandwiched between two antibodies. The sandwich assay uses two different antibodies that are reactive with different epitopes on the antigen with a concentration that needs to be determined. The antigen to be … Read moreSandwich ELISA- Steps and Advantages

Indirect ELISA- Introduction, Steps, Advantages and Protocol

Indirect ELISA- Introduction, Steps, Advantages and Protocol

Indirect ELISA is a two-step ELISA which involves two binding process of primary antibody and labeled secondary antibody. The primary antibody is incubated with the antigen followed by the incubation with the secondary antibody. However, this may lead to nonspecific signals because of cross-reaction that the secondary antibody may cause. In the indirect ELISA test, the sample … Read moreIndirect ELISA- Introduction, Steps, Advantages and Protocol

Enzyme-linked immunosorbent assay (ELISA)

Enzyme-linked immunosorbent assay (ELISA) and its types

Enzyme-linked immunosorbent assay (ELISA) utilizes an enzyme system to show specific combination of an antigen with its antibody. It is a method of quantifying an antigen immobilized on a solid surface. ELISA uses a specific antibody with a covalently coupled enzyme. The amount of antibody that binds the antigen is proportional to the amount of … Read moreEnzyme-linked immunosorbent assay (ELISA)