Batch vs Fed-Batch vs Continuous Culture- 20 Key Differences

Batch vs Fed-Batch vs Continuous Culture

Batch Culture Definition In batch culture, all the medium components are placed in the reactor at the start of cultivation except for atmospheric gases, acid or base for pH control, and antifoaming agents. There is a continuous change in the nutrient concentrations over time, and the system remains unsteady. Fed-Batch Culture Definition In simple words, fed-batch culture is a modification to batch fermentation. In fed-batch cultivation, nutrients are added aseptically; it is a semi-open system, and the volume of liquid … Read more

Continuous Culture- Definition, Principle, Process, Types, Applications, Limitations

Continuous Culture

Continuous Culture Definition Continuous culture is a continuous process where nutrients are continually added to the bioreactor and the culture broth (containing cells and metabolites) is removed at the same time. The volume of the culture broth is constant due to a constant feed-in and feed-out rate (i.e consumed nutrients are replaced and toxic metabolites are removed from the culture). Some advantages of continuous culture are: In continuous culture, keeping the working volume constant simplifies culture scale-up based on a … Read more

Fed-batch Culture- Definition, Principle, Process, Types, Applications, Limitations

Fed-batch Culture

Fed-batch culture Definition In simple words, fed-batch culture is a modification to batch fermentation. In fed-batch cultivation, nutrients are added aseptically; it is a semi-open system, and the volume of liquid culture in the bioreactor increases as the culture is systematically added. A fed-batch culture is more productive, yields better with controlled sequential additions of nutrients, enables higher cell densities, and prolongs product synthesis. The main advantages of fed-batch over batch culture are: Long-term synthesis of products,  By increasing the … Read more

Batch Culture- Definition, Principle, Process, Applications, Limitations

Batch Culture

Batch Culture Definition In batch fermentation, all the medium components are placed in the reactor at the start of cultivation except for atmospheric gases, acid or base for pH control, and antifoaming agents. There is a continuous change in the nutrient concentrations over time, and the system remains unsteady. The microbial metabolites may be produced at a primary or secondary stage of the microbial cultivation period. Fermentation is terminated when either all the nutrient is exhausted or the desired concentration of product is achieved. Batch culture has … Read more