Cultural Characteristics

Cultural and Biochemical characteristics of Staphylococcus aureus

Cultural characteristics of Staphylococcus aureus Staphylococci grow readily on most bacteriologic media under aerobic or microaerophilic conditions. Colonies on solid media are round, smooth, raised, and glistening. S. aureus usually forms gray to deep golden yellow colonies. Mannitol Salt Agar: circular, 2–3 mm in diameter, with a smooth, shiny surface; colonies appear opaque and are often … Read moreCultural and Biochemical characteristics of Staphylococcus aureus

Cultural characteristics of Clostridium perfringens

Cultural characteristics of Clostridium perfringens

Cultural characteristics of Clostridium perfringens It is an anaerobic bacteria but can grow under micro-aerophilic condition. They form large, translucent, flat and filamentous colonies with irregular edges. PH: 5.5 to 8.0 (Average PH: 7.2) Temperature: 20°C to 50°C (Average: 37°C) At 45°C, generation time is 10 minutes. Clostridium perfringens on Robertson’s Cooked Meat Broth Saccharolytic … Read moreCultural characteristics of Clostridium perfringens

Cultural Characteristics of E. coli

Cultural Characteristics of E. coli

Cultural Characteristics of E. coli 1. E. coli is facultative anaerobes. 2. Its optimum growth temperature is 37°C and ranges from 10°C to 40°C. E. coli on Nutrient Agar (NA) 1. They appear large, circular, low convex, grayish, white, moist, smooth and opaque. 2. They are of 2 forms: Smooth (S) form and Rough (R) … Read moreCultural Characteristics of E. coli

Cultural Characteristics of Bacillus cereus

Cultural Characteristics of Bacillus cereus

Cultural Characteristics of Bacillus cereus Most Bacillus spp grow readily on nutrient agar or peptone media. The optimum temperature for growth varies from 20°C to 40°C, mostly 37°C. B. cereus is mesophilic and is capable of adapting to a wide range of environmental conditions. On Nutrient Agar at 37°C, it forms large (2-5 mm) grey-white, … Read moreCultural Characteristics of Bacillus cereus

Cultural Characteristics of Mycobacterium tuberculosis

Cultural Characteristics of Mycobacterium tuberculosis

Cultural Characteristics of Mycobacterium tuberculosis Culture is the gold standard for laboratory confirmation of Tuberculosis. Growing bacteria are required to perform drug-susceptibility testing and genotyping. In Lowenstein-Jensen (LJ) medium It is an egg based medium and growth is quite slow. It takes 6-8 weeks to get visual colonies on this type of media. Colonies are … Read moreCultural Characteristics of Mycobacterium tuberculosis