Cultural Characteristics Archives

Enterobacteriaceae Cultural Characteristics

June 18, 2020 by Anupama Sapkota

Image Source: Cole-Parmer Instrument Company. Cultural Characteristics of Citrobacter freundii Citrobacter freundii on Nutrient Agar Smooth, convex, translucent, or opaque grey colored with a shiny surface and entire margin; mucoid or rough colonies occasionally. Citrobacter freundii on Blood Agar Circular, flat, entire red colored colonies. Citrobacter freundii on Mac-Conkey Agar Pink colored after 24 hr incubation; pale-colored colonies turn pink …

Cultural Characteristics of E. coli

January 3, 2020July 26, 2019 by Sagar Aryal

Cultural Characteristics of E. coli 1. E. coli is a facultative anaerobes. 2. Its optimum growth temperature is 37°C and ranges from 10°C to 40°C. E. coli on Nutrient Agar (NA) 1. They appear large, circular, low convex, grayish, white, moist, smooth and opaque. 2. They are of 2 forms: Smooth (S) form and Rough (R) form. 3. Smooth forms …

Cultural Characteristics of Bacillus cereus

January 9, 2020June 11, 2019 by Sagar Aryal

Cultural Characteristics of Bacillus cereus Some of the characteristics are: Most Bacillus spp grow readily on nutrient agar or peptone media. The optimum temperature for growth varies from 20°C to 40°C, mostly 37°C. B. cereus is mesophilic and is capable of adapting to a wide range of environmental conditions. On Nutrient Agar at 37°C, it forms large (2-5 mm) grey-white, …

Cultural Characteristics of Mycobacterium tuberculosis

January 3, 2020May 22, 2019 by Sagar Aryal

Culture is the gold standard for laboratory confirmation of Tuberculosis. Growing bacteria are required to perform drug-susceptibility testing and genotyping. In the Lowenstein-Jensen (LJ) medium It is an egg-based medium and growth is quite slow. It takes 6-8 weeks to get visual colonies on this type of media. Colonies are non-pigmented, dry, rough, raised, irregular with a wrinkled surface They …

Cultural and Biochemical characteristics of Staphylococcus aureus

June 28, 2020October 13, 2017 by Sagar Aryal

Cultural characteristics of Staphylococcus aureus Staphylococci grow readily on most bacteriologic media under aerobic or microaerophilic conditions. Colonies on solid media are round, smooth, raised, and glistening. S. aureus usually forms gray to deep golden yellow colonies. Mannitol Salt Agar: circular, 2–3 mm in diameter, with a smooth, shiny surface; colonies appear opaque and are often pigmented, golden yellow. Tryptic Soy …

Cultural characteristics of Clostridium perfringens

January 11, 2020September 15, 2017 by Sagar Aryal

Cultural characteristics of Clostridium perfringens It is an anaerobic bacteria but can grow under micro-aerophilic condition. They form large, translucent, flat and filamentous colonies with irregular edges. PH: 5.5 to 8.0 (Average PH: 7.2) Temperature: 20°C to 50°C (Average: 37°C) At 45°C, generation time is 10 minutes. Clostridium perfringens on Robertson’s Cooked Meat Broth Saccharolytic species turn meat pink. Meat …