Polyacrylamide Gel Electrophoresis (PAGE)

Polyacrylamide Gel Electrophoresis (PAGE)

Polyacrylamide Gel Electrophoresis (PAGE) Electrophoresis through agarose or polyacrylamide gels is a standard method used to separate, identify and purify biopolymers, since both these gels are porous in nature. Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking agent, usually N,N’-methylenebisacrylamide. The reaction is a free radical polymerization, usually … Read morePolyacrylamide Gel Electrophoresis (PAGE)

Pulsed Field Gel Electrophoresis (PFGE)

The Method of Pulsed Field Gel Electrophoresis (PFGE)

Pulsed Field Gel Electrophoresis (PFGE) Pulsed Field Gel Electrophoresis (PFGE) is a technique used for the separation of large deoxyribonucleic acid (DNA) molecules by applying to a gel matrix an electric field that periodically changes direction. As DNA larger than 15-20kb migrating through a gel essentially moves together in a size-independent manner, the standard gel … Read morePulsed Field Gel Electrophoresis (PFGE)

Rocket Immunoelectrophoresis- Objectives, Principle, Procedure, Results, Applications, Advantages and Limitations

Result Interpretation of Rocket Immunoelectrophoresis

Rocket Immunoelectrophoresis is an adaptation of radial immunodiffusion developed by Laurell. It is also known as electroimmunoassay or electroimmunodiffusion. It is called as “rocket electrophoresis” due to the appearance of the precipitin bands in the shape of cone-like structures (rocket appearance) at the end of the reaction. In rocket immunoelectrophoresis, antigen migrates in an electric field … Read moreRocket Immunoelectrophoresis- Objectives, Principle, Procedure, Results, Applications, Advantages and Limitations

Immunoelectrophoresis- Principle, Procedure, Results and Applications, Advantages and Limitations

Immunoelectrophoresis- Principle, Procedure, Results and Applications

Immunoelectrophoresis refers to precipitation in agar under an electric field. It is a process of combination of immuno-diffusion and electrophoresis. An antigen mixture is first separated into its component parts by electrophoresis and then tested by double immuno-diffusion. Antigens are placed into wells cut in a gel (without antibody) and electrophoresed. A trough is then … Read moreImmunoelectrophoresis- Principle, Procedure, Results and Applications, Advantages and Limitations

Western blotting- Introduction, Principle and Applications

Western blotting- Introduction, Principle and Applications

Western blot is the analytical technique used in molecular biology, immunogenetics and other molecular biology to detect specific proteins in a sample of tissue homogenate or extract. Western blotting is called so as the procedure is similar to Southern blotting. While Southern blotting is done to detect DNA, Western blotting is done for the detection … Read moreWestern blotting- Introduction, Principle and Applications