Biochemical Test of Bacteria

Biochemical Test of Bacteria

Biochemical Test of Bacteria Biochemical reactions are very important in the identification of bacterial isolates and in the identification of different bacterial species. These tests depend on the presence of certain enzymes, such as catalase, oxidase, urease, gelatinase, etc., produced by the bacteria. Different bacteria produce varying spectra of enzymes. For example, some enzymes are … Read more Biochemical Test of Bacteria

Loeffler Medium

Loeffler Medium

Loeffler Medium Loeffler Medium is a modification of the original formula developed by Loeffler in 1887. Loeffler medium enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms, especially from nose and throat. It also restores virulence and other identifying properties (microscopic and colonial) after they have been lost due to prolonged incubation or … Read more Loeffler Medium

Biochemical Test of Corynebacterium diphtheriae

Biochemical Test of Corynebacterium diphtheriae

Biochemical Test of Corynebacterium diphtheriae Basic Characteristics Properties (Corynebacterium diphtheriae) CAMP Negative (-ve) Capsule Non-Capsulated Catalase Positive (+ve) Cetrimide Test Negative (-ve) Citrate Negative (-ve) Coagulase Negative (-ve) Flagella Non-Flagellated Gas Variable Gelatin Hydrolysis Negative (-ve) Gram Staining Positive (+ve) H2S Positive (+ve) Hemolysis Weak Beta hemolysis Indole Negative (-ve) Motility Non-Motile MR (Methyl Red) … Read more Biochemical Test of Corynebacterium diphtheriae

Pathogenesis and Clinical manifestations of Corynebacterium diphtheriae

Pathogenesis and Clinical manifestations of Corynebacterium diphtheriae

Pathogenesis of Corynebacterium diphtheriae In nature, C diphtheriae occurs in the respiratory tract, in wounds, or on the skin of infected persons or normal carriers. It is spread by droplets or by contact to susceptible individuals; the bacilli then grow on mucous membranes or in skin abrasions, and those that are toxigenic start producing toxin. … Read more Pathogenesis and Clinical manifestations of Corynebacterium diphtheriae

Laboratory diagnosis, treatment and prevention of Corynebacterium diphtheriae

Laboratory diagnosis, treatment and prevention of Corynebacterium diphtheriae

Laboratory diagnosis of Corynebacterium diphtheriae Specimen Dacron swabs from the nose, throat, or other suspected lesions must be obtained before antimicrobial drugs are administered. Swabs should be collected from beneath any visible membrane. The swab should then be placed in semisolid transport media such as Amies. Microscopy Smears stained with alkaline methylene blue or Gram stain … Read more Laboratory diagnosis, treatment and prevention of Corynebacterium diphtheriae

Habitat and Morphology of Corynebacterium diphtheriae

Habitat and Morphology of Corynebacterium diphtheriae

Habitat and Morphology of Corynebacterium diphtheriae Habitat of Corynebacterium diphtheriae Found in nasopharynx as well as in skin lesions, which actually represent a reservoir for the spread of diphtheria. Also found in mouth, throat, nose, skin and wound of infected person. Animals do not easily contract diphtheria. Patients carry toxigenic organism upto 3 months after infection. … Read more Habitat and Morphology of Corynebacterium diphtheriae