Polyacrylamide Gel Electrophoresis (PAGE)

Polyacrylamide Gel Electrophoresis (PAGE)

Electrophoresis through agarose or polyacrylamide gels is a standard method used to separate, identify and purify biopolymers, since both these gels are porous in nature. Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking … Read more

Pulsed Field Gel Electrophoresis (PFGE)

The Method of Pulsed Field Gel Electrophoresis (PFGE)

Pulsed Field Gel Electrophoresis (PFGE) is a technique used for the separation of large deoxyribonucleic acid (DNA) molecules by applying to a gel matrix an electric field that periodically changes direction. As DNA larger than 15-20kb migrating through a gel … Read more

What is Western blotting?

Western blotting- Introduction, Principle and Applications

Introduction Western blot is the analytical technique used in molecular biology, immunogenetics, and other molecular biology to detect specific proteins in a sample of tissue homogenate or extract. Western blotting is called so as the procedure is similar to Southern … Read more