Introduction Western blot is the analytical technique used in molecular biology, immunogenetics, and other molecular biology to detect specific proteins in a sample of tissue homogenate or extract. Western blotting is called so as the procedure is similar to Southern … Read more
Electrophoresis through agarose or polyacrylamide gels is a standard method used to separate, identify and purify biopolymers, since both these gels are porous in nature. Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking … Read more
Pulsed Field Gel Electrophoresis (PFGE) is a technique used for the separation of large deoxyribonucleic acid (DNA) molecules by applying to a gel matrix an electric field that periodically changes direction. As DNA larger than 15-20kb migrating through a gel … Read more
Rocket Immunoelectrophoresis is an adaptation of radial immunodiffusion developed by Laurell. It is also known as electroimmunoassay or electroimmunodiffusion. It is called as “rocket electrophoresis” due to the appearance of the precipitin bands in the shape of cone-like structures (rocket … Read more
Immunoelectrophoresis refers to precipitation in agar under an electric field. It is a process of a combination of immuno-diffusion and electrophoresis. An antigen mixture is first separated into its component parts by electrophoresis and then tested by double immuno-diffusion. Antigens … Read more